Inhibitory effect of an aqueous extract of Radix Paeoniae Alba on calcium oxalate nephrolithiasis in a rat model

Abstract

Objective: To examine the effect of an aqueous extract of Radix Paeoniae Alba (RPA) on the formation of calcium oxalate (CaOx) stones and the potential mechanism underlying the effect.Materials and methods: An in vitro assay was used to determine whether the RPA extract prevents the formation of CaOx or promotes CaOx dissolution. We also investigated the efficacy of the extract in vivo as a preventive and therapeutic agent for experimentally induced CaOx nephrolithiasis in rats. Various biochemical, molecular, and histological parameters were assessed in kidney tissue and urine at the end of the in vivo experiment.Results: Significant dissolution of formed crystals (8.99 ± 1.43) and inhibition of crystal formation (2.55 ± 0.21) were observed in vitro after treatment with 64 mg/mL of the RPA extract compared with a control treatment (55.10 ± 4.98 and 54.57 ± 5.84, respectively) (p < .05). In preventive protocols, the RPA extract significantly reduced urinary and renal oxalate levels and increased urinary calcium and citrate levels compared to the control. In addition, the RPA preventive protocol significantly decreased osteopontin expression, renal crystallization, and pathological changes compared to the control. These changes were not observed in rats on the therapeutic protocol.Conclusions: RPA is a useful agent that prevents the formation of CaOx kidney stones.

Plant Extracts in Hyperoxaluria Treatment: A Review of Experimental and Clinical Research

“…In vivo animals Reducing oxalate concentration in rats' blood; S. Bouanani (2010) [31] nephroprotective Pomegranate juice Clinical Decrease of serum paraoxonasearylesterase activity C.R. Tracy (2014) [55] and supersaturation of calcium oxalate Punica granatum in vitro Inhibition of oxalate crystallization R. Kachkoul (2018) [32]…”

Dissipation Behavior of Three Fungicides during the Industrial Processing of Paeoniae Radix Alba and Associated Processing Factors

“…Paeoniae Radix Alba (PRA) is a common CHM that has been shown to have sedative, analgesic, anti-inflammatory, liver-protecting, and immune-regulating effects along with the ability to inhibit platelet aggregation [12]. As reported in the Chinese Pharmacopoeia, PRA is produced from the peony root that has been peeled and boiled.…”

Evaluation of antiurolithiatic effects of Parmelia perlata against calcium oxalate calculi in hyperoxaluric rats

“…mono and di-hydrates of CaOx (Aggarwal et al, 2013;Goyal et al, 2017;Khan, 1997). Urinary stone formation occurs as a result of cascade of physiochemical events started with supersaturation of urine and followed by nucleation, growth, aggregation and retention of crystals (Li et al, 2017;Moe, 2006). Despite of recognition of urolithiasis since stone-age and tremendous advances in the field of urology, in modern system of medicines, except few alkalizers and diuretics, there is no clinically satisfactory medicine that can either dissolve and/or prevent the formation or recurrence of urinary stones (Goyal et al, 2017).…”

“…Such techniques cannot hamper, even facilitate, the chances of stone recurrence and have some serious side effects like haemorrhage, renal fibrosis, infections, etc. (Li et al, 2017). Therefore, after adopting these treatment procedures, the patients have to be subjected to careful follow up for long time, and moreover these procedures are prohibitively costly for a common man.…”