Inhibitory effect of cepharanthine on fibronectin production in growth factor-stimulated rat mesangial cells

Hayama, Misako; Inoue, Risa; Akiba, Suminori; Sato, Takashi

doi:10.1016/s0014-2999(99)00913-9

Cited by 11 publications

(7 citation statements)

References 29 publications

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“…The production of fibronectin by mesangial cells was determined as described previously ( Hayama et al ., 2000 ). The quiescent mesangial cells (35‐mm dishes) were treated with Ginkgolon‐24 for 1 h, and stimulated with oxLDL or 7‐ketocholesterol for 12 h as described in the figure legends.…”

Section: Methodssupporting

confidence: 91%

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

Akiba

Chiba

Mukaida

et al. 2004

British J Pharmacology

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1 The leaf extract of Ginkgo Biloba L. exhibits a variety of pharmacological effects through an antioxidant action. We examined the effects of the leaf extract (Ginkgolon-24) on the production of fibronectin induced by oxidized low-density lipoprotein (oxLDL) in rat mesangial cells. 2 Stimulation with oxLDL accelerated the production of fibronectin with the preceding generation of reactive oxygen species (ROS). Pretreatment with Ginkgolon-24 inhibited the oxLDL-induced fibronectin production as well as ROS generation. 3 oxLDL also elicited the activation of SP-1, nuclear factor-kB, and cAMP response elementbinding protein, which are transcription factors involved in the fibronectin production. Among these activated transcription factors, Ginkgolon-24 inhibited the activation of SP-1 only. 4 Furthermore, 7-ketocholesterol, an oxidized lipid in oxLDL particles, induced the production of fibronectin and the activation of SP-1, which were also suppressed by Ginkgolon-24. 5 These results suggest that the leaf extract of Ginkgo Biloba L. inhibits the oxLDL-induced production of fibronectin probably through inhibitory effects on ROS generation and SP-1 activation in rat mesangial cells.

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Section: Methodssupporting

confidence: 91%

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

Akiba

Chiba

Mukaida

et al. 2004

British J Pharmacology

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“…We first confirmed that PDGF significantly increased MC proliferation (Ziswiler et al, 1998;Hauser et al, 1999;Kawano et al, 2003) and fibronectin (Hayama et al, 2000) and collagen synthesis (Yamabe et al, 2000) compared to control. It is also well known that MPA inhibits MC proliferation and ECM synthesis (Ziswiler et al, 1998;Hauser et al, 1999;Dubus et al, 2002Dubus et al, , 2003.…”

Section: Discussionsupporting

confidence: 55%

Mycophenolic acid inhibits mesangial cell activation through p38 MAPK inhibition

Kim

Park

et al. 2006

Life Sciences

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“…The quiescent mesangial cells (in 35-mm dishes) were treated with various reagents and stimulated with 1 mM H 2O2 and/or 100 ng/ml PDGF-BB for 15 min as described in the figure legends. After being washed, cells were lysed in an ice-cold lysis buffer [that contained 1% Triton X-100, 150 mM NaCl, 5 mM EGTA, 50 mM NaF, 1 mM Na 3VO4, 10 g/ml leupeptin, 1 mM p-(amidinophenyl)methanesulfonyl fluoride, and 20 mM Tris ⅐ HCl at pH 7.4] and were subjected to immunoprecipitation of cPLA 2 as described previously (28). Briefly, the lysate was incubated with anti-cPLA2 antibodies overnight at 4°C and further with protein A-agarose for 2 h at 4°C.…”

Section: Methodsmentioning

confidence: 99%

ERK and p38 MAP kinase are involved in arachidonic acid release induced by H₂O₂and PDGF in mesangial cells

Hayama

Inoue

Akiba

et al. 2002

American Journal of Physiology-Renal Physiology

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-Increased prostaglandin production is implicated in the pathogenesis of glomerular disease. With this consideration, we examined the combined effects of reactive oxygen species and platelet-derived growth factor (PDGF), which might initiate glomerular dysfunction, on arachidonic acid release and cytosolic phospholipase A2 (cPLA2) activation in rat mesangial cells. H2O2-induced release of arachidonic acid was enhanced by PDGF, which by itself had little effect on the release, and the enhancement was completely inhibited by a cPLA2 inhibitor. The phosphorylation of cPLA2, extracellular signal-regulated kinase (ERK), and p38 mitogen-activated protein (MAP) kinase was upregulated by H2O2 or PDGF alone and except for ERK was enhanced further by the two in combination. The release of arachidonic acid induced by PDGF together with H2O2 was inhibited partially by an inhibitor of ERK or p38 MAP kinase and completely when the two inhibitors were combined; the inhibitory pattern was similar to that for the phosphorylation of cPLA2. These results suggest that the ERK and p38 MAP kinase pathways are involved in the increase in cPLA2 activation and arachidonic acid release induced by PDGF together with H2O2. hydrogen peroxide; p38 mitogen-activated protein kinase; extracellular signal-regulated kinase; cytosolic phospholipase A2; platelet-derived growth factor MESANGIAL CELLS SYNTHESIZE vasodilatory prostaglandins (PGs) such as PGE 2 , which act as relaxing factors and regulate glomerular hemodynamics under physiological conditions. However, an increase in renal PGE 2 production, which is observed in renal diseases such as glomerulonephritis and diabetic glomerulopathy, induces a functional disorder of the mesangium including abnormal glomerular filtration rate and is implicated in the pathogenesis of nephropathy (46,52).Previously, reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ), superoxide anion, and hydroxyl radical were recognized to cause cell injury and therefore be involved in the pathogenesis of various diseases. However, recent observations indicate that ROS cause physiological responses other than pathological responses, including protein phosphorylation, Ca 2ϩ signaling, and the activation of transcription factors by stimulating intracellular signaling systems, thus suggesting that ROS function as second messengers in the signal transduction pathway stimulated by the proinflammatory cytokines interleukin-1␤ and tumor necrosis factor-␣ (21,36,38,50).In glomerular disorders, ROS are generated by several inflammatory cells including neutrophils, monocytes, and macrophages gathered in the inflamed glomeruli, and also by the mesangial cell itself (7, 9, 10). Previous studies revealed that the intracellular generation of ROS or exposure to H 2

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Inhibitory effect of cepharanthine on fibronectin production in growth factor-stimulated rat mesangial cells

Cited by 11 publications

References 29 publications

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

Mycophenolic acid inhibits mesangial cell activation through p38 MAPK inhibition

ERK and p38 MAP kinase are involved in arachidonic acid release induced by H₂O₂and PDGF in mesangial cells

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Inhibitory effect of cepharanthine on fibronectin production in growth factor-stimulated rat mesangial cells

Cited by 11 publications

References 29 publications

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

The leaf extract of Ginkgo Biloba L. suppresses oxidized LDL‐stimulated fibronectin production through an antioxidant action in rat mesangial cells

Mycophenolic acid inhibits mesangial cell activation through p38 MAPK inhibition

ERK and p38 MAP kinase are involved in arachidonic acid release induced by H2O2and PDGF in mesangial cells

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ERK and p38 MAP kinase are involved in arachidonic acid release induced by H₂O₂and PDGF in mesangial cells