Inhibitory effect of Ephedra herba on human norovirus infection in human intestinal organoids

Hayashi, Tsuyoshi; Murakami, Kosuke; Ando, Hirokazu; Ueno, Sayuri; Kobayashi, Sakura; Muramatsu, Masamichi; Tanikawa, Takashi; Kitamura, Masashi

doi:10.1016/j.bbrc.2023.05.127

Cited by 10 publications

(3 citation statements)

References 22 publications

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“…This comparison, not previously undertaken in other studies that used OGM or ODM media (28,(39)(40)(41)(42)(43), provides guidance for laboratories using commercial media for HuNoV studies. Further, an evaluation of newly established lines, derived from intestinal segments of different donors, highlights strain-, donor-and segment-specific differences within the GII genogroup of HuNoVs.…”

Section: Discussionmentioning

confidence: 94%

“…This development overcame a five-decade barrier in laboratory models for HuNoVs, opening new avenues for investigating HuNoV biology. Since the establishment of the HIE system, significant advancements have been made in unraveling various aspects of HuNoV biology, including strain-specific differences in replication, methods of virus inactivation, innate immune responses, antiviral susceptibility, and characterization of neutralizing antibodies (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Here, we report further advancements in the HIE culture system by testing HuNoV infection in multiple unique HIE lines and expanding the profile of cultivatable HuNoV strains.…”

Section: Introductionmentioning

confidence: 99%

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Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

Ettayebi,

Kaur,

Patil

et al. 2024

Preprint

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Human noroviruses (HuNoVs) are a significant cause of both epidemic and sporadic acute gastroenteritis worldwide. The lack of a reproducible culture system for HuNoVs was a major obstacle in studying virus replication and pathogenesis for almost a half- century. This barrier was overcome with our successful cultivation of multiple HuNoV strains in human intestinal enteroids (HIEs), which has significantly advanced HuNoV research. We previously optimized culture media conditions and generated genetically-modified HIE cultures to enhance HuNoV replication in HIEs. Building upon these achievements, we now present additional advancements to this culture system, which involve testing different media, unique HIE lines, and additional virus strains. HuNoV infectivity was evaluated and compared in new HIE models, including HIEs generated from different intestinal segments of individual adult organ donors, HIEs made from human embryonic stem cell-derived human intestinal organoids that were transplanted into mice (H9tHIEs), genetically-engineered (J4FUT2 knock-in [KI], J2STAT1 knock-out [KO]) HIEs, as well as HIEs derived from a patient with common variable immunodeficiency (CVID) and from infants. Our findings reveal that small intestinal HIEs, but not colonoids, from adults, H9tHIEs, HIEs from a CVID patient, and HIEs from infants support HuNoV replication with segment and strain-specific differences in viral infection. J4FUT2-KI HIEs exhibit the highest susceptibility to HuNoV infection, allowing the cultivation of a broader range of GI and GII HuNoV strains than previously reported. Overall, these results contribute to a deeper understanding of HuNoVs and highlight the transformative potential of HIE cultures in HuNoV research.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

Ettayebi,

Kaur,

Patil

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…Human norovirus (HuNoV) also belongs to the family Caliciviridae , but it cannot sufficiently replicate in a cell culture system. Although relevant organoid models have been developed for drug screening, developing relevant antiviral drugs remains difficult due to insufficient replication of HuNoV in a cell culture system [45, 46]. FCV has been used as a surrogate model for the screening of effective drugs for HuNoVs in a previous study [10].…”

Section: Discussionmentioning

confidence: 99%

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Yan,

Yang,

Jiao

et al. 2024

Journal of General Virology

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Feline calicivirus (FCV) is considered one of the major pathogens of cats worldwide and causes upper respiratory tract disease in all cats. In some cats, infection is by a highly virulent strain of FCV (vs.-FCV), which can cause severe and fatal systemic disease symptoms. At present, few antiviral drugs are approved for clinical treatment against FCV. Therefore, there is an imminent need for effective FCV antiviral agents. Here, we used observed a cytopathic effect (CPE) assay to screen 1746 traditional Chinese medicine monomer compounds and found one that can effectively inhibit FCV replication, namely, handelin, with an effective concentration (EC50) value of approximately 2.5 µM. Further study showed that handelin inhibits FCV replication via interference with heat shock protein 70 (HSP70), which is a crucial host factor and plays a positive role in regulating viral replication. Moreover, handelin and HSP70 inhibitors have broad-spectrum antiviral activity. These findings indicate that handelin is a potential candidate for the treatment of FCV infection and that HSP70 may be an important drug target.

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Antiviral Activity of Natural Compounds for Food Safety

Falcó,

Randazzo,

Sánchez

2024

Food Environ Virol

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Gastroenteritis and hepatitis are the most common illnesses resulting from the consumption of food contaminated with human enteric viruses. Several natural compounds have demonstrated antiviral activity against human enteric viruses, such as human norovirus and hepatitis A virus, while little information is available for hepatitis E virus. Many in-vitro studies have evaluated the efficacy of different natural compounds against human enteric viruses or their surrogates. However, only few studies have investigated their antiviral activity in food applications. Among them, green tea extract, grape seed extract and carrageenans have been extensively investigated as antiviral natural compounds to improve food safety. Indeed, these extracts have been studied as sanitizers on food-contact surfaces, in produce washing solutions, as active fractions in antiviral food-packaging materials, and in edible coatings. The most innovative applications of these antiviral natural extracts include the development of coatings to extend the shelf life of berries or their combination with established food technologies for improved processes. This review summarizes existing knowledge in the underexplored field of natural compounds for enhancing the safety of viral-contaminated foods and underscores the research needs to be covered in the near future.

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Inhibitory effect of Ephedra herba on human norovirus infection in human intestinal organoids

Cited by 10 publications

References 22 publications

Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Antiviral Activity of Natural Compounds for Food Safety

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