Inhibitory Activity of Boswellic Acids from<i>Boswellia serrata</i>against Human Leukemia HL-60 Cells in Culture

Yu, Shanshan; Ho, Chi‐Tang; Chin, Chee‐Kok; Badmaev, Vladimir; Ma, Wei; Huang, Mou‐Tuan

doi:10.1055/s-2006-957444

Cited by 147 publications

(89 citation statements)

References 5 publications

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“…This is consistent with the findings that the acetyl derivative of boswellic acid {3-O-acetyl-11-keto-β-boswellic acid (AKBA)} from Boswellia serratia (Shao et al, 1998) and the 16-acetate derivative of gitoxigenin from Digitalis purpurea (Lopez-Lazaro et al, 2003) are also more active than the parent compounds.…”

Section: Discussionsupporting

confidence: 85%

Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells

Einbond

et al. 2008

Phytomedicine

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The purpose of this report is to explore the growth inhibitory effect of extracts and compounds from black cohosh and related Cimicifuga species on human breast cancer cells and to determine the nature of the active components. Black cohosh fractions enriched for triterpene glycosides and purified components from black cohosh and related Asian species were tested for growth inhibition of the ER − Her2 overexpressing human breast cancer cell line MDA-MB-453. Growth inhibitory activity was assayed using the Coulter Counter, MTT and colony formation assays.Results suggested that the growth inhibitory activity of black cohosh extracts appears to be related to their triterpene glycoside composition. The most potent Cimicifuga component tested was 25-acetyl-7,8-didehydrocimigenol 3-O-β-D-xylopyranoside, which has an acetyl group at position C-25. It had an IC 50 of 3.2 µg/ml (5 µM) compared to7.2 µg/ml (12.1 µM) for the parent compound 7,8-didehydrocimigenol 3-O-β-D-xylopyranoside. Thus, the acetyl group at position C-25 enhances growth inhibitory activity.The purified triterpene glycoside actein (β-D-xylopyranoside), with an IC 50 equal to 5.7 µg/ml (8.4 µM), exhibited activity comparable to cimigenol 3-O-β-D-xyloside. MCF7 (ER + Her2 low) cells transfected for Her2 are more sensitive than the parental MCF7 cells to the growth inhibitory effects of actein from black cohosh, indicating that Her2 plays a role in the action of actein. The effect of actein on Her2 overexpressing MDA-MB-453 and MCF7 (ER + Her2 low) human breast cancer cells was examined by fluorescent microscopy. Treatment with actein altered the distribution of actin filaments and induced apoptosis in these cells.These findings, coupled with our previous evidence that treatment with the triterpene glycoside actein induced a stress response and apoptosis in human breast cancer cells, suggest that compounds from Cimicifuga species may be useful in the prevention and treatment of human breast cancer.

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Section: Discussionsupporting

confidence: 85%

Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells

Einbond

et al. 2008

Phytomedicine

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“…However, besides its effect on MM cells, AKBA was also shown to suppress the growth of glioma, colon cancer, prostate and leukemic cells. These effects on multiple cancer targets can occur through the inhibition of the extracellular signal regulated kinase 1 and 2 (ERK1/2) phosphorylation, NF-jB pathway inhibition via IjB kinase (IKK) as well as topoisomerase I inhibition (Glaser et al 1999;Hoernlein et al 1999;Liu et al 2002;Park et al 2002;Shao et al 1998;Syrovets et al 2005 observations suggest that AKBA does not specifically affect STAT3 activity but has a wide field of action. Similarly, the main component isolated from the medicinal plant Nigella sativa, thymoquinone (TQ), was shown to inhibit both constitutive and IL-6-induced STAT3 phosphorylation.…”

Section: Multiple Myelomamentioning

confidence: 99%

Dietary compounds as potent inhibitors of the signal transducers and activators of transcription (STAT) 3 regulatory network

et al. 2012

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Signal transducers and activators of transcription (STAT) proteins were described as a family of latent cytosolic transcription factors whose activation is dependent on phosphorylation via growth factor-and cytokine-membrane receptors including interferon and interleukin, or by nonreceptor intracellular tyrosine kinases, including Src. A vast majority of natural substances are capable of modulating mitogenic signals, cell survival, apoptosis, cell cycle regulation, angiogenesis as well as processes involved in metastasis development. The inhibition of STAT3 phosphorylation by natural and dietary compounds leads to decreased protein expression of STAT3 targets essentially involved in regulation of the cell cycle and apoptotic cell death. This review details the cell signaling pathways involving STAT transcription factors as well as the corresponding compounds from nature able to interfere with this regulatory system in human cancer.The family of STAT transcription factors Structure and function STAT (signal transducers and activators of transcription) proteins were originally described as a family of cytoplasmic transcription factors. In mammals, seven members of this family, each consisting of 750-900 amino acids, have been identified: STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. These transcription factors act as dimers (homo-or hetero-dimers), and their activation is dependent on their phosphorylation or via membrane receptors stimulated by either extracellular factors, including interferon (IFN) and interleukin (IL-6), or by intracellular kinases independent of receptors, including Src. Activation of STATs is usually transient and highly regulated. STAT proteins contain seven conserved structural and functional domains ( First, the amino terminal NH 2 domain is involved in the dimerization of STAT proteins and in the stabilization of the interaction established between these dimers and DNA response elements (Braunstein et al. 2003;Mertens et al. 2006). The coiled-coil domain is responsible for controlling the process of import and export of proteins into and from the nucleus (Schindler et al. 2007). The domain that binds DNA, or the DBD (DNA binding domain), is involved in the physical interaction with STAT3-response elements in the promoters of target genes. With the exception of STAT2, all activated STAT homodimers bind directly to a palindromic sequence, the GAS (IFN-gammaactivated site), TTTCCNGGAAA (Becker et al. 1998). The ''linker'' domain localizes the active dimer to the DNA binding site. The transcriptional activation domain (TAD) contains sites of phosphorylation of serine residues that allow the recruitment of coactivators such as RNA polymerase II, histone acetyltransferase (HAT) (Paulson et al. 2002), histone deacetylase (HDAC) (Rascle et al. 2003) and chromatin modification complexes.Finally, two sites are particularly critical for the activity of STAT. These are the SH2 domain (Src homology 2, amino acids 575-680), which is linked to the DBD by the linker domain, and a conser...

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“…1-6) These acids have also been shown to possess potential chemopreventive effects, e.g., these inhibited growth of brain tumor 7) and meningioma cells, 8) and induced apoptosis in human leukemia 9) and hepatoma cells. 10) In view of the pharmacological importance of the natural compounds derived from Boswellia resin, 1,6) we were especially interested to undertake investigation on the constituents of Boswellia resin in order to evaluate their further pharmacological potentials.…”

Section: )mentioning

confidence: 99%

Cancer Chemopreventive Effects and Cytotoxic Activities of the Triterpene Acids from the Resin of Boswellia carteri

Akihisa

Tabata

Banno

et al. 2006

Biological & Pharmaceutical Bulletin

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The Boswellia species (Burseraceae), which are trees native to Ethiopia, Somalia, India, and the Arabic peninsula, produce a gum resin that is known as olibanum (frankincense). The resin of B. carteri and B. serrata has been used for the treatment of rheumatoid arthritis and other inflammatory diseases in the traditional medicine in many countries. 1)The search for the active principles of the resin resulted in the isolation of boswellic acids that belong to ursane-and oleanane-type pentacyclic triterpenes.1-6) These acids have also been shown to possess potential chemopreventive effects, e.g., these inhibited growth of brain tumor 7) and meningioma cells, 8) and induced apoptosis in human leukemia 9) and hepatoma cells. 10) In view of the pharmacological importance of the natural compounds derived from Boswellia resin, 1,6) we were especially interested to undertake investigation on the constituents of Boswellia resin in order to evaluate their further pharmacological potentials. In our recent paper, 11) we have reported the isolation and identification of fifteen triterpene acids (1-14, 16) and two diterpenes (17, 18) from the MeOH extract of the resin of B. carteri along with the evaluation of the inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice of compounds 1-14, 16-18, and 15 (the acetyl derivative of 14). This paper describes the inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) by TPA in Raji cells and on activation of (Ϯ)-(E)-methyl-2[(E)-hydroxyimino]-5-nitro-6-methoxy-3-hexemide (NOR 1), a nitrogen oxide (NO) donor, and cytotoxic activities against three human neuroblastoma cell lines, IMR-32, NB-39, and SK-N-SH in vitro for compounds 1-18. MATERIALS AND METHODS Materials and ChemicalsThe olibanum resin with certified botanical origin, Boswellia carteri Birdwood (Somalia), was purchased from Scents of Earth (Sun City, CA, U.S.A.; http://www.scents-of-earth.com).11) Chemicals were purchased as follows: TPA from ChemSyn Laboratories (Lenexa, KS, U.S.A.), b-carotene, glyzyrrhizin, and cisplatin (CDDP) from Sigma Chemical Co. (St. Louis, MO, U.S.A.), the EBV cell culture reagents and n-butanoic acid from Nacalai Tesque, Inc. (Kyoto, Japan), and NOR 1 and carboxy-PTIO from Dojindo Laboratories (Kumamoto, Japan). Three human neuroblastoma cell lines, IMR-32, NB-39, and SK-N-SH, were maintained in RPMI-1640 medium (Invitrogen) supplemented with 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% fetal bovine serum (FBS) (Invitrogen). All of them were maintained at 37°C/5% CO 2 in a humid environment.Extraction and Isolation Extraction of the resin of B. carteri and isolation of compounds 1-18 from the extract was described in our recent paper.11) In brief, the pulverized resin of B. carteri (10 g) was extracted with MeOH to yield extract (8.95 g). The extract was suspended in n-hexaneMeOH-H 2 O (19 : 19 : 2), giving n-hexane (5.05 g) and MeOH-H 2 O fractions. The latter fraction was partitioned in EtOAc-H 2 O (1 : 1, v/v), yielding E...

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Inhibitory Activity of Boswellic Acids fromBoswellia serrataagainst Human Leukemia HL-60 Cells in Culture

Cited by 147 publications

References 5 publications

Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells

Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells

Dietary compounds as potent inhibitors of the signal transducers and activators of transcription (STAT) 3 regulatory network

Cancer Chemopreventive Effects and Cytotoxic Activities of the Triterpene Acids from the Resin of Boswellia carteri

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