Inhibitory effect of esculetin on migration, invasion and matrix metalloproteinase-9 expression in TNF-α-induced vascular smooth muscle cells

Lee, Se-Jung; Lee, Ung‐Soo; Kim, Wun-Jae; Moon, Sung‐Kwon

doi:10.3892/mmr.2011.420

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…The cell cycle profiles in cells expressing HPV E7 under hypoxia were not clear. Glioma cells expressing HPV-16 E7 showed a concomitant decrease in G1 and S phases subject to hypoxia 34 . Here we demonstrated that HPV-16 E7 abrogates G1 checkpoint under hypoxia and rendered cells enter into S-phase.…”

Section: Discussionmentioning

confidence: 99%

“…In cervical cancer, HPV E7 increases HIF-1α mediated transcription by inhibiting the binding of histone deacetylases 31 , leading to HIF-1α accumulation and VEGF expression, which may contribute to enhanced angiogenesis 32, 33 . Glioma cells expressing HPV-16 E7 showed a G2/M arrest with concomitant decrease in G1 and S phases subject to hypoxia 34 . The cell cycle profiles in other types of cells expressing HPV E7 under hypoxia remain to be determined.…”

Section: Introductionmentioning

confidence: 99%

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Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Zhang

Qiao

Fan

et al. 2017

Sci Rep

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The human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for their potential role in this process. To further explore the mechanism by which E7 bypasses hypoxia-induced G1 arrest, we applied a proteomic approach and used mass spectrometry to search for proteins that are differentially expressed in E7 expressing cells under hypoxia. Among differentially expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic activities when overexpressed. We have recently demonstrated that Cdc6 was required for E7-induced re-replication. Significantly, here we showed that Cdc6 played a role in E7-mediated G1 checkpoint abrogation under hypoxic condition, and the function could possibly be independent from its role in DNA replication initiation. This study uncovered a new function of Cdc6 in regulating cell cycle progression and has important implications in HPV-associated cancers.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Zhang

Qiao

Fan

et al. 2017

Sci Rep

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“…As shown in Table I, only two 4 . Potentials are given with respect to a cadmium-saturated mercury amalgam reference electrode in contact with DMF saturated with both cadmium chloride and sodium chloride; this electrode has a potential of -0.76 V versus SCE at 25 • C. For the cyclic voltammogram recorded with glassy carbon, the potential was scanned from 0 to -2.0 to 0 V; for the cyclic voltammogram recorded with silver, the potential was scanned from 0 to -1.8 to 0 V. cathodic peaks are observed when silver is employed as an electrode for compounds 2a-5a; we believe that these two peaks correspond to the first two stages for the reduction of 1a.…”

Section: Resultsmentioning

confidence: 96%

“…1 Coumarin and its derivatives exhibit biological activity and could possibly be used as anti-coagulant, 2 antitumor, 3 anti-inflammatory, 4 antibiotic, 5 anti-HIV-1, 5 anti-diabetic, 6 and anti-depressant drugs. 6 Two of the most well-known coumarin derivatives are (RS)-4-hydroxy-3-(3-oxo-1-phenylbutyl)-2H-chromen-2-one (Warfarin) and its sodium salt (Coumadin), both of which are prescribed as anti-coagulants.…”

mentioning

confidence: 99%

Reduction of Substituted Phenyl 2-Chloroacetates at Silver Cathodes: Electrosynthesis of Coumarins

