Inhibitory effect of ammonium ion on recovery of cryopreserved rice cells

Kuriyama, Akira; Watanabe, Kenji; Ueno, Saburo; Mitsuda, Hisateru

doi:10.1016/0168-9452(89)90028-9

Cited by 40 publications

(16 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The regrowth medium can be transitorily modified by incorporating compounds with osmotical properties, so as to reduce osmotic shock (Maddox et al, 1982-83). The mineral composition of the medium can be altered: in the case of rice cells, Kuriyama et al (1989) showed that ammonium ions had an inhibitory effect on the regrowth of cryopreserved rice cells. Their suppression during the first subcultures led to an improvement of regrowth.…”

Section: Cell Suspensionsmentioning

confidence: 99%

In vitro conservation of tropical plant germplasm — a review

Engelmann

1991

Euphytica

179

View full text Add to dashboard Cite

In vitro medium term conservation of tropical plant germplasm is used routinely in many laboratories. Growth reduction is achieved by modifying various parameters, such as temperature, culture medium, gaseous environment. For long term conservation, cryopreservation (i.e. storage in liquid nitrogen, -196 ° C) is the only current method available. Each successive step of the process requires precise conditions which have to be defined for each material. Cryopreservation protocols have been set up for more than 40 tropical species. Results obtained with various culture systems such as cell suspensions, protoplasts, calluses, meristems and embryos are discussed. The first example of the large scale application of cryopreservation (oil palm somatic embryos) is presented.

show abstract

Section: Cell Suspensionsmentioning

confidence: 99%

In vitro conservation of tropical plant germplasm — a review

Engelmann

1991

Euphytica

179

View full text Add to dashboard Cite

show abstract

“…The viability of cooled samples, following culture on ammonium-free MS medium for 5 days, was significantly increased compared to those cultured on MS medium. As shown in (Kuriyama et al, 1989;Kuriyama et al, 1996). Altering the recovery medium for the encapsulation-vitrification method improved recovery of the cryopreserved sweet potato shoot tips.…”

Section: Improvement In Recovery By Altering the Post-warming Mediummentioning

confidence: 64%

Cryopreservation of in Vitro Grown Shoot Tips of Sweet Potato (Ipomoea batatas L.) by the Encapsulation-Vitrification Method

Yi¹,

Lee²,

Lee³

et al. 2016

Korean Journal of Plant Resources

View full text Add to dashboard Cite

-Sweet potato (Ipomoea batatas L.) shoot tips grown in vitro were successfully cryopreserved by encapsulationvitrification. Encapsulated explants are very easily manipulated, due to the relatively large size of the alginate beads, and a large number of samples can be treated simultaneously. In this study, the effects of sucrose preculture, cryoprotectant preculture, and post-warm recovery media on regrowth, following liquid nitrogen (LN) exposure, were investigated to establish an efficient encapsulation-vitrification protocol for sweet potato. Shoot tips of plants grown in vitro were precultured in 0.3 M sucrose for 2 d before encapsulation. Encapsulated shoot tips were pre-incubated in liquid MS (Murashige and Skoog) medium containing 0.5 M sucrose for 16 h, before preculturing in sucrose-enriched medium (0.7 M sucrose) for 8 h. Shoot tips were osmoprotected with 35% plant vitrification solution 3 (PVS3) for 3 h, before being dehydrated with PVS3 for 2 h at 25°C. The encapsulated and dehydrated shoot tips were transferred to 2 mL cryotubes, suspended in 0.5 mL PVS3, and plunged directly into liquid N. High levels of shoot formation were obtained for the cv. Yeulmi (65.7%) and Yeonwhangmi (80.3%). The regrowth rates of cryopreserved samples in Yeulmi (78.9%) and Yeonwhangmi (91.3%), following culture on ammonium-free MS medium for 5 d, were much higher than those cultured on standard MS medium (65.7% and 80.3%, respectively). This encapsulation-vitrification is a promising method for the long-term preservation of sweet potato.

show abstract

“…After blotting the surface solution, the shoot tips were transferred onto 0.8% agar MS medium excluding NH4NO 3 (Kuriyama et al 1990) and cultured under standard conditions described previously.…”

Section: Viability and Regrowthmentioning

confidence: 99%

Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification

Niino

Sakai²,

Yakuwa

et al. 1992

Plant Cell Tiss Organ Cult

120

View full text Add to dashboard Cite

In vitro-grown shoot tips of apples (Malus domestica Borkh. cv. Fuji) were successfully cryopreserved by vitrification. Three-week-old in vitro apple plantlets were cold-hardened at 5°C for 3 weeks. Excised shoot tips from hardened plantlets were precultured on a solidified Murashige & Skoog agar medium (MS) supplemented with 0.7M sucrose for 1 day at 5°C. Following preculture shoot tips were transferred to a 2 ml plastic cryotube and a highly concentrated cryoprotective solution (designated PVS2) was then added at 25°C. The PVS2 contains (W/V) 30% glycerol, 15% ethylene glycol and 15% dimethylsulfoxide in medium containing 0.4 M sucrose. After dehydration at 25°C for 80 min, the shoot tips were directly plunged into liquid nitrogen. After rapid warming, the shoot tips were expelled into 2 ml of MS medium containing 1.2 M sucrose and then plated on agar MS medium. Direct shoot elongation was observed in approximately 3 weeks. The average rate of shoot formation was about 80%. This vitrification method was successfully applied to five apple species or cultivars and eight pear cultivars. This method appears to be a promising technique for cryopreserving shoot tips from in vitro-grown plantlets of fruit trees.

show abstract

Inhibitory effect of ammonium ion on recovery of cryopreserved rice cells

Cited by 40 publications

References 10 publications

In vitro conservation of tropical plant germplasm — a review

In vitro conservation of tropical plant germplasm — a review

Cryopreservation of in Vitro Grown Shoot Tips of Sweet Potato (Ipomoea batatas L.) by the Encapsulation-Vitrification Method

Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification

Contact Info

Product

Resources

About