Inhibitory effect of a Korean traditional medicine, Honghwain–Jahage (water extracts of Carthamus tinctorius L. seed and Hominis placenta) on interleukin-1-mediated bone resorption

cAMP-PKA signaling pathway regulates bone resorption mediated by processing of cathepsin K in cultured mouse osteoclasts

“…WT, an PI3-kinase inhibitor, has been previously shown to inhibit a number of osteoclast functions, including cell attachment and spreading [38], ruffled border formation [30], and bone resorption both in vitro and in vivo [36]. WT inhibited in a dose dependent manner Cat K processing in mouse osteoclasts, and eventually resulted in the secretion of unprocessed pro Cat K into the culture media [24].…”

“…MOUSE long bone preparation was also described in our paper [30]. The method for disaggregating osteoclasts from fresh long bone and their subsequent use for in vitro assays has also been described in detail [31,32].…”

Herbal formulation, Yukmi-jihang-tang-Jahage, regulates bone resorption by inhibition of phosphorylation mediated by tyrosine kinase Src and cyclooxygenase expression

“…A literature survey on the YJ revealed that there is no scientific evidence of its usefulness in the treatment of RA and osteoporosis. The need for safer and effective anti-inflammatory drug and the lack of enough scientific data to support the claims made in ancient literature prompted the present study (Hong et al, 2002;Yuk et al, 2002). Thus, it still occupies an important place in traditional Oriental medicine.…”

PGE2 induces IL-1β gene expression in mouse osteoblasts through a cAMP–PKA signaling pathway

“…Mouse calvarial osteoblasts were isolated from neonatal BALBc mice by enzymatic digestion, as described previously [17,18]. The population released during the last three digestions was highly enriched in cells expressing two markers of the osteoblast lineage, namely alkaline phosphatase and osteocalcin [19].…”