Inhibitory effect of allyl and benzyl isothiocyanates on ochratoxin a producing fungi in grape and maize

Yang, Bolei; Li, Li; Geng, Hairong; Zhang, Chenxi; Wang, Gang; Yang, Shuo; Gao, Sheng; Zhao, Yang; Xing, Fuguo

doi:10.1016/j.fm.2021.103865

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…The spore germination and fungal growth of Aspergillus ochraceus , Aspergillus carbonarium , and Aspergillus niger were significantly inhibited when the concentration of A‐ITC was higher than 1.25 μg/mL. A‐ITC could significantly down‐regulate the expression of all five Ochratoxin A biosynthesis genes in A. niger and A. carbonarius 9 . A‐ITC causes rapid inhibition of Fusarium solani after 5 min, hyphal deformity, and electrolyte leakage.…”

Section: Introductionmentioning

confidence: 98%

“…A-ITC could significantly down-regulate the expression of all five Ochratoxin A biosynthesis genes in A. niger and A. carbonarius. 9 A-ITC causes rapid inhibition of Fusarium solani after 5 min, hyphal deformity, and electrolyte leakage. Loss of FsYvc1 (a yeast-like vacuolar transient receptor potential channel regulator) resulted in hypersensitivity of F. solani to A-ITC and induced reactive oxygen species (ROS) accumulation.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Jia

Zhao

et al. 2022

Pest Management Science

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Background Isothiocyanates (ITCs) generated from the ‘glucosinolates‐myrosinase’ defense system in the Brassicaceae exhibit broad antagonistic activity to various fungal pathogens. Nevertheless, the antifungal activity of ITCs to non‐adapted fungi of Brassicaceae plants were seldom determined. The inhibitory effects of ITCs on Cochliobolus heterostrophus were evaluated and the antagonistic mechanism was explored. Results The mycelium growth of C. heterostrophus was hindered significantly by allyl, 4‐(methylthio)‐butyl, and phenyethyl ITCs, 4MTB‐ITC exhibited the highest inhibitory effect on mycelium growth with an IC50 value of 53.4 μmol L−1. In addition, ITCs exhibited obvious inhibitory effect on conidia germination and pathogenicity of C. heterostrophus. Proteomic analysis indicated that the inhibition of C. heterostrophus by A‐ITC downregulated the expression of genes related to energy metabolism, oxidoreductase activity, melanin biosynthesis, and cell wall‐degrading enzymes. Furthermore, mutants ΔChtrx2 and ΔChnox1 showed increased sensitivity to ITCs, and melanin biosynthesis was inhibited significantly in C. heterostrophus in response to A‐ITC. Interestingly, unlike other pathogens that infected Brassicaceae plants, the SaxA in C. heterostrophus displayed no function in ITC degradation. In addition, the ITCs also exhibited obvious inhibitory effect on mycelium growth of Setosphaeria turcica, Fusarium graminearum, and Magnaporthe oryzae. Conclusion This study indicated that non‐Brassicaceae‐adapted pathogens are more sensitive to ITCs, and ITCs could have applications in protecting non‐Brassicaceae crops in future. In addition, loss of ChNOX1 and ChTRX2 increased the sensitivity of C. heterostrophus to ITCs. Our results provided potential utilization of ITCs to control diseases caused by non‐Brassicaceae pathogenic fungi. © 2022 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Jia

Zhao

et al. 2022

Pest Management Science

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“…Nevertheless, our analysis also revealed that compounds involved in defence mechanisms are also affected by the transition to selfing. Allyl isothiocyanate is a toxic compound also acting as a repellent against various organisms, including bacteria, fungi and herbivores (Agrawal and Kurashige, 2003; Clay et al ., 2009; Müller et al ., 2018; Yang et al ., 2021; Singh et al ., 2022). Strikingly, its emission is also reduced in the two selfers suggesting that the reproduction optimization in animal pollinated plants relies on a delicate balance between attraction and defence mechanism.…”

