Inhibitory Effect of Carcinogenic Aromatic Hydrocarbons on the Splenomegaly of Friend and Rauscher Virus Leukemias.

Fiscus, Alvin G.; Schloss, Gerd; Wertman, Kenneth

doi:10.3181/00379727-125-32270

Cited by 3 publications

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“…An inhibitory effect on Friend virus (FV) and Rauscher virus leukemia by the carcinogenic plycyclic aromatic hydrocarbons 7, 12-dimethylbenz [ a ] anthracene (DMlBA) and 3-methylcholanthrene was recently reported ( 1). The parameters used to show this were latent period, survival time, and spleen weight.…”

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Effect of 7,12-Dimethylbenz[ ] anthracene and Splenectomy on Virus Titer and Blood Picture in Friend Virus Leukemia

Elliott

Kiehn

Reilly

et al. 1970

Experimental Biology and Medicine

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An inhibitory effect on Friend virus (FV) and Rauscher virus leukemia by the carcinogenic plycyclic aromatic hydrocarbons 7, 12-dimethylbenz [ a ] anthracene (DMlBA) and 3-methylcholanthrene was recently reported ( 1). The parameters used to show this were latent period, survival time, and spleen weight. Interference with "indirect neoplastic effects" (development of hemorrhagic necrosis and proliferation of erythroblasts in the spleen) was considered to play a greater role than interference with a "direct neoplastic effect" (proliferation of the neoplastic reticulum cells). To investigate the mechanism of this inhibitory effect, the weekly FV titers in spleen and liver of untreated and DMBAtreated mice were compared. Also compared were the weekly liver weight and blood picture (erythrocyte volume, nucleated blood cell count, and differential blood cell count) of splenectomized, untreated and DMBAtreated, FV-infected mice with those of nonsplenectomized mice.Materials and Methods. The FV was the same as that used in the previous study (1). The titer of the virus p o l was 10490 ID50/ml. For the induction of leukemia, adult BALB/c mice, weighing approximately 25 g, received a single intraperitoneal injection of 0.2 ml of a dilution of the virus in sucrose stabilizer. For the treatment with

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“…The FV was the same as that used in the previous study (1). The titer of the virus p o l was 10490 ID50/ml.…”

mentioning

confidence: 99%

Effect of 7,12-Dimethylbenz[ ] anthracene and Splenectomy on Virus Titer and Blood Picture in Friend Virus Leukemia

Elliott

Kiehn

Reilly

et al. 1970

Experimental Biology and Medicine

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“…The depressive effect of carcinogenic polycyclic aromatic hydrocarbons (CPAH) on transformation by a DNA virus in vitro, as described in this study, may operate in a manner analogous to the previously observed phenomenon (1,2 ) of delay in malignant transformation in vivo by such carcinogens in the RNA virus-induced leukemias of Friend and Rauscher, Among the agents having an effect on viruses, interferon has attracted the greatest attention. Its influence on productive infection by many DNA and RNA viruses is well known, but its effect on transformation by some oncogenic viruses has also been reported.…”

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confidence: 76%

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Materials and Methods.

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Inhibitory Effect of Carcinogenic Aromatic Hydrocarbons on Transformation of 3T3 Cells by SV 40

