Inhibitory Effect of 3-Cyanobenzoic Acid on Initial Growth of Maize Seedlings and Its Biochemical Impacts on Antioxidant and Energy Metabolisms

de Souza, Mariana Conceição; Constantin, Renato Polimeni; Abrahão, Josielle; de Paiva Foletto-Felipe, Marcela; Grizza, Luiz Henryque Escher; Constantin, Rodrigo Polimeni; dos Santos, Wanderley Dantas; Ferrarese-Filho, Osvaldo; Marchiosi, Rogério

doi:10.1007/s00344-023-11099-5

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…We chose soybean, a C3 dicot, and maize, a C4 monocot, as our model plants for comparing their metabolic responses to NQD exposure. Based on our previous studies, we determined that growing soybean and maize seedlings in a hydroponic system would be the most effective method to evaluate the molecules with biological activities [22][23][24][25]. This system provides optimal growth conditions, easy access for treatment, and minimizes the risk of compound adsorption to the soil or substrate, thereby maximizing the biological activity of the NQD.…”

Section: Introductionmentioning

confidence: 99%

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and Its Impact on Soybean and Maize Growth

Almeida,

Abrahão,

Seixas

et al. 2024

Agronomy

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The shikimate pathway is crucial for the biosynthesis of aromatic amino acids in plants and represents a promising target for developing new herbicides. This work aimed to identify inhibitors of shikimate dehydrogenase (SDH), a key enzyme of the shikimate pathway that catalyzes the conversion of 3-dehydroshikimate to shikimate. Virtual screening and molecular dynamic simulations were performed on the SDH active site of Arabidopsis thaliana (AtSDH), and 6-nitroquinazoline-2,4-diol (NQD) was identified as a potential inhibitor. In vitro assays showed that NQD decreased the activity of AtSDH by reducing Vmax while keeping KM unchanged, indicating non-competitive inhibition. In vivo, hydroponic experiments revealed that NQD reduced the root length of soybean and maize. Additionally, NQD increased the total protein content and certain amino acids. Soybean roots uptake NQD more efficiently than maize roots. Furthermore, NQD reduced shikimate accumulation in glyphosate-treated soybean roots, suggesting its potential to restrict the flow of metabolites along the shikimate pathway in soybean. The simultaneous treatment of maize seedlings with glyphosate and NQD accumulated gallic acid in the roots, indicating that NQD inhibits SDH in vivo. Overall, the data indicate that NQD inhibits SDH both in vitro and in vivo, providing valuable insights into the potential development of herbicides targeting SDH.

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Section: Introductionmentioning

confidence: 99%

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and Its Impact on Soybean and Maize Growth

Almeida,

Abrahão,

Seixas

et al. 2024

Agronomy

Self Cite

View full text Add to dashboard Cite

show abstract

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and its Impact on Soybean and Maize Growth

Almeida,

Abrahão,

Seixas

et al. 2024

Preprint

View full text Add to dashboard Cite

The shikimate pathway is crucial for the biosynthesis of aromatic amino acids in plants and represents a promising target for developing new herbicides. This work aimed to identify inhibitors of shikimate dehydrogenase (SDH), a key enzyme of the shikimate pathway that catalyzes the conversion of 3-dehydroshikimate to shikimate. Virtual screening and molecular docking were performed on the SDH active site of Arabidopsis thaliana (AtSDH), and 6-nitroquinazoline-2,4-diol (NQD) was identified as a potential inhibitor. In vitro assays showed that NQD decreased the activity of AtSDH by reducing Vmax while keeping KM unchanged, indicating noncompetitive inhibition. In vivo, hydroponic experiments revealed that NQD reduced the root length of soybean and maize. Additionally, NQD increased the total protein content and certain amino acids. Soybean roots uptake NQD more efficiently than maize roots. Furthermore, NQD reduced shikimate accumulation in glyphosate-treated soybean roots, suggesting its potential to restrict the flow of metabolites along the shikimate pathway in soybean. Simultaneous treatment of maize seedlings with glyphosate and NQD accumulated gallic acid in the roots, indicating that NQD inhibits SDH in vivo. Overall, the data indicate that NQD inhibits SDH both in vitro and in vivo, providing valuable insights into the potential development of herbicides targeting SDH.

show abstract

Inhibitory Effect of 3-Cyanobenzoic Acid on Initial Growth of Maize Seedlings and Its Biochemical Impacts on Antioxidant and Energy Metabolisms

Cited by 2 publications

References 63 publications

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and Its Impact on Soybean and Maize Growth

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and Its Impact on Soybean and Maize Growth

Unraveling Shikimate Dehydrogenase Inhibition by 6-Nitroquinazoline-2,4-diol and its Impact on Soybean and Maize Growth

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