Inhibitory Effect of Acetylshikonin on the Activation of NADPH Oxidase in Polymorphonuclear Leukocytes in Both Whole Cell and Cell-Free Systems.

Kawakami, Naoko; Koyama, Yasuo; Tanaka, Jyunya; Ohara, Akira; Hayakawa, Tetsuo; Fujimoto, Sadaki

doi:10.1248/bpb.19.1266

Cited by 15 publications

(18 citation statements)

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“…It did not inhibit previously induced NADPH oxidase activity. These results suggest that acetylshikonin inhibits the formation of the NADPH oxidase complex needed for the activation of the respiratory burst in PMNs, but does not directly inhibit the activity of NADPH oxidase (Kawakami et al, 1996).…”

Section: Inhibition Of Leukotriene B4 Biosynthesis (Ltb4)mentioning

confidence: 80%

Cellular pharmacology studies of shikonin derivatives

Chen

Yang

Oppenheim

et al. 2002

Phytotherapy Research

371

274

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The naphthoquinone pigment, shikonin, isolated from Lithospermum erythrorhizon Sieb. et Zucc.(Boraginaceae) and its derivatives are the active components isolated from the Chinese herbal therapeutic, Zicao. Historically, Zicao root extracts have been used to treat macular eruption, measles, sore-throat, carbuncles and burns. Multiple pharmacological actions have been attributed to shikonin, e.g. antiinflammatory, antigonadotropic and anti-HIV-1 activity. In this review, several therapeutic applications of shikonin will be summarized including its pleiotropic, antiinflammatory and antitumour effects. Widely diverse and sometimes conflicting activities have been attributed to shikonin, e.g. wound healing, enhanced granuloma formation, suppression of local acute inflammatory reactions, inhibition of angiogenesis, inhibition of select chemokine ligands, inhibition of DNA topoisomerase activity, inhibition of platelet activation and antimicrobial activity. Comparison of the various reported mechanisms of action for shikonin lead us to hypothesize that shikonin is an effective inhibitor of protein-protein interaction with multiple targets in both the intracellular and extracellular compartments. This general inhibitory effect can account for the broad spectrum of shikonin biological and pharmacological activities.

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Section: Inhibition Of Leukotriene B4 Biosynthesis (Ltb4)mentioning

confidence: 80%

Cellular pharmacology studies of shikonin derivatives

Chen

Yang

Oppenheim

et al. 2002

Phytotherapy Research

371

274

View full text Add to dashboard Cite

show abstract

“…•− production from phorbol-12-myristate-13-acetate (PMA) stimulated human neutrophils This assay was performed as described by Kawakami et al (1996) and Tan and Berridge (2000) with slight modifications. Human neutrophils (1 × 10 4 neutrophils/mL) were preincubated for 5 min at 37 • C, followed by the addition of plant extracts (1-25 g/mL) or diphenyleneiodinium (10 M) or superoxide dismutase (20 g/mL) or staurosporin (0.1 g/mL) to a final volume of 250 L. The control reaction contained buffer only.…”

Section: Omentioning

confidence: 99%

“…In another set of experiment, diphenyleneiodinium (10 M) or staurosporin (0.1 g/mL) or n-hexane and ethyl acetate extracts (10 g/mL) and methanol extract (25 g/mL) were added to the neutrophils 3 min after stimulation with PMA (Kawakami et al, 1996). Otherwise, the entire experiment was performed as described above.…”

Section: Omentioning

confidence: 99%

A study on antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective actions of Aegiceras corniculatum (stem) extracts

Roome

Dar

Ali

et al. 2008

Journal of Ethnopharmacology

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“…Chen et al showed another possibility, that shikonin blocks chemokine binding to CC chemokine receptor-1 [11]. Other effects that might contribute for the anti-inflammatory activity of shikonins are the prevention of leukotriene B4 biosynthesis in leukocytes [12], inhibition of superoxide-generating activity [13,14] and impairment of phosphatidylinositol signaling in neutrophils [15]. Acetylshikonin was found to suppress histamine release from rat peritoneal mast cells stimulated with compound 48/80 [8], a classical mast cell secretagogue which directly activates GTP-binding protein and induces phospholipase C-related cell signaling [16].…”

Section: Introductionmentioning

confidence: 99%