Inhibitory effect of burdock leaves on elastase and tyrosinase activity

Abstract

Abstract. Burdock (Arctium lappa L.) leaves generate a considerable amount of waste following burdock root harvest in Taiwan. To increase the use of burdock leaves, the present study investigated the optimal methods for producing burdock leaf extract (BLE) with high antioxidant polyphenolic content, including drying methods and solvent extraction concentration. In addition, the elastase and tyrosinase inhibitory activity of BLE was examined. Burdock leaves were dried by four methods: Shadow drying, oven drying, sun drying and freeze-drying. The extract solution was then subjected to total polyphenol content analysis and the method that produced BLE with the highest amount of total antioxidant components was taken forward for further analysis. The 1,1-diphenyl-2-pycrylhydrazyl scavenging, antielastase and antityrosinase activity of the BLE were measured to enable the evaluation of the antioxidant and skin aging-associated enzyme inhibitory activities of BLE. The results indicated that the total polyphenolic content following extraction with ethanol (EtOH) was highest using the freeze-drying method, followed by the oven drying, shadow drying and sun drying methods. BLE yielded a higher polyphenol content and stronger antioxidant activity as the ratio of the aqueous content of the extraction solvent used increased. BLE possesses marked tyrosinase and elastase inhibitory activities, with its antielastase activity notably stronger compared with its antityrosinase activity. These results indicate that the concentration of the extraction solvent was associated with the antioxidant and skin aging-associated enzyme inhibitory activity of BLE. The reactive oxygen species scavenging theory of skin aging may explain the tyrosinase and elastase inhibitory activity of BLE.In conclusion, the optimal method for obtaining BLE with a high antioxidant polyphenolic content was freeze-drying followed by 30-50% EtOH extraction. In addition, the antielastase and antityrosinase activities of the BLE produced may be aid in the development of skincare products with antiwrinkle and skin-evening properties. IntroductionThe skin is the largest organ of the human body and has essential functions, including protecting internal organs and regulating body temperature. Collagen and elastin, which are naturally occurring structural proteins in the dermis of the skin, maintain the plumpness, flexibility and elasticity of the skin. Skin aging presents as wrinkling, roughness, dryness, decreased elasticity and uneven pigmentation (1,2). In addition to intrinsic aging, extrinsic aging caused by exposure of the skin to solar radiation is an important concern. Reactive oxygen species (ROS) caused by ultraviolet (UV) radiation are clinically associated with specific markers of photoaging, including wrinkling, and increased elastin and collagen damage (2,3). Elastase digests elastin (4,5). Pigmentary changes due to the accumulation of melanin are another major characteristic of photoaging. Tyrosinase is a key enzyme that catalyzes melanin biosynthesis in...

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