Section: Discussionmentioning

confidence: 99%

Convergence and molecular evolution of floral fragrance after independent transitions to self–fertilization

Woźniak¹,

Sartori

Kappel³

et al. 2022

Preprint

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The study of the independent evolution of similar characters can highlight important ecological and genetic factors that drive phenotypic evolution. The transition from reproduction by outcrossing to self-fertilization has occurred frequently throughout plant evolution. A common trend in this transition is the reduction of flower features in the selfing lineages, including display size, flower signals and pollinators' rewards. These changes are believed to evolve because resources invested in building attractive flowers are reallocated to other fitness functions as the pressures to attract pollinators decrease. We investigated the similarities in the evolution of flower fragrance after independent transitions to self-fertilization in Capsella. We identified a large number of compounds that are similarly changed in different selfer lineages, such that the composition of the flower scent can predict the mating system in this genus. We further demonstrate that the emission of some of these compounds convergently evolved based on mutations in different genes. In one of the Capsella selfing lineages, the loss of β-ocimene emission was caused by a mutation altering subcellular localization of the ortholog of TERPENE SYNTHASE 2 without apparent effects on its biosynthetic activity. This mutation appears to have been selected at the early stage of this selfing lineage establishment through the capture of a variant segregating in the ancestral outcrossing population. The large extent of convergence in the independent evolution of flower scent, together with the evolutionary history and molecular consequences of a causal mutation, suggest that the emission of specific volatiles has important fitness consequences in self-fertilizing plants without obvious energetic benefits.

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“…ITCs can act as poisons or deterrents against bacteria 11 , fungi 12 and herbivores 13 , 14 . Allyl isothiocyanate (AITC) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Appraisal of growth inhibitory, biochemical and genotoxic effects of Allyl Isothiocyanate on different developmental stages of Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)

Singh

Diksha

Mahajan

et al. 2022

Sci Rep

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Allyl isothiocyanate (AITC), a glucosinolates’ hydrolytic product, was studied for its anti-insect potential against an economically important, destructive tephritid pest, Zeugodacus cucurbitae (Coquillett). The first, second and third instar maggots of the pest were fed on artificial diets amended with varied concentrations of AITC viz. 5 ppm, 25 ppm, 50 ppm, 100 ppm, 150 ppm and 200 ppm with DMSO (0.5%) as control. Results revealed high larval mortality, alteration of larval period, prolongation of pupal and total developmental periods in all instars of the maggots treated with AITC as compared to controls. Percent pupation and percent adult emergence decreased in all larval instars. Growth indices viz. Larval Growth Index (LGI) and Total Growth Index (TGI) were negatively affected. Anti-nutritional/post ingestive toxicity of AITC was also revealed by the decrease in Food Assimilation (FA) and Mean Relative Growth rate (MRGR) values with respect to control. Profiles of PO (Phenol oxidase) and other detoxifying enzymes including SOD (Superoxide dismutases), CAT (Catalases), GST (Glutathione-S-transferases), EST (Esterases), AKP (Alkaline phosphatases) and ACP (Acid phosphatases) were also significantly influenced. The genotoxic effect of AITC was also evaluated by conducting comet assays at LC30 and LC50. Significant DNA damage in hemocytes was reflected by increase in Tail length (μm), Percent Tail DNA, Tail Moment (TM) and Olive Tail Moment (OTM) as compared to controls. The results indicated high potential of AITC as biopesticide for pest management.

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Inhibitory effect of allyl and benzyl isothiocyanates on ochratoxin a producing fungi in grape and maize

Cited by 19 publications

References 32 publications

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Antifungal mechanism of isothiocyanates against Cochliobolus heterostrophus

Convergence and molecular evolution of floral fragrance after independent transitions to self–fertilization

Appraisal of growth inhibitory, biochemical and genotoxic effects of Allyl Isothiocyanate on different developmental stages of Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae)

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