Inhibitory Effect of Coenzyme Q0 on the Growth of<i>Staphylococcus aureus</i>

Fan, Qiuxia; Yan, Chunhong; Shi, Chao; Xu, Yunfeng; Ma, Yan; Zhang, Chunyu; Peng, Xiaoli; Xia, Xiaodong

doi:10.1089/fpd.2018.2559

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…CoQ 0 has the potential activity to suppress inflammation, cancer, and metabolic diseases (Wang et al, 2017). The inhibitory effect of CoQ 0 on S. aureus development has also been reported in previous studies (Fan et al, 2019). CoQ 0 showed the ability to suppress the growth of both MRSA and VISA.…”

Section: Discussionsupporting

confidence: 83%

Coenzyme Q0 From Antrodia cinnamomea Exhibits Drug-Resistant Bacteria Eradication and Keratinocyte Inflammation Mitigation to Ameliorate Infected Atopic Dermatitis in Mouse

et al. 2019

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Atopic dermatitis (AD) is an inflammatory skin disease that is usually accompanied by Staphylococcus aureus infection due to cutaneous barrier-function damage. Benzenoid compounds from Antrodia cinnamomea are known to exhibit antibacterial and anti-inflammatory activities. This study sought to investigate the potential of benzenoids for treating bacteria-infected AD. The compounds were screened against methicillin-resistant S. aureus (MRSA). Coenzyme Q0 (CoQ0), a key ingredient in A. cinnamomea, showed the strongest MRSA growth inhibition. We further tested the inhibitory effect of CoQ0 on planktonic and biofilm MRSA. The work was also performed to explore the potential effectiveness of CoQ0 on AD using activated keratinocytes and in vivo experimental AD mice as the models. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CoQ0 against MRSA were 7.81 μg/ml. CoQ0 was found to eradicate biofilm MRSA efficiently and reduce the biofilm thickness. CoQ0 killed MRSA by inhibiting DNA polymerase and topoisomerases. A proteomic assay showed that CoQ0 also reduced the ribosomal proteins. In the anti-inflammation study, CoQ0 was found to downregulate the expression of interleukin (IL)-6, chemokine (C-C motif) ligand (CCL)5, and CCL17 in HaCaT cells. CoQ0 at 0.5 μg/ml could recover the filaggrin decreased by HaCaT activation to the normal control. We established a bacteria-infected AD-like model in mice using ovalbumin (OVA) and topically applied MRSA. Topical CoQ0 delivery lessened the MRSA presence in the AD-like lesions by >90%. The erythema, barrier function, and epidermal thickness of the AD-like wounds were improved by CoQ0 through the reduction of IL-1β, IL-4, IL-6, IL-10, interferon (IFN)-γ, and by neutrophil infiltration in the lesional skin. CoQ0 is therefore regarded as effective in mitigating AD symptoms associated with bacterial load.

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Section: Discussionsupporting

confidence: 83%

Coenzyme Q0 From Antrodia cinnamomea Exhibits Drug-Resistant Bacteria Eradication and Keratinocyte Inflammation Mitigation to Ameliorate Infected Atopic Dermatitis in Mouse

et al. 2019

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“…CLSM-based analyses showed that CoQ 0 treatment significantly reduced the integrity of S. Typhimurium cell membranes (Figure 6). Consistent with this observation, Fan et al [19] and Guo et al [17] used CLSM to show that CoQ 0 at concentrations ranging from the MIC to 4MIC caused obvious damage to the cell membranes of S. aureus and Cronobacter sakazakii. FESEM analysis also showed morphological alterations to the cell membrane following CoQ 0 treatment (Figure 7).…”

Section: Discussionmentioning

confidence: 78%

“…In the current study, CoQ 0 was shown to significantly hyperpolarize the membrane of S. Typhimurium, as shown by the negative membrane potential. We have previously shown that citral can also hyperpolarize the membrane of Cronobacter sakazakii [22], while membrane hyperpolarization was implicated as the antimicrobial mechanism of CoQ 0 against S. aureus [19]. Similarly, polyphenols extracted from olive oil can cause cell membrane depolarization in Cronobacter sakazakii and Bacillus cereus [23,35].…”

