Inhibitor formation and detoxification during lignocellulose biorefinery: A review

Guo, Hongliang; Zhao, Ying; Chang, Jo‐Shu; Lee, Dong Hoon

doi:10.1016/j.biortech.2022.127666

Cited by 64 publications

(18 citation statements)

References 118 publications

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“…This decrease was attributed to the inhibitors present in lignocellulose hydrolysate . The enzymatic hydrolysis of lignocellulose releases toxic substances, including carboxylic acids and furfural, which can inhibit sugar uptake, microbial growth, and metabolism . As shown in Figure , no xylitol accumulation was detected in the fermentation broth.…”

Section: Resultsmentioning

confidence: 98%

Glycerol Production from Undetoxified Lignocellulose Hydrolysate by a Multiresistant Engineered Candida glycerinogenes

Jiang,

Wang,

Zhao

et al. 2024

J. Agric. Food Chem.

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Glycerol is an important platform compound with multidisciplinary applications, and glycerol production using low-cost sugar cane bagasse hydrolysate is promising. Candida glycerinogenes, an industrial yeast strain known for its high glycerol production capability, has been found to thrive in bagasse hydrolysate obtained through a simple treatment without detoxification. The engineered C. glycerinogenes exhibited significant resistance to furfural, acetic acid, and 3,4-dimethylbenzaldehyde within undetoxified hydrolysates. To further enhance glycerol production, genetic modifications were made to Candida glycerinogenes to enhance the utilization of xylose. Fermentation of undetoxified bagasse hydrolysate by CgS45 resulted in a glycerol titer of 40.3 g/L and a yield of 40.4%. This process required only 1 kg of bagasse to produce 93.5 g of glycerol. This is the first report of glycerol production using lignocellulose, which presents a new way for environmentally friendly industrial production of glycerol.

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Section: Resultsmentioning

confidence: 98%

Glycerol Production from Undetoxified Lignocellulose Hydrolysate by a Multiresistant Engineered Candida glycerinogenes

Jiang,

Wang,

Zhao

et al. 2024

J. Agric. Food Chem.

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“…Inhibitory compounds in biomass hydrolysate : There is a chance that several inhibitory substances such as phenolic acids, pyrroles, and carboxylic acids could occur during the pretreatment of lignocellulosic biomass [ 183 ]. Hydrolysis and fermentation performance can be decreased by these inhibitors.…”

Section: Current Status Challenges and Perspectivesmentioning

confidence: 99%

“…Pre-treatment sometimes requires chemicals that are difficult to recover or recycle. Therefore, this process is energy-intensive and not cost-efficient [ 183 ]. Additionally, some chemicals are harmful to human health and the environment.…”

Section: Current Status Challenges and Perspectivesmentioning

confidence: 99%

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

Khunnonkwao,

Thitiprasert,

Jaiaue

et al. 2024

Heliyon

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“…The general detoxification methods such as water-washing, activated carbon adsorption, and overliming inevitably result in much loss of fermentable sugars or generation of wastewater . For practical biorefinery processes under high lignocellulose solids loading conditions, biological detoxification (biodetoxification) using specific microorganisms is considered as the only proper approach among various available options by its effectiveness on complete degradation of inhibitors into CO 2 and water with the good preservation of fermentable sugars. − …”

Section: Introductionmentioning

confidence: 99%

Changes in pH Values Allow for a Visible Detection of the End Point in Submerged Liquid Biodetoxification during Biorefinery Processing

Han,

Zhang,

Yang

et al. 2023

ACS Sustainable Chem. Eng.

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Detoxification of inhibitory compounds in pretreated lignocellulose feedstock is a crucial step prior to consequent fermentations in the biorefinery chain. Biological detoxification offers a fast and highly selective method for biodegradation of inhibitory compounds into CO 2 and water. This study focused on the enhanced online monitoring of the end point of the submerged liquid biodetoxification by Paecilomyces variotii FN89 in high solids loading wheat straw hydrolysate. Submerged liquid biodetoxification reduces reactor volume and improves mass transfer with greater ease of operation but faces the challenge of timely switching of the biodetoxification stage into the fermentation stage of biodetoxification microbes in the lignocellulose hydrolysate with highly concentrated sugars and inhibitors. In other words, the challenge is to quickly identify the end point of biodetoxification to ensure a timely completion of inhibitors while maintaining the fermentable sugars untouched. The inadequate biodetoxification results in poor fermentability in the subsequent bioconversion step, while excessive biodetoxification leads to the unwanted consumption of fermentable sugars. The results indicate that all furfural and 5-hydroxymethylfurfural (HMF) and over 80% of acetic acid in the wheat straw hydrolysate were effectively removed within a short period (12−24 h) under moderate operating parameters and negligible loss of fermentable sugars (below 2%). We observed that the change of pH value had a certain regularity and exactly corresponded to the biodetoxification process due to the consumption of inhibitory acids (acetic acid) and the generation of acidic byproducts (fatty acids). Upon reaching the maximum pH value through the visible online observation, the biodetoxification stage could be promptly transitioned to the fermentation stage by switching-off the aeration, inoculating the fermentation microbe seed and changing the fermentation parameters. The method was applied to the productions of chiral L-lactic acid, ethanol, and sugar acids from wheat straw after dry acid pretreatment, enzymatic saccharification, simultaneous saccharification, and co-fermentation. This pH peak value can be used as a timely, easily visible, and detectable indicator of submerged liquid biodetoxification completion with rapid and complete removal of inhibitors and negligible loss of fermentable sugars.

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Inhibitor formation and detoxification during lignocellulose biorefinery: A review

Cited by 64 publications

References 118 publications

Glycerol Production from Undetoxified Lignocellulose Hydrolysate by a Multiresistant Engineered Candida glycerinogenes

Glycerol Production from Undetoxified Lignocellulose Hydrolysate by a Multiresistant Engineered Candida glycerinogenes

The outlooks and key challenges in renewable biomass feedstock utilization for value-added platform chemical via bioprocesses

Changes in pH Values Allow for a Visible Detection of the End Point in Submerged Liquid Biodetoxification during Biorefinery Processing

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