Inhibitory activities of Ficus benghalensis bark against carbohydrate hydrolyzing enzymes - An in vitro study

Ahmed, Faiyaz; Chavan, Shailesh; Satish, Ann Susan; Punith, Kumar R

doi:10.5530/pj.2011.20.7

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…Isolated 6‐O‐acyl‐β‐D‐glucosyl‐β‐sitosterols (6‐AGS) from F. carica latex showed anticancer activities against many cancer cells such as Burkitt B‐cell lymphoma (DG‐75 cells) and T‐cell leukemia (Jurkat cells) (Rubnov et al., 2001). The fruit of F. carica and bark of F. benghalensis have been reported for their potent antidiabetic properties by inhibiting α‐glucosidase enzyme in vitro (Ahmed et al., 2011; Wojdyło et al., 2016), leading to reduced glucose absorption in the intestinal tract. Ficus spp.…”

Section: Introductionmentioning

confidence: 99%

Health‐promoting bioactivity and in vivo genotoxicity evaluation of a hemiepiphyte fig, Ficus dubia

Suttisansanee

Pitchakarn

Ting

et al. 2021

Food Science & Nutrition

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Ficus species have been used as a typical component in food and folk medicine in Asia for centuries. However, little is known regarding the bioactivity and genotoxicity of the recently identified Ficus dubia (FD), an indigenous plant of the tropical evergreen rain forest. FD is unique from other Ficus species because of its highly sought‐after red‐brown latex. Antioxidant properties together with phenolic and flavonoid contents of FD were elucidated. Health‐promoting characteristics were examined by studying the inhibition of enzymes as a drug target for diabetes, hypertension, Alzheimer's disease, and obesity, together with anticancer ability against human colorectal adenocarcinoma, human hepatocellular carcinoma, human ovarian carcinoma, human prostate adenocarcinoma, and human lung carcinoma. Besides, FD genotoxicity was tested using the Drosophila wing spot test. Results showed that both FD root and latex exhibited antioxidant activity due to the presence of phenolics and flavonoids, specifically caffeic acid and cyanidin. The ethanolic fraction of FD root demonstrated a potent antidiabetic mechanism underlying α‐glucosidase inhibitory activity similar to acarbose. This fraction also suppressed lung and ovarian cancer growth, possibly by G1 and G2/M arrest, respectively. All tested fractions lacked mutagenicity in vivo. Results indicated that FD can be developed as novel antidiabetic compounds; however, its bioactive compounds should be further identified.

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Section: Introductionmentioning

confidence: 99%

Health‐promoting bioactivity and in vivo genotoxicity evaluation of a hemiepiphyte fig, Ficus dubia

Suttisansanee

Pitchakarn

Ting

et al. 2021

Food Science & Nutrition

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“…We therefore believe that the glucose lowering potential demonstrated in vivo by the extract of F. sycomorus may be due to the inhibition of α-glucosidase activity (sucrase). Some authors have shown that the extracts of other Ficus species such as F. racemose [60], F. benghalensis [61] and F. deltoidea [62] inhibit the activity of sucrase with an EC50 of 367 ± 15.2 μg/ml and 239 ± 14.3 μg/ml respectively for cold and hot water extract of F. racemosa , and 193 ± 21.6 μg/ml and 141 ± 22.1 μg/ml respectively for cold and hot water extract of F. benghalensis . This was in agreement with our results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the inhibition of carbohydrate hydrolysing enzymes, antioxidant activity and polyphenolic content of extracts of ten African Ficus species (Moraceae) used traditionally to treat diabetes