Section: Discussionmentioning

confidence: 99%

“…CoQ0 has also been shown to inhibit and kill important human pathogens such as Cronobacter sakazakii, Vibrio parahaemolyticus, Listeria monocytogenes, and Staphylococcus aureus. However, there is little information on the effects of CoQ0 on Salmonella or its ability to remove biofilms [17][18][19]. In this study, the effects of CoQ0 on S. Typhimurium were investigated to discern the mechanism of CoQ0 inhibition/killing of planktonic bacteria, and to assess its ability to eliminate S. Typhimurium biofilms.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q0 against Salmonella Typhimurium

Yang

et al. 2021

Foods

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Coenzyme Q0 (CoQ0) has anti-inflammatory and anti-tumor effects; however, the antimicrobial and antibiofilm activities of CoQ0 against Salmonella enterica serovar Typhimurium are unknown. Thus, we investigated the bacteriostatic and antibiofilm activities, along with the underlying mechanism, of CoQ0 against S. Typhimurium. The minimum inhibitory concentration (MIC) of CoQ0 against S. enterica serovars Typhimurium was 0.1–0.2 mg/mL (549–1098 µM), and CoQ0 at MIC and 2MIC decreased viable S. Typhimurium counts below detectable limits within 6 and 4 h, respectively. CoQ0 at 20MIC (4 mg/mL) reduced S. Typhimurium on raw chicken by 1.5 log CFU/cm3 within 6 h. CoQ0 effectively disrupted cell membrane integrity and induced morphological changes in the cell, resulting in hyperpolarization, decreased intracellular ATP concentrations, and cellular constituents leakage. Biofilm-associated S. Typhimurium cells were killed by CoQ0 treatment. These findings suggest that CoQ0 could be applied as a natural antibacterial substance for use against S. Typhimurium by the food industry.

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“…Cronobacter sakazakii (formerly Enterobacter sakazakii) is a motile, Gram-negative, rod-shaped opportunistic pathogen belonging to the family Enterobacteriaceae [1]. It is widely dispersed in the environment (e.g., domestic environments, manufacturing plants) and is commonly isolated from clinical specimens (e.g., cerebrospinal uid, blood, sputum), food (e.g., cheese, meat, vegetables), and animals (e.g., rats, ies) [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

Guan¹,

Shi²,

Tong³

et al. 2020

Preprint

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Objectives：Here, we investigated the inhibitory effects of coenzyme Q0 (CoQ0) on biofilm formation and the expression of virulence genes by Cronobacter sakazakii. Results：We found that the minimum inhibitory concentration of CoQ0 against C. sakazakii strains ATCC29544 and ATCC29004 was 100 μg/mL, while growth curve assays showed that sub-inhibitory concentrations (SICs) of CoQ0 for both strains were 6.4, 3.2, 1.6 and 0.8 μg/mL. Assays exploring the inhibition of specific biofilm formation showed that SICs of CoQ0 inhibited biofilm formation by C. sakazakii in a dose-dependent manner, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy analyses. CoQ0 inhibited the swimming and swarming motility of C. sakazakii and reduced its ability to adhere to and invade HT-29 cells. In addition, CoQ0 impeded the ability of C. sakazakii to survive and replicate within RAW 264.7 cells. Finally, real time polymerase chain reaction analysis confirmed that nine C. sakazakii genes associated with biofilm formation and virulence were down-regulated in response to CoQ0 treatment. Conclusion：Overall, our findings suggest that CoQ0 is a promising antibiofilm agent and provide new insights for the prevention and control of infections caused by C. sakazakii.

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Inhibitory Effect of Coenzyme Q0 on the Growth ofStaphylococcus aureus

Cited by 16 publications

References 28 publications

Coenzyme Q0 From Antrodia cinnamomea Exhibits Drug-Resistant Bacteria Eradication and Keratinocyte Inflammation Mitigation to Ameliorate Infected Atopic Dermatitis in Mouse

Coenzyme Q0 From Antrodia cinnamomea Exhibits Drug-Resistant Bacteria Eradication and Keratinocyte Inflammation Mitigation to Ameliorate Infected Atopic Dermatitis in Mouse

Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q0 against Salmonella Typhimurium

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

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