Inhibitory effect of (E)-2-hexenal as a potential natural fumigant on Aspergillus flavus in stored peanut seeds

Ma, Weibin; Zhao, Luling; Xie, Yanli

doi:10.1016/j.indcrop.2017.05.051

Cited by 27 publications

(17 citation statements)

References 25 publications

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“…(E)‐2‐hexenal caused remarkable membrane damage in P. cyclopium , and increased the membrane leakage of P. cyclopium spores as the concentrations of (E)‐2‐hexenal increased (Figure a). The results further confirmed the previous conclusion that (E)‐2‐hexenal showed pronounced antifungal activity against the tested fungus in a dose‐dependent manner during the incubation period (Ma, Zhao, & Xie, ; Zhang et al, ). Plasma membranes of most untreated spores (99.5%) appeared to be intact, whereas the percentages of spores exhibiting membrane leakage with 8×, and 16× MFC (E)‐2‐hexenal increased from 16.1% to 85.5%, respectively, at 2 hr.…”

Section: Resultssupporting

confidence: 90%

The effects of (E)‐2‐hexenal on morphological, physiological, and biochemical indices of postharvest disease Penicillium cyclopium spores

Zhang

Chen

et al. 2019

Journal of Food Safety

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The study investigated the effects of (E)‐2‐hexenal on morphology and function of Penicillium cyclopium spores, and these conidia were treated with (E)‐2‐hexenal at various concentrations. (E)‐2‐hexenal at 8× minimum fungicidal concentration (8× MFC, 2560 μl/L) severely damaged conidia membranes through scanning electron microscopy, which resulted in the changes in membrane permeability parameters including the release of intracellular adenosine triphosphate content, loss of 260 nm‐absorbing materials, and the decrease of extracellular pH, the increase in extracellular conductivity, and membrane leakage as determined by propidium iodide staining. (E)‐2‐hexenal (≤2 × MFC, 640 μl/L) always induced superoxide dismutase and catalase enzyme activities in P. cyclopium conidia. These results suggest that (E)‐2‐hexenal damages fungal spore membranes, and inhibit spore germination in a dose‐dependent manner, causing a series of changes in membrane permeability parameters, which serves as the inhibitory mechanism of (E)‐2‐hexenal against P. cyclopium. Practical applications (E)‐2‐hexenal treatment significantly resulted in serious detrimental damages in the morphology and function in P. cyclopium spores. The work presented (E)‐2‐hexenal could control fungal pathogens through disruption of cell membrane integrity and membrane permeability. The results revealed that (E)‐2‐hexenal can be used as a new anti‐fungal agent against P. cyclopium.

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Section: Resultssupporting

confidence: 90%

The effects of (E)‐2‐hexenal on morphological, physiological, and biochemical indices of postharvest disease Penicillium cyclopium spores

Zhang

Chen

et al. 2019

Journal of Food Safety

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“…The biocidal properties of POs could be linked to various lethal mechanisms such as membrane pore formation, membrane destabilisation, protein or nucleic acid denaturation, or oxidative bursts. In vitro assays notably showed that 2(E)-hexenal exhibits the highest efficacy among POs (Table 1), with detergent-like action that severely damages membranes and cell walls [70]. Such behaviour is typically observed with reactive electrophiles species (RES).…”

Section: The Road To the Next Levelmentioning

confidence: 99%

Plant–Pathogen Interactions: Underestimated Roles of Phyto-oxylipins

Deboever

Deleu

Mongrand

et al. 2020

Trends in Plant Science

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Almost 30 years ago, the term 'oxylipin' (see Glossary) appeared in the literature and since then, publications on the topic have increased steadily. Oxylipins are found in almost all organisms and are present in free forms, esterified to phospholipids or galactolipids, or combined with other compounds (e.g., methyl groups, isoleucine) [1-10]. The precursors of oxylipin synthesis vary among organisms, as do the enzymes that will oxidise them. Because aerobic biological systems are continuously subject to autooxidation, oxylipins (e.g., phytoprostanes) are also produced through nonenzymatic routes in the presence of singlet oxygen or reactive oxygen species (ROS) [3,11-15]. Both pathways have been extensively reviewed. Figure 1 summarises the enzymatic production pathways of oxylipins (free forms) in mammals, fungi, and flowering plants (for detailed information see [8,13-21]).

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“…Hence, the control of A. flavus and AFs in storage is more difficult than other pathogens without conidia. Recently, Ma et al (2017) report that the biocontrol activity of volatile phase on A. flavus is more effective than contact phase. Hence, screening antagonistic volatiles in control post-harvest A. flavus and AFs is urgent nowadays.…”

Section: Discussionmentioning

confidence: 99%

“…Compared to these contact phase, volatile phase can easily and uniformly distribute in the whole space of storage, and generated lower residual after volatilization. Recently, some evidences proved that the inhibitory effect of volatile phase on A. flavus in storage was consistently more effective than contact phase (Hu et al, 2017; Ma et al, 2017). To date, seldom microbe volatile was identified and used to control A. flavus and AFs during storage.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Volatiles Emitted From Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in Storage

Gong

Kong

et al. 2019

Front. Microbiol.

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Controlling aflatoxigenic Aspergillus flavus and aflatoxins (AFs) in grains and food during storage is a great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of A. flavus in un-contacted face-to-face dual culture testing. Gas chromatography tandem mass spectrometry revealed that dimethyl disulfide (DMDS) and methyl isovalerate (MI) were two abundant compounds in the volatile profiles of N1-4. DMDS was found to have the highest relative abundance (69.90%, to the total peak area) in N1-4, which prevented the conidia germination and mycelial growth of A. flavus at 50 and 100 μL/L, respectively. The effective concentration for MI against A. flavus is 200 μL/L. Additionally, Real-time quantitative PCR analysis proved that the expression of 12 important genes in aflatoxin biosynthesis pathway was reduced by these volatiles, and eight genes were down regulated by 4.39 to 32.25-folds compared to control treatment with significant differences. And the A. flavus infection and AFs contamination in groundnut, maize, rice and soybean of high water activity were completely inhibited by volatiles from N1-4 in storage. Scanning electron microscope further proved that A. flavus conidia inoculated on peanuts surface were severely damaged by volatiles from N1-4. Furthermore, strain N1-4 showed broad and antifungal activity to other six important plant pathogens including Fusarium graminearum, F. equiseti, Alternaria alternata, Botrytis cinerea, Aspergillus niger , and Colletotrichum graminicola. Thus, A. faecalis N1-4 and volatile DMDS and MI may have potential to be used as biocontrol agents to control A. flavus and AFs during storage.

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Inhibitory effect of (E)-2-hexenal as a potential natural fumigant on Aspergillus flavus in stored peanut seeds

Cited by 27 publications

References 25 publications

The effects of (E)‐2‐hexenal on morphological, physiological, and biochemical indices of postharvest disease Penicillium cyclopium spores

The effects of (E)‐2‐hexenal on morphological, physiological, and biochemical indices of postharvest disease Penicillium cyclopium spores

Plant–Pathogen Interactions: Underestimated Roles of Phyto-oxylipins

Inhibitory Effect of Volatiles Emitted From Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in Storage

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