Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria

Abstract

BackgroundHerbs and spices are very important and useful as therapeutic agent against many pathological infections. Increasing multidrug resistance of pathogens forces to find alternative compounds for treatment of infectious diseases.MethodsIn the present study the antimicrobial potency of garlic and ginger has been investigated against eight local clinical bacterial isolates. Three types of extracts of each garlic and ginger including aqueous extract, methanol extract and ethanol extract had been assayed separately against drug resistant Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Shigella sonnei, Staphylococcusepidermidis and Salmonella typhi. The antibacterial activity was determined by disc diffusion method.ResultsAll tested bacterial strains were most susceptible to the garlic aqueous extract and showed poor susceptibility to the ginger aqueous extract. The (minimum inhibitory concentration) MIC of different bacterial species varied from 0.05 mg/ml to 1.0 mg/ml.ConclusionIn the light of several socioeconomic factors of Pakistan mainly poverty and poor hygienic condition, present study encourages the use of spices as alternative or supplementary medicine to reduce the burden of high cost, side effects and progressively increasing drug resistance of pathogens.

Different Extracts of Zingiber officinale Decrease Enterococcus faecalis Infection in Galleria mellonella

“…To the best of our knowledge, this is the first study on extracts of plants to treat microbial infections in G. mellonella model, consequently there are no studies with similar methodology in order to compare and better discuss the present findings. Even so, these results agree with most published reports regarding the in vitro antimicrobial activity of ginger (19,20).…”

In-vitro effects of garlic extracts on pathogenic fungi Botrytis cinerea, Penicillium expansum and Neofabraea alba

“…Aqueous dilutions of the garlic extract showed better activity than the ethanol dilutions of the extract. Previous studies both support 28 and contradict 29 this finding; however, all studies do support the fact that aqueous extract preparations do result in antimicrobial activity. When comparing aqueous preparations of extracts to preparations using other organic solvents tested against B. cinerea, a parallel may be drawn between results from the present study and those achieved by Senhaji et al 27 , in that the present study revealed that complete (100%) mycelial inhibition could be achieved by using a 60% aqueous diluted extract (Table 1).…”

Antibacterial Efficacy of Aqueous Plant Extracts against Storage Soft Rot of Potato Caused by Erwinia carotovora

“…Hydrophilic leaf extracts of Juglans regia, bark of Salix albawasfound effective against E. coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus and Salmonella enteritis (Pop et al, 2013;Shah et al, 2013 Askari et al, 2013;Haouat et al, 2013;Das et al, 2012;Ayaz et al, 2016;Monika et al, 2014). Gull et al, (2012) Effect of aqueous plant extracts on incidence and severity of soft rot disease caused by Erwinia carotovora on stored potato tubers Among 13 plants tested in-vitro against the growth of Erwinia carotovora, three plant extracts showing highest efficacy with respect to growth inhibition are selected for this experiment. Best results were obtained when treatments were given 12 hours prior to inoculation or simultaneously with the inoculation of the pathogen than when treatments were given 12 hours after the inoculation of the pathogen ( Fig.…”