Inhibitors of protein phosphorylation including the retinoblastoma protein induce germination of<i>Candida albicans</i>

Cho, T.; Hamatake, H.; Hagihara, Yoshisato; Kaminishi, Hidenori

doi:10.1080/mmy.38.1.41.45

Cited by 11 publications

(1 citation statement)

References 20 publications

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“…From the results, possible sources of fungal airborne contaminants increasing microbial levels may be attributable to the high level of human activity observed during the fourth sampling round that resulted to a need to open windows, and possibly to the introduction of outdoor fungi to the indoor areas. Other possible sources include inadequate air filtration systems: insufficient air filtering may provide easy access to the hospital indoor environment for mould spores [5,21]. Additional studies to assess the efficacy of the air filtration systems shall have to be assessed in future.…”

Section: Resultsmentioning

confidence: 99%

Identification of airborne microbiota in selected areas in a health-care setting in South Africa

et al. 2014

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BackgroundThe role of bio-aerosols in the spread of disease and spoilage of food has been described in numerous studies; nevertheless this information at South African hospitals is limited. Attributable to their size, bio-aerosols may be suspended in the air for long periods placing patients at risk of infection and possibly settling on surfaces resulting in food contamination. The aim of the study is to assess the microbial composition of the air in the kitchen and selected wards at a typical district hospital in South Africa. Air samples were collected using the settle plates and an SAS Super 90 air sampler by impaction on agar. These microbial samples were quantified and identified using Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) and Analytic Profile Index (API).ResultsMicrobial counts were found to be higher in the fourth (≤6.0 × 101 cfu/m-3) sampling rounds when compared to the first (≥2 cfu/m-3), second (≤3.0 × 101 cfu/m-3) and third (≤1.5 × 101 cfu/m-3) sampling rounds. Genera identified included Bacillus, Kocuria, Staphylococcus, Arthrobacter, Candida, Aureobasidium, Penicillium and Phoma amongst others. The presence of these pathogens is of concern, attributable to their ability to cause diseases in humans especially in those with suppressed host immunity defenses. Furthermore, fungal genera identified (e.g. Candida) in this study are also known to cause food spoilage and fungal infections in patients.ConclusionResults from this study indicate the importance of air quality monitoring in health-care settings to prevent possible hospital-acquired infections and contamination of hospital surfaces including food contact surfaces by airborne contaminants.

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Section: Resultsmentioning

confidence: 99%

Identification of airborne microbiota in selected areas in a health-care setting in South Africa

et al. 2014

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Isolation and sequencing of the Candida albicans MSI3, a putative novel member of the HSP70 family

Cho

Toyoda

Sudoh

et al. 2002

Yeast

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We have reported previously that the expression of CGR1 increased at an early stage of the yeast-mycelial transition (morphogenesis) in Candida albicans. We now show that Cgr1p interacts in a yeast two-hybrid system with the C. albicans Msi3p (CaMsi3p), a putative novel member of the heat shock protein 70 (HSP70) family. The DNA sequence of CaMSI3 encodes a predicted protein of 702 amino acids with a molecular mass of 78.6 kDa. The amino acid sequence of CaMsi3p is 63% identical to Msi3p/Sse1p of the HSP70 family of Saccharomyces cerevisiae. Further, CaMSI3 complemented the temperature-sensitive phenotype of the msi3 − mutant of S. cerevisiae. Other heat shock proteins of C. albicans are required for morphogenesis and are highly antigenic. These observations suggest that CaMSI3 may well provide functions for this organism unrelated to a heat shock function. The DDBJ Accession No. for the sequence reported in this paper is AB061274.

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Phosphorylation of glucosamine-6-phosphate synthase is important but not essential for germination and mycelial growth of Candida albicans

Gabriel

Olchowy

Stanisławska-Sachadyn

et al. 2004

FEMS Microbiology Letters

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A site-directed mutagenesis of the GFA1 gene encoding Candida albicans glucosamine-6-phosphate (GlcN-6-P) synthase afforded its GFA1S208A version. A product of the modified gene, lacking the putative phosphorylation site for protein kinase A (PKA), exhibited all the basic properties identical to those of the wild-type enzyme but was no longer a substrate for PKA. Comparison of the C. albicans Deltagfa1/GFA1 and Deltagfa1/GFA1S208A cells, grown under conditions stimulating yeast-to-mycelia transformation, revealed that the latter demonstrated lower GlcN-6-P synthase specific activity, decreased chitin content and formed much fewer mycelial forms. All these findings, as well as the observed effects of specific inhibitors of protein kinases, suggest that a loss of the possibility of GlcN-6-P synthase phosphorylation by PKA strongly reduces but not completely eliminates the germinative response of C. albicans cells.

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Inhibitors of protein phosphorylation including the retinoblastoma protein induce germination ofCandida albicans

Cited by 11 publications

References 20 publications

Identification of airborne microbiota in selected areas in a health-care setting in South Africa

Identification of airborne microbiota in selected areas in a health-care setting in South Africa

Isolation and sequencing of the Candida albicans MSI3, a putative novel member of the HSP70 family

Phosphorylation of glucosamine-6-phosphate synthase is important but not essential for germination and mycelial growth of Candida albicans

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