Inhibitory effect of 4-ethylcatechol on β-glucuronidase activity

Kuji, Makoto; Itoh, Nanako; Ohba, Yui; Yamada, Kiyoshi; Hashimoto, Kei

doi:10.3136/fstr.27.797

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Maximum resistance to phenol was observed up to the range of 0.8% (7.1931 log CFU) after which it decreased drastically to 6.054 log CFU (Figure 4). A study by Kuji et al (2021) have observed Lactobacillus plantarum 299 and Lactobacillus reuteri DSM 17938 degrading phenolic acids via hydrolysis of phenols to either dihydrocaffeic acid or 4vinylcatechol and 4-ethylcatechol. In the contrary, some reports stated that Lactobacillus strains were only able to tolerate phenol concentration to 0.4% and the viability decreased by 50% at 0.6% (Hoque et al, 2010;De et al, 2017;Mannan et al, 2017;Reuben et al, 2020).…”

Section: Resistance Of Isolated Probiotic Bacteria To Phenol and Solv...mentioning

confidence: 99%

Characterization of Pediococcus pentosaceus strains isolated from Pickle and Moor Kuzhambu

Kurian¹,

Paari²

2023

MJM

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Aims: Consumption of fermented foods are known to provide various health benefits. The present study was aimed to isolate novel potent probiotic strains from the homemade fermented Indian recipe Moor Kuzhambu and Pickle and its characterization to elucidate the efficacy of isolate in cholesterol and heavy metal reduction. Methodology and results: Cultures isolated from Pickle (CK2) and Moor Kuzhambu (CK3) were identified as different strains of Pediococcus pentosaceus using the 16S rDNA sequence-based bacterial identification method. The study analysed the survival of probiotic strains under the influence of various chemical and natural stimulants. The isolated strains exhibited tolerance to gastric juice and were able to exhibit a broad range of tolerance to varying temperatures, pH, NaCl, solvent, phenol, trypsin and artificial gastric juice. Microencapsulation studies were conducted using alginate and chitosan to increase the shelf life of the isolated probiotic. Preliminary analysis regarding cell surface studies such as autoaggregation, co-aggregations and cell surface hydrophobicity determined the ability of the strains to aggregate on to intestinal cell surface and manifest competitive pathogen displacement. Conclusion, significance and impact of study: Remarkable biofilm reduction of 48% to 80% was observed in the probiotic-supplemented samples. Similarly, a reduction of 80% to 85% free cholesterol was noted in cholesterol assimilation assays and heavy metal (Cu + , Pb + , Zn + and Fe + ) assimilation ability was observed. Further studies are required to characterize the nature of the secretory products and their mode of action in the survival and immune enhancement in animal models.

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Section: Resistance Of Isolated Probiotic Bacteria To Phenol and Solv...mentioning

confidence: 99%

Characterization of Pediococcus pentosaceus strains isolated from Pickle and Moor Kuzhambu

Kurian¹,

Paari²

2023

MJM

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“…The microbial production in the gut of ethylphenols has also attracted attention related to the host health, as the host-sulfated derivative of 4-ethylphenol, the 4-ethylphenyl sulphate, has been demonstrated to be a neuroactive microbial molecule that impacts brain activity and complex behaviors in animals [ 30 ]. In addition, the 4-ethylcatechol has been proposed to contribute to reducing the risk of colon cancer [ 32 ]. The precursor of ethylphenol, vinylphenol, is also known to influence behavior in plants [ 33 ], which previously raised the question of whether vinyl derivatives of HCAs can also impact behavior in animals [ 8 ].…”

Section: Reduction Of Vinyl Derivatives Of Hydroxycinnamic Acids Into...mentioning

confidence: 99%

Revised Aspects into the Molecular Bases of Hydroxycinnamic Acid Metabolism in Lactobacilli

Felipe

2023

Antioxidants

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Hydroxycinnamic acids (HCAs) are phenolic compounds produced by the secondary metabolism of edible plants and are the most abundant phenolic acids in our diet. The antimicrobial capacity of HCAs is an important function attributed to these phenolic acids in the defense of plants against microbiological threats, and bacteria have developed diverse mechanisms to counter the antimicrobial stress imposed by these compounds, including their metabolism into different microbial derivatives. The metabolism of HCAs has been intensively studied in Lactobacillus spp., as the metabolic transformation of HCAs by these bacteria contributes to the biological activity of these acids in plant and human habitats or to improve the nutritional quality of fermented foods. The main mechanisms known to date used by Lactobacillus spp. to metabolize HCAs are enzymatic decarboxylation and/or reduction. Here, recent advances in the knowledge regarding the enzymes that contribute to these two enzymatic conversions, the genes involved, their regulation and the physiological significance to lactobacilli are reviewed and critically discussed.

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Mechanisms of gut bacterial metabolism of dietary polyphenols into bioactive compounds

Alqudah,

Claesen

2024

Gut Microbes

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The fruits and vegetables we consume as part of our diet are rich in bioactive metabolites that can prevent and ameliorate cardiometabolic diseases, cancers, and neurological conditions. Polyphenols are a major metabolite family that has been intensively investigated in this context. However, for these compounds to exert their optimal bioactivity, they rely on the enzymatic capacity of an individual’s gut microbiota. Indeed, for most polyphenols, the human host is restricted to more basic metabolism such as deglycosylation and hepatic conjugation. In this review, we discuss the mechanisms by which gut bacteria metabolize the core scaffold of polyphenol substrates, and how their conversion into bioactive small molecules impacts host health.

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Inhibitory effect of 4-ethylcatechol on β-glucuronidase activity

Cited by 3 publications

References 43 publications

Characterization of Pediococcus pentosaceus strains isolated from Pickle and Moor Kuzhambu

Characterization of Pediococcus pentosaceus strains isolated from Pickle and Moor Kuzhambu

Revised Aspects into the Molecular Bases of Hydroxycinnamic Acid Metabolism in Lactobacilli

Mechanisms of gut bacterial metabolism of dietary polyphenols into bioactive compounds

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