Inhibitory Effect of Forskolin on Myosin Phosphorylation-Dependent and Independent Contractions in Bovine Tracheal Smooth Muscle.

Tajimi, Masaomi; Hori, Masaru; Mitsui, Minori; Ozaki, Hiroshi; Karaki, Hideaki

doi:10.1540/jsmr.31.129

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…In bovine trachea, it has been reported that forskolin increased cAMP content and inhibited high K + -induced contraction by decreasing Ca 2+ sensitivity of the contractile protein (34). In the present experiments, forskolin also increased cAMP content and inhibited Ca…”

Section: Elucidatedsupporting

confidence: 70%

Inhibitory Mechanism of Monensin on High K+-Induced Contraction in Guniea-Pig Urinary Bladder

et al. 2006

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Abstract. In this study, we examined the inhibitory mechanism of monensin on high K + -induced contraction in guinea-pig urinary bladder. The relaxant effect of monensin (0.001 -10 µM) was more potent than those of NaCN (100 µM -1 mM) and forskolin (3 -10 µM).] i level. Monensin and NaCN remarkably decreased creatine phosphate and ATP contents. Monensin and NaCN inhibited high K + -induced increases in flavoprotein fluorescence, which is involved in mitochondrial respiration. Forskolin increased cAMP content but monensin did not. Monensin increased Na + content at 10 µM but not at 0.1 µM that induced maximum relaxation. In the α-toxin-permeabilized muscle, forskolin significantly inhibited the Ca 2+ -induced contraction, but monensin did not affect it. These results suggest that the relaxation mechanism of monensin in smooth muscle of urinary bladder may be an inhibition of oxidative metabolism.

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Section: Elucidatedsupporting

confidence: 70%

Inhibitory Mechanism of Monensin on High K+-Induced Contraction in Guniea-Pig Urinary Bladder

et al. 2006

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“…Forskolin (as dry powder capsules) was found to produce bronchodilatation in 16 patients with asthma [82]. A number of studies, using animal models, demonstrated several mechanisms of forskolin to relax pulmonary smooth muscle such as the cAMP-dependent protein kinase (PKA) mediated inhibition of endothelin-1 stimulated generation of inositol phosphates and Ca 2+ mobilization [83], the inhibition of the binding of inositol 1,4,5-triphosphate (IP 3 ) to its Ca 2+ -mobilizing intracellular receptor [84], the protein kinase C isoenzymes mediated activation of large-conductance, calcium-and voltage-activated potassium channels and the resulting pulmonary vasodilatation [85], the decrease in the Ca 2+ oscillation frequency of airway smooth muscle cells by reducing internal Ca 2+ release through IP 3 receptors [86], as well