Pasciak

Peters

2014

J. Electrochem. Soc.

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To explore the electrosynthesis of coumarins, cyclic voltammetry and controlled-potential (bulk) electrolysis have been employed to investigate the reduction of the carbon-chlorine bond of five substituted phenyl 2-chloroacetates at silver cathodes in dimethylformamide (DMF) containing 0.10 M tetra-n-butylammonium tetrafluoroborate (TBABF 4 ) as supporting electrolyte; the five substrates are 2-formylphenyl 2-chloroacetate (1a), 2-acetylphenyl 2-chloroacetate (2a), methyl 2-(2-chloroacetoxy)benzoate (3a), 2-formyl-5-methoxyphenyl 2-chloroacetate (4a), and 2-formyl-3,5-dimethoxyphenyl 2-chloroacetate (5a). We have examined (a) the effects of substituents on the benzene ring of the substrate as well as the nature of the aryl carbonyl moiety on the formation of the coumarin product and (b) the effect of solvent-namely, DMF, acetonitrile (CH 3 CN), benzonitrile (PhCN), and propylene carbonate (PC)-and substrate concentration on the yield of the coumarin. It was found that the most unsubstituted substrate (1a) afforded the highest yield (41%) of the desired coumarin in a DMF-TBABF 4 medium. A mechanistic scheme is proposed to account for the formation of the coumarin. Furthermore, the only other products seen in these reductions are 2-substituted phenols, which are precursors for synthesis of the various substrates.Coumarins have become important in many fields such as biology, medicine, and organic chemistry; for example, coumarins have been employed as polymers for industry. 1 Coumarin and its derivatives exhibit biological activity and could possibly be used as anti-coagulant, 2 antitumor, 3 anti-inflammatory, 4 antibiotic, 5 anti-HIV-1, 5 anti-diabetic, 6 and anti-depressant drugs. 6 Two of the most well-known coumarin derivatives are (RS)-4-hydroxy-3-(3-oxo-1-phenylbutyl)-2H-chromen-2-one (Warfarin) and its sodium salt (Coumadin), both of which are prescribed as anti-coagulants. Additionally, optical properties of coumarins allow them to be employed in applications such as laser devices, light-emitting diodes, and fluorescent markers in biomedical imaging. 7 There are many classic processes for the synthesis of coumarins, 8-12 including the use of transition-metal catalysts; 13-19 however, among all of these methods, the most commonly employed is the Pechmann reaction. 8 Classic approaches allow a large range of derivatives to be prepared, but these methods usually require harsh conditions, such as addition of strong acids, and can afford mixtures of products that are difficult to separate.Despite the many applications of coumarins mentioned above, very few publications can be found that deal with the electrosynthesis of these compounds. In comparison with traditional methods, electrosynthesis can provide many advantages, such as short reaction times, mild reagents, low energy cost, and easily isolated products; and, in most cases, the reaction can be conducted at room temperature. Batanero and co-workers 20-22 studied the electrochemical reduction of various substituted phenyl 2,2,2-trichloroacetates at a mercur...

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“…Commonly used TNFα concentrations with physiological effects are between 10–100 ng/ml [29], [30], [31]. We used concentration of 50 ng/ml for our studies.…”

Section: Discussionmentioning

confidence: 99%

Dual Regulation of Myocardin Expression by Tumor Necrosis Factor-α in Vascular Smooth Muscle Cells

Singh

Zheng

2014

PLoS ONE

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De-differentiation of vascular smooth muscle cells (VSMCs) plays a critical role in the development of atherosclerosis, a chronic inflammatory disease involving various cytokines such as tumor necrosis factor-α (TNFα). Myocardin is a co-factor of serum response factor (SRF) and is considered to be the master regulator of VSMC differentiation. It binds to SRF and regulates the expression of contractile proteins in VSMCs. Myocardin is also known to inhibit VSMC proliferation by inhibiting the NF-κB pathway, whereas TNFα is known to activate the NF-κB pathway in VSMCs. NF-κB activation has also been shown to inhibit myocardin expression and smooth muscle contractile marker genes. However, it is not definitively known whether TNFα regulates the expression and activity of myocardin in VSMCs. The current study aimed to investigate the role of TNFα in regulating myocardin and VSMC function. Our studies showed that TNFα down-regulated myocardin expression and activity in cultured VSMCs by activating the NF-κB pathway, resulting in decreased VSMC contractility and increased VSMC proliferation. Surprisingly, we also found that TNFα prevented myocardin mRNA degradation, and resulted in a further significant increase in myocardin expression and activity in differentiated VSMCs. Both the NF-κB and p44/42 MAPK pathways were involved in TNFα regulation of myocardin, which further increased the contractility of VSMCs. These differential effects of TNFα on myocardin seemingly depended on whether VSMCs were in a differentiated or de-differentiated state. Taken together, our results demonstrate that TNFα differentially regulates myocardin expression and activity, which may play a key role in regulating VSMC functions.

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Inhibitory effect of esculetin on migration, invasion and matrix metalloproteinase-9 expression in TNF-α-induced vascular smooth muscle cells

Cited by 13 publications

References 16 publications

Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Reduction of Substituted Phenyl 2-Chloroacetates at Silver Cathodes: Electrosynthesis of Coumarins

Dual Regulation of Myocardin Expression by Tumor Necrosis Factor-α in Vascular Smooth Muscle Cells

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