Inhibitory effect of an ethanol extract mixture of Aralia elata leaf, Chaenomeles sinensis fruit and Glycyrrhizae radix on Aβ (25–35)-induced memory impairment

Abstract

The young shoots of Aralia elata, Chaenomeles sinensis fruit and Glycyrrhizae radix are edible and traditionally used as anti-inflammatory and antioxidant agents. The present study was performed to investigate the protective effect of an ethanol extract mixture of these three medicinal plants (ACG) against amyloid β protein (Aβ) (25-35)-induced memory impairment in an ICR mouse model. Memory impairment was induced by intracerebroventricular microinjection of 15 nmol Aβ (25-35) and assessed using the passive avoidance test and the Morris water maze test. The step-through latency in the passive avoidance test was decreased and the latency to reach the hidden platform in the Morris water maze test was increased in mice treated with Aβ (25-35), indicating memory impairment. This memory impairment induced by Aβ (25-35) was significantly prevented by chronic treatment with ACG (10, 25, and 50 mg/kg, p.o., 8 days). In memory impaired mice brain, cholinesterase activity and concentration of thiobarbituric acid reactive substance, a lipid peroxidation marker, were increased and glutathione level was decreased. These biochemical changes in Aβ (25-35)-treated mice were reversed by chronic administration of ACG. The present results suggest that antioxidant and anti-cholinesterase activities of ACG might be responsible for the inhibition of Aβ (25-35)-induced memory impairment and that ACG preparation may have a therapeutic role in preventing the progression of Alzheimer's disease.

Protective effect of an ethanol extract mixture of Aralia elata, Chaenomeles sinensis fruit, and Glycyrrhizae radix against cerebral ischemia-reperfusion injury in rats and excitotoxic and oxidative neuronal damage

“…Since three medicinal plants of A. elata, C. sinensis fruit, and Glycyrrhizae radix exhibit antioxidant and anti-inflammatory activities, it was hypothesized that the combination of these three plants might protect neurons against neurodegenerative diseases such as Alzheimer disease and stroke. In a previous report, an ethanolic extract of a mixture of A. elata, C. sinensis fruit, and Glycyrrhizae radix, which was named as ACG, inhibited Aβ (25-35)-induced memory impairment in mice [19]. The current study was conducted to further verify the neuroprotective effect of ACG on cerebral ischemic injury and excitotoxicity using both in vivo and in vitro studies.…”

Comparison of postharvest quality of Aralia elata shoot according to packaging methods

“…The major changes during the senescence of A. elata include loss in weight and change in color due to Korean J Food Preserv,30(4) (2023) dehydration and chlorophyll degradation, respectively, which consequently results in a reduction in their marketability. Recent studies have analyzed the secondary metabolites in A. elata and their bioactivity (Lee and Lee, 2021;Seong, 2021;Wei et al, 2021); however, information is limited on postharvest characteristics of this plant. Thus, there are no proposed methods to overcome their short shelflife and improve their marketability.…”