Docherty

Ludovici

Schloss

1972

Experimental Biology and Medicine

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The demonstration of an inhibitory effect of 7,12-dimethylbenz[a] anthracene (DMBA) and 3-methylcholanthrene (3-MC) on Friend and Rauscher virus leukemias (1)(2)(3)(4) led to investigation of the effect of these carcinogens on other virus-induced neoplasms. Because of the difficulty in measuring objectively the inhibition of solid neoplasms in vivo, the results of the present study were based on the effect of the two carcinogens on the in vitro transformation of 3T3 cells by SV 40 virus.Materials and Methods. Cell lines and virus. The mouse fibroblast line 3T3 (5-7) was grown and maintained in Dulbecco and Vogt's modification of Eagle's basal medium, supplemented with 10% fetal calf serum, 25 units/ml of penicillin, and 25 pg/ml of streptomycin (hereafter referred to as "growth medium"). The 3T3 cells were maintained as described by Todaro and Green (6) to preserve high contact sensitivity.SV 40 virus (strain 776) was grown and titered in the African green monkey cell line BSC-1 (8) using medium 199 with Earle's balanced salt solution containing 20% fetal calf serum, 0.1% yeastolate, 50 units/ml of penicillin, and 50 pg/ml of streptomycin. The virus pool used had a titer of lo9 TCID50/ml, determined by the method of Reed and Muench (9). tests using calf SV 40 antiserum (Baltimore Biological Laboratory) , and by demonstration of the SV 40 T antigen in the nuclei of the BSC-1 cells.Chemical carcinogens. 1. Eflect on plating efficiency of 37'3 cells. Stock solutions of 4 mg of DMBA and 3-MC (Eastman Organic Chemicals) in 5 ml of dimethylsulfoxide (DMSO) were stored in amber-colored bottles at room temperature and diluted in growth medium at the time of use. To avoid toxic concentrations of the carcinogens, their effect on the plating efficiency of 3T3 cells was determined as follows. Stock 3T3 cells were dissociated with 0.25% trypsin and viable cell counts were performed in a hemocytometer using the dye exclusion technique with 0.5% trypan blue. The cell suspension was diluted so that approximately 180 viable cells were contained in 0.1 ml which was placed into each of 60 plastic tissue culture dishes. T o each of a series of 6 dishes, 0.5 ml of one of four dilutions of carcinogen (2.0, 0.2, 0.02, and 0.002 pg/O.5 ml) was added. Controls consisted of 6 dishes with untreated cells and 6 dishes with cells plus 0.5 ml of a 1 : 100 dilution of DMSO. All dishes received an additional 3.5 ml of growth medium and were incubated at 37" in a humid atmosphere containing 5% C02. The medium was replaced in all dishes on day 7. On day 14, the clones were fixed for 30 min with 10% formaldehyde in calcium-and magnesium-free phosphate-buffered saline (C'MF-PBS) and stained for 2 min with crystal violet. The plating efficiency was determined according to the formula: clones formed cells datedPlating efficiency = -x 100.2. Effect of transformation of 3T3 cells b y SV 40 virus. Three separate experiments were 969

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“…Virus. The strain of Friend virus was the same one as that used in the previous experiments (6,7). The titer of the virus pool, a 20% homogenate of leukemic spleens in sucrose stabilizer, was 104.°11D3/ml.…”

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confidence: 99%

Effect of 7,12-Dimethylbenz[a]anthracene on Phagocytosis and Antibody Formation in Friend Virus Leukemia

Elliott

Schloss

1971

Infect Immun

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BALB/c mice with Friend virus leukemia showed (i) a decreased carbon uptake by spleen and liver, observed histologically and measured by the corrected phagocytic index a, in spite of an increased total carbon clearance measured by the phagocytic index K, and (ii) a decreased serum hemolysin titer. Treatment with 7,12-dimethylbenz[a]anthracene (DMBA) increased the carbon uptake by spleen and liver and the serum hemolysin titer. This corresponds to the previously described effect of DMBA on Friend virus leukemia as expressed in the blood picture and the delay in the neoplastic transformation of the spleen causing a prolonged latent period and survival time and a decreased spleen weight. DMBA alone had no effect on carbon clearance and serum hemolysin titer in normal mice. An inhibitory effect on Friend virus (FV) leukemia by the carcinogenic polycyclic aromatic hydrocarbon 7, 12-dimethylbenz[a]anthracene (DMBA) was recently reported (6, 7). Latent

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Inhibitory Effect of Carcinogenic Aromatic Hydrocarbons on the Splenomegaly of Friend and Rauscher Virus Leukemias.

Cited by 3 publications

References 0 publications

Effect of 7,12-Dimethylbenz[ ] anthracene and Splenectomy on Virus Titer and Blood Picture in Friend Virus Leukemia

Effect of 7,12-Dimethylbenz[ ] anthracene and Splenectomy on Virus Titer and Blood Picture in Friend Virus Leukemia

Inhibitory Effect of Carcinogenic Aromatic Hydrocarbons on Transformation of 3T3 Cells by SV 40

Effect of 7,12-Dimethylbenz[a]anthracene on Phagocytosis and Antibody Formation in Friend Virus Leukemia

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