Olaokun

McGaw

Eloff

et al. 2013

BMC Complement Altern Med

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BackgroundSome Ficus species have been used in traditional African medicine in the treatment of diabetes. The antidiabetic potential of certain species has been confirmed in vivo but the mechanism of activity remains uncertain. The aim of this study was to determine the activity and to investigate the mechanism of antidiabetic activity of ten selected Ficus species through inhibition of α-amylase and α-glucosidase activity, and the possible relationship between these activities, the total polyphenolic content and the antioxidant activity.MethodsDried acetone leaf extracts were reconstituted with appropriate solvents and used to determine total polyphenolic content antioxidant activity, α-amylase and α-glucosidase inhibitory activity.ResultsThe crude acetone extract of F. lutea had the highest polyphenolic content (56.85 ± 1.82 mg GAE/g of dry material) and the strongest antioxidant activity with a TEAC value of 4.80 ± 0.90. The antioxidant activity of the acetone extracts of the Ficus species may not be ascribed to total polyphenolic content alone. The crude extract at a concentration of 0.5 mg/ml of F. lutea (64.3 ± 3.6%) had the best α-glucosidase (sucrase) inhibitory activity. The EC50 of F. lutea (290 ± 111 μg/ml) was not significantly different from that of F. sycomorus (217 ± 69 μg/ml). The α-amylase inhibitory activity of F. lutea (95.4 ± 1.2%) at a concentration of 1 mg/ml was the highest among the Ficus species screened. The EC50 for F. lutea (9.42 ± 2.01 μ g/ml), though the highest, was not significantly different (p < 0.05) from that of F. craterostoma and F. natalensis. It was apparent that the crude acetone extract of F. lutea is a partially non-competitive inhibitor of α-amylase and α-glucosidase. Based on correlation coefficients polyphenolics may be responsible for α-glucosidase activity but probably not for α-amylase activity.ConclusionAntidiabetic activity potential via inhibition of α-amylase and α-glucosidase was discovered in Ficus lutea which has not been previously reported. The acetone extract of the leaves was high in total polyphenolic content and antioxidant activity, and was a potent inhibitor of α-amylase activity. Research is underway to isolate the active compound(s) responsible for the antidiabetic activity and to confirm the in vitro antidiabetic activity and to investigate in vitro toxicity.

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“…The liberation of glucose was greatly inhibited by FRB in amylolysis kinetics. Furthermore, it increased the rate of glucose transport across the yeast cell membrane and in isolated rat hemi-diaphragm and inhibited α-amylase, α-glucosidase, β-glucosidase and sucrase in a dose dependent manner indicating a strong hypoglycemic effect in vitro and ex vivo [13][14][15]. Similar observations were noted with reference to Morus indica, wherein the sample effectively inhibited the digestion of starch and also inhibited the movement of liberated glucose across the dialysis membrane which could be attributed to the inhibition of carbohydrate hydrolyzing enzymes.…”

Section: Pre-clinical Studiesmentioning

confidence: 99%

Capillary Liquid Chromatographic Fingerprint for Identification and Authentication of from Its Related Species

Rafi¹,

Lim²,

Takeuchi³

2013

TOPROCJ

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Despite the great strides made in the understanding and management of diabetes, the disease and diseaserelated complications are increasing unabated. Phytochemicals identified from traditional medicinal plants are presenting an exciting opportunity for the development of new types of therapeutics. Our research group has explored the possible mechanisms of antidiabetic action of two medicinal plants viz., Ficus racemosa bark (FRB) and Morus indica leaves (MI), known to possess antihyperglycemic effect using simple in vitro techniques followed by pre-clinical and clinical studies. Both plants exhibited good glucose adsorption capacity, retarded glucose diffusion, inhibited enteric enzymes viz., α-amylase, α-glucosidase and sucrase emphasizing that inhibition of carbohydrate hydrolyzing enzymes is one of the mechanisms of hypoglycemic action of FRB and MI. Further, both FRB and MI demonstrated significant antihyperglycemic activity in streptozotocin-induced diabetic rats associated with significant elevation in serum insulin. The samples also exhibited cholesterol and triglyceride lowering effect. In clinical trials, the antihyperglycemic effect was consistent which resulted in good glycemic control compared to control group.These observations prompted us to postulate the possible mechanism of antihyperglycemic action of the plants to be inhibition of digestion and absorption of dietary carbohydrates in the intestine, modulation of carbohydrate metabolizing enzymes, augmenting insulin synthesis and increasing peripheral utilization of glucose. These studies have provided adequate scientific evidence supporting the usage of these medicinal plants for the treatment of Type 2 Diabetes Mellitus in traditional system of medicines.

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Inhibitory activities of Ficus benghalensis bark against carbohydrate hydrolyzing enzymes - An in vitro study

Cited by 14 publications

References 12 publications

Health‐promoting bioactivity and in vivo genotoxicity evaluation of a hemiepiphyte fig, Ficus dubia

Health‐promoting bioactivity and in vivo genotoxicity evaluation of a hemiepiphyte fig, Ficus dubia

Evaluation of the inhibition of carbohydrate hydrolysing enzymes, antioxidant activity and polyphenolic content of extracts of ten African Ficus species (Moraceae) used traditionally to treat diabetes

Capillary Liquid Chromatographic Fingerprint for Identification and Authentication of from Its Related Species

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