Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo

Abstract

AIM:To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism. METHODS:MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation o f S G C-7 9 0 1 c e l l s . A p o p t o s i s -i n d u c i n g e f f e c t was determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling with Hoechst staining. Expression of mRNA and protein in Bcl-2 and Bax was analyzed by reverse transcription-polymerase chain reaction and Western blot. Antitumor effect of acetylshikonin on a mouse SGC-7901 model was also determined. RESULTS:

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

“…It has been reported earlier that shikonin possesses tumour cell-killing activity against a variety of cell lines (Min et al 2008;Wu et al 2004;Zeng et al 2009). Also ACS demonstrated antitumour effects in malignant cell lines (Xiong et al 2009).…”

The anti-proliferation effect of Aikete injection on hepatocellular carcinoma in vitro and in vivo

“…In recent studies, shikonin and its derivatives were demonstrated to induce apoptosis of tumor cells. It has been reported that shikonin could induce A375-S2 cells apoptosis and cell cycle arrest through caspase-9 dependent pathways (Wu et al, 2004a); acetylshikonin induced SGC-7901 cells apoptosis by downregulating Bcl-2 expression and upregulating Bax expression (Zeng et al, 2009); shikonin induced apoptosis in HeLa cells via caspase-3 activation, blockage of DNA synthesis, and cell cycle arrest (Wu et al, 2004b); shikonin could induce apoptosis in COLO 205 cells via modulating the Bcl-2 family, upregulating p27, p53, releasing cytochrome c, and sequentially activating caspases (Hsu et al, 2004).…”

“…Aikete injection is composed of acetylshikonin and β,β-dimethylacrylshikonin (Figure 1), the two main effective anti-tumor components of shikonin (Hu et al, 2006;Kundakovic et al, 2006;Zeng et al, 2009). Although the anti-tumor effect of acetylshikonin and β,β-dimethylacrylshikonin has been reported widely, little is known about the effects of the combination of the two components on cancer cells.…”

Activation of JNK/p38 Pathway is Responsible for α-Methyl-n-butylshikonin Induced Mitochondria-Dependent Apoptosis in SW620 Human Colorectal Cancer Cells

“…Hsu PC et al demonstrated that shikonin induced apoptosis through up-regualtion of p27, p53, Bax and activation of caspases in human colorectal carcinoma cells (Hsu et al, 2004). Zeng Y et al also found that acetylshikonin led to cell apoptosis in human gastric carcinoma cell line SGC-7901 via elevation of Bax /Bcl-2 ratio (Zeng et al, 2009). Recently, we reported that β,β-dimethylacrylshikonin induced mitochondria-dependent apoptosis via ERK1/2 pathway in human gastric cancer SGC-7901 cells (Shen et al, 2012).…”