Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease

Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses

“…A phytochemical investigation of yellow rhizomes of this species from Thailand led to the isolation of six compounds, which are shown in Figure 1. These compounds were assayed against the DENV-2 NS3 protease [47]. The enzymatic assays were carried out using the fluorogenic peptide Boc-Gly-Arg-Arg-MCA, which is an active substrate of the DENV-2 NS3 protease.…”

An Overview on Cardamonin

“…3 Cardamonin's name comes from the fact that it can be found in cardamom spice (Fig. 4), but since the first article where it was described, 22 it has been found in many other (some of them edible) plant species, such as Alpinia blepharocalyx, 23,24 Alpinia gagnepainii, 25 Alpinia conchigera, 26,27 Alpinia hainanensis, [28][29][30][31][32][33][34][35][36][37][38][39][40] Alpinia malaccensis, 41 Alpinia mutica, 42,43 Alpinia pricei, 44 Alpinia rafflesiana, [45][46][47][48] Alpinia speciosa, 22,49,50 Amomum subulatum, 51 Artemisia absinthium, 52 Boesenbergia pandurata, [53][54][55][56][57][58][59][60] Boesenbergia rotunda, 61,62 Carya cathayensis, 63 Cedrelopsis grevei, 64 Combretum apiculatum, 65 Comptonia pere...…”

“…Posterior studies also showed, however, some inhibitor effects over the dengue virus type 2 (DV2) NS3 protease. 61 Dengue is still endemic over 100 countries and there is no vaccine available so far or even any specific treatment. No positive control was used in the study; inhibition was assumed using the LineweaverBurk plot, whereas cardamonin had a 71% and 39% inhibition rate with a 2 lM dengue-2 protease complex using concentration of 1480 and 444 lM, respectively.…”

Molecular Docking Studies of Selected Medicinal Drugs as Dengue Virus-2 Protease Inhibitors

“…The structural model of the protease, namely DH-1 (Heh et al 2013), was used as the target protein. Pinostrobin (1) and 4-hydroxypanduratin A (2) were utilized as standard ligands, each representing the non-competitive and competitive inhibitors of NS2B-NS3 protease of DENV-2, respectively (Frimayanti et al 2011;Heh et al 2013;Kiat et al 2006;Othman et al 2008). Therefore, this study aimed to investigate the binding interactions and search for the best orientation of ligandprotease complex with the lowest binding energy involving the selected antibiotics and NSAID.…”