Inhibitive Effects of Alkyl Gallates on Hyaluronidase and Collagenase

Barla, Florin; Higashijima, Hayato; Funai, Shingo; Sugimoto, Keiichiro; Harada, Naoki; Yamaji, Ryoichi; Fujita, Tomoyuki; Nakano, Yoshihisa; Inui, Hiroshi

doi:10.1271/bbb.90365

Cited by 34 publications

(18 citation statements)

References 19 publications

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“…In limited clinical studies, HAase has been used to enhance the efficacy of vinblastin in the treatment of malignant melanoma and Kaposi’s sarcoma, among other cancers [121]. Furthermore, when the level of HA decreases under conditions in which hyaluronidase activity increases, the moisture and tension of the skin are reduced, and histamine is released from mast cells [122]. Therefore, the identification and characterization of hyaluronidase inhibitors could be relevant to the development of contraceptives, as well as anti-tumor, anti-microbial, and anti-venom, anti-wrinkle, and anti-aging agents, and allergy and inflammation suppressors [14,122,123,124].…”

Section: Hyaluronidasementioning

confidence: 99%

“…Furthermore, when the level of HA decreases under conditions in which hyaluronidase activity increases, the moisture and tension of the skin are reduced, and histamine is released from mast cells [122]. Therefore, the identification and characterization of hyaluronidase inhibitors could be relevant to the development of contraceptives, as well as anti-tumor, anti-microbial, and anti-venom, anti-wrinkle, and anti-aging agents, and allergy and inflammation suppressors [14,122,123,124]. Therefore, Loxosceles recombinant hyaluronidases are associated with numerous potential applications [27,74,125,126].…”

Section: Hyaluronidasementioning

confidence: 99%

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Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Chaim

Trevisan-Silva

Chaves-Moreira

et al. 2011

Toxins

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Venomous animals use their venoms as tools for defense or predation. These venoms are complex mixtures, mainly enriched of proteic toxins or peptides with several, and different, biological activities. In general, spider venom is rich in biologically active molecules that are useful in experimental protocols for pharmacology, biochemistry, cell biology and immunology, as well as putative tools for biotechnology and industries. Spider venoms have recently garnered much attention from several research groups worldwide. Brown spider (Loxosceles genus) venom is enriched in low molecular mass proteins (5–40 kDa). Although their venom is produced in minute volumes (a few microliters), and contain only tens of micrograms of protein, the use of techniques based on molecular biology and proteomic analysis has afforded rational projects in the area and permitted the discovery and identification of a great number of novel toxins. The brown spider phospholipase-D family is undoubtedly the most investigated and characterized, although other important toxins, such as low molecular mass insecticidal peptides, metalloproteases and hyaluronidases have also been identified and featured in literature. The molecular pathways of the action of these toxins have been reported and brought new insights in the field of biotechnology. Herein, we shall see how recent reports describing discoveries in the area of brown spider venom have expanded biotechnological uses of molecules identified in these venoms, with special emphasis on the construction of a cDNA library for venom glands, transcriptome analysis, proteomic projects, recombinant expression of different proteic toxins, and finally structural descriptions based on crystallography of toxins.

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Section: Hyaluronidasementioning

confidence: 99%

Section: Hyaluronidasementioning

confidence: 99%

Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Chaim

Trevisan-Silva

Chaves-Moreira

et al. 2011

Toxins

View full text Add to dashboard Cite

show abstract

“…When the level of hyaluronic acid decreased under conditions in which the hyaluronidase activity is increased, the moisture and tension of the skin are reduced. Thus, hyaluronidase inhibitors are useful cosmeceutical ingredients as they have anti-wrinkle and anti-aging effects on the skin [7]. Tyrosinase is known to be a key enzyme for the melanin biosynthesis, the compound largely responsible for hair and skin color in mammals.…”

Section: Introductionmentioning

confidence: 99%

Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes

Chompoo

Upadhyay

Fukuta

et al. 2012

BMC Complement Altern Med

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BackgroundThe skin is chronically exposed to endogenous and environmental pro-oxidant agents, leading to the harmful generation of reactive oxygen species. Antioxidant is vital substances which possess the ability to protect the body from damage cause by free radicals induce oxidative stress. Alpinia zerumbet, a traditionally important economic plant in Okinawa, contains several interesting bioactive constituents and possesses health promoting properties. In this regard, we carried out to test the inhibitory effect of crude extracts and isolated compounds from A. zerumbet on antioxidant and skin diseases-related enzymes.MethodsThe antioxidant activities were examined by DPPH, ABTS and PMS-NADH radical scavenging. Collagenase, elastase, hyaluronidase and tyrosinase were designed for enzymatic activities to investigate the inhibitory properties of test samples using a continuous spectrophotometric assay. The inhibitory capacity of test samples was presented at half maximal inhibitory concentration (IC50).ResultsThe results showed that aqueous extract of the rhizome was found to have greater inhibitory effects than the others on both of antioxidant and skin diseases-related enzymes. Furthermore, 5,6-dehydrokawain (DK), dihydro-5,6-dehydrokawain (DDK) and 8(17),12-labdadiene-15,16-dial (labdadiene), isolated from rhizome, were tested for antioxidant and enzyme inhibitions. We found that DK showed higher inhibitory activities on DPPH, ABTS and PMS-NADH scavenging (IC50 = 122.14 ± 1.40, 110.08 ± 3.34 and 127.78 ± 4.75 μg/ml, respectively). It also had stronger inhibitory activities against collagenase, elastase, hyaluronidase and tyrosinase (IC50 = 24.93 ± 0.97, 19.41 ± 0.61, 19.48 ± 0.24 and 76.67 ± 0.50 μg/ml, respectively) than DDK and labdadiene.ConclusionOur results indicate that the rhizome aqueous extract proved to be the source of bioactive compounds against enzymes responsible for causing skin diseases. Moreover, DK could be used as a potent inhibitor and be further exploited to be used in anti-skin disease formulations.

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“…Both collagenase and lipoxygenase were inhibited by addition of NA. As it was reported that the IC 50 value of anacardic acid for lipoxygenase was 6.8 µM (Tsujimoto et al, 2007) and that of EDTA for collagenase was 580 µM (Barla et al, 2009), we deduced that inhibitory activity of NA was in part associated with its chelation capacity. The complex formations of NA with metals were examined by using negative ESI-MS.…”

Section: Discussionmentioning

confidence: 79%

Characterization of Nicotianamine Isolated from Soybeans

Kuroda¹,

Ishihara²,

Masuoka³

2013

JFR

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Angiotensin І-converting enzyme (ACE) [EC 3.4.15.1] plays one of important roles to control the arterial blood pressure. Inhibitory activity of the enzyme has been reportedly detected in plant foods.We examined the activity in plant foods and isolated an ACE inhibitor in soybeans. The structure was identified as nicotianamine, N- [N-(3-amino-3carboxypropyl)-3-amino-3-carboxypropyl]-azetidine-2-carboxylic acid. The contents in several soybean foods were estimated using an amino acid analyzer. The chelation capacity of nicotianamine with metals was examined using a mass spectrometer.Soybean foods contained considerable amounts of nicotianamine but fermentation caused a decrease in the nicotianamine content. The (1:1) adduct formation of nicotianamine with metals was directly observed in the mass spectra.

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Inhibitive Effects of Alkyl Gallates on Hyaluronidase and Collagenase

Cited by 34 publications

References 19 publications

Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Brown Spider (Loxosceles genus) Venom Toxins: Tools for Biological Purposes

Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes

Characterization of Nicotianamine Isolated from Soybeans

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