Inhibitory action of citrinin on cultured hepatoma cells

Lorkowski, G.; Creppy, E. E.; Beck, G. Ε.; Dirheimer, G.; Röschenthaler, R.

doi:10.1016/0015-6264(80)90162-5

Cited by 12 publications

(7 citation statements)

References 10 publications

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“…The CTN action mode at the cellular level is characterized by accumulation in the mitochondria and interference with the electron transport system (Chagas et al, 1992;Riberio et al, 1997). In addition, the syntheses of RNA and DNA were inhibited by CTN in V79-E cells (Wasternack and Weisser, 1992) and in cultured hepatoma cells (Lorkowski et al, 1980).…”

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confidence: 99%

Effects of the mycotoxin citrinin on micronucleus formation in a cytokinesis‐block genotoxicity assay in cultured human lymphocytes

Dönmez-Altuntaş¹,

Dumlupinar²,

İmamoğlu³

et al. 2007

J of Applied Toxicology

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Some mycotoxins produced by microfungi are capable of causing disease and death in animals and humans. In the present study, the mycotoxin citrinin (CTN) was evaluated for its genotoxic effects to human peripheral blood lymphocytes from six different individuals. Lymphocyte cultures were treated for 48 h with CTN at six different concentrations between 10 and 100 microM. Lymphocyte cultures were also incubated with 0.1 microM mitomycin c (MMC) as a positive control, and 0.5% absolute ethanol as a vehicle control.CTN caused a significant concentration-dependent increase in micronucleus (MN) frequency in human lymphocytes. At the 60 microM, 80 microM and 100 microM concentrations, CTN was found to induce MN in cytokinesis-blocked lymphocytes in comparison with negative controls (P = 0.014). All the CTN concentrations also led to a clear decrease in the percentages of binucleated/mononucleated cells (P = 0.014). These results indicate that CTN at high concentrations is genotoxic in cultured human lymphocytes.

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confidence: 99%

Effects of the mycotoxin citrinin on micronucleus formation in a cytokinesis‐block genotoxicity assay in cultured human lymphocytes

Dönmez-Altuntaş¹,

Dumlupinar²,

İmamoğlu³

et al. 2007

J of Applied Toxicology

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“…In vivo inhibition of DNA, RNA, and protein synthesis by citrinin has been reported for S. cerevisiae and B. subtilis (5) and for E. coli and hepatoma culture cells (7,19,20). Betina and Barath (5) found no in vitro inhibition by citrinin of calf thymus DNA polymerase, E. coli DNA polymerase, and of three systems testing the activity of ribosomal peptidyl transferase.…”

Section: Discussionmentioning

confidence: 99%

“…Betina and Barath (5) found an inhibition of nucleic acid synthesis by Saccharomyces cerevisiae and by Bacillus subtilis in vivo. Previously we have shown in vivo inhibition of nucleic acid synthesis in hepatoma culture cells (7,19) and in Escherichia coli (20). However, since Betina and Barath (5) found citrinin to be ineffective in cell-free systems, they suggested that these inhibitions were secondary effects.…”

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confidence: 97%

“…Growth of bacteria is inhibited by citrinin; in fact, citrinin is one of those mycotoxins which were first characterized as antibiotics (26). Most granm-positive bacteria but also some strains of gram-negative bacteria ate sensitive to citrinin, especially at low pH (20). Betina and Barath (5) found an inhibition of nucleic acid synthesis by Saccharomyces cerevisiae and by Bacillus subtilis in vivo.…”

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Action of citrinin on bacterial chromosomal and plasmid DNA in vivo and in vitro

Martin¹,

Lorkowski²,

Creppy³

et al. 1986

Appl Environ Microbiol

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Citrinin, a mycotoxin of Penicillium citrinum and other species of the genera Penicillium and Aspergillus, caused the following effects at different concentrations in Escherichia coli. In vivo at 100 ,ug/mI single-strand breaks were caused in the chromosomal DNA. In the presence of 100 ,ug/ml, UV (254 nm)-induced DNA damage was repaired in the bacterial cells without need for a complete growth medium. At 300 ,ug/ml lambda ts prophage was induced in a lysogenic E. coli strain. In an E. coli strain carrying a F' lac plasmid, 4.7% of the cells displayed the Lac-phenotype after treatment with 200 ,ug of citrinin per ml, suggesting elimination of the F' factor. In vitro, DNA repair synthesis was observed at 5 ,ug of citrinin per ml in permeabilized cells, and replicative DNA synthesis was inhibited at 200 ,ug/ml. In these systems synthesis of stable RNAs was slightly diminished at 300 ,ug/mI, and protein synthesis was not affected at concentrations up to 450 ,ug/ml. Lambda and ColEl plasmid DNA were cleaved in vitro when small amounts of copper ions were present. This DNA-attacking activity was prevented by NADPH, catalase, and superoxide dismutase and by higher concentrations of hydroxyl radical scavengers, suggesting the involvement of free radicals in the mechanism of action of citrinin on DNA.

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“…Citrinina aplicada em cultura de células de hepatoma em doses de até 25 μ ol/L foi avaliada o o itostáti a, já e o e t ações a i a de 0 μ ol/L citotóxica. 58 Quando testaram o efeito de diferentes concentrações da citrinina sobre a viabilidade celular em células Vero, o se va a ue e doses de até 0 μ ol/L não houve mudanças significativas. 59 Os dados sobre carcinogenicidade da citrinina na literatura são escassos.…”

Section: Atividade Biológica E Toxidez Da Citrininaunclassified

History, Applications, Activity and Changes of Citrinin

Cruz

Costa

Figueroa‐Villar

2016

Revista Virtual De Química

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Abstract:Citrinin is a natural product obtained from fungi and with good biological activity. Because this compound also presents toxicity, especially being nephrotoxicity, its structural modification is important to obtain new potential pharmacological agents without toxicity. This article presents the history, obtention methods, synthesis, structural modification, evaluation and biological activity of citrinin.Keywords: Citrinin; Penicillium; mycotoxins; biosynthesis; antibiotic; nephrotoxicity. ResumoA Citrinina é um produto natural obtido de fungos e com boa atividade biológica. Como ela também apresenta certa toxidez, especialmente sendo nefrotóxica, é importante sua modificação estrutural para obter novos fármacos sem essa toxidez. Este artigo apresenta a história, os métodos de obtenção, síntese, modificação estrutural, avaliação e atividade biológica da citrinina.

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Inhibitory action of citrinin on cultured hepatoma cells

Cited by 12 publications

References 10 publications

Effects of the mycotoxin citrinin on micronucleus formation in a cytokinesis‐block genotoxicity assay in cultured human lymphocytes

Effects of the mycotoxin citrinin on micronucleus formation in a cytokinesis‐block genotoxicity assay in cultured human lymphocytes

Action of citrinin on bacterial chromosomal and plasmid DNA in vivo and in vitro

History, Applications, Activity and Changes of Citrinin

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