Inhibitory Effect of Delphinidin on Monocyte–Endothelial Cell Adhesion Induced by Oxidized Low-Density Lipoprotein via ROS/p38MAPK/NF-κB Pathway

Abstract

Monocyte adhesion to the vascular endothelium and their subsequent trans-endothelial migration are pivotal early events in atherogenesis. In this study, the effect of delphinidin, belonging to the group of anthocyanin, on adhesion of monocytes to endothelial cells induced by ox-LDL was investigated. The results showed that the pre-treatment with delphinidin (50, 100, or 200 μM) dose-dependently decreased the ox-LDL-induced up-regulation of the expression of ICAM-1 and P-selectin, and the enhanced adhesion and transmigration of monocytes. To determine the role of ROS/p38MAPK/NF-κB pathway, intracellular ROS level, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein, and mRNA expression were measured. The results showed that delphinidin attenuated ox-LDL-induced generation of ROS, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein and mRNA expression in endothelial cells in a dose-dependent manner. These results suggest that delphinidin attenuates ox-LDL induced expression of adhesion molecules (P-selectin and ICAM-1) and the adhesion of monocytes to endothelial cells by inhibiting ROS/p38MAPK/NF-κB pathway. These findings provide a basis for the design of potent antiatherosclerotic agents that will have therapeutic potential in the prevention of AS.

Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway

“…52 Briefly, ~30-50 μg of protein were resolved by 15% SDS-PAGE and then electroblotted onto polyvinylidene difluoride membranes for western blot analysis. Blots were probed with 1:1,000-diluted primary antibodies overnight at 4 °C, followed by horseradish peroxidase-conjugated secondary antibodies (Thermo Scientific Lab Vision, 31340 and 31455).…”

Delphinidin-3-Glucoside Protects against Oxidized Low-Density Lipoprotein-Induced Mitochondrial Dysfunction in Vascular Endothelial Cells via the Sodium-Dependent Glucose Transporter SGLT1

“…Our previous work also demonstrated that among naturally occurring ACNs, delphinidin-3-glucoside (Dp) (Fig. 1), a major ACN present in pigmented fruits and vegetables including pomegranates, berries, dark grapes, egg plant, tomatos, carrots and red onion, possesses potent activity to scavenge free radicals to inhibit endothelial oxidative injury [22]–[24]. Nevertheless, the exact molecular mechanisms of these protective effects have not yet been fully elucidated, and little is known about whether Dp possesses mitochondriotropic antioxidant activities.…”

Goyazensolide Induces Apoptosis in Cancer Cells in vitro and in vivo

“…Moreover, it has recently been reported that suppression of NF-kB activation prevents the expression of TNF-α-induced intracellular adhesion molecules, which interferes with monocyte adhesion and angiogenesis ( Addabbo et al ., 2012 , Chen et al ., 2011 , Thapa et al ., 2009 ). It has also been shown that suppression of NF-kB activation prevents the expression of intracellular adhesion molecules.…”