Inhibitory Effect of Eicosapentaenoic Acid on the Migration of the Esophageal Squamous Cell Carcinoma Cell Line TE-1

Abstract

Background/Aim: Eicosapentaenoic acid (EPA) inhibits NF-ĸB activation and IL-6 production in TE-1 esophageal cancer cells. NF-ĸB is related to cancer cell migration. The aim of this study is to evaluate whether EPA has a metastasis suppressing effect. Herein, we investigated EPA-treated TE-1 cell migration using TAXIScan. Materials and Methods: EZ-TAXIScan ® was used to verify whether EPA inhibits cancer cell chemotaxis. Results: Using 50% fetal bovine serum (chemoattractant) without EPA (positive control), average velocity was 0.306±0.084 μm/min compared to 0.162±0.067 μm/min without chemoattraction (negative control). Directionalities of positive and negative controls were 1.039±0.152 and 0.488±0.251 radians, respectively, indicating a significant increase in migration of the positive control compared to that of the negative control. Average velocities were 0.306±0.084 (no EPA), 0.288±0.078 (100 μM EPA), and 0.240±0.054 200 μM (EPA) μm/min, indicating that EPA reduced velocity dose-dependently. Average directionalities were 1. 039±0.152 (no EPA), 0.967±0.164 (100 μM EPA), and 0.901±0.146 (200 μM EPA) radians, indicating that EPA also inhibited directionality dose-dependently. Conclusion: EPA suppresses directional migration of TE-1 cells.

The Influences of Omega-3 Polyunsaturated Fatty Acids on the Development of Skin Cancers

“…Furthermore, EPA may suppress the migration of SCC cells and reduce migration velocity and directionality in a dose-dependent manner [81].…”

“…Epidemiological analyses have shown the beneficial potency of omega-3 PUFAs in melanoma [27], basal cell carcinoma [56], and diffuse large B cell lymphoma [69]. In addition, omega-3 PUFAs suppress tumor growth in melanoma [32,37,41,42,44,45] and squamous cell carcinoma [77], inhibiting migration and invasion in melanoma [33,34,37] and squamous cell carcinoma [81]. Omega-3 PUFAs also enhance the sensitivity to chemotherapy in melanoma [28][29][30][31][46][47][48] and radiosensitivity in squamous cell carcinoma [78].…”

“…Suppress cell proliferation by EPA [77] Suppress tumor growth by the combination of Omega-3 PUFAs with ionizing radiation [78]. Suppress migration by EPA [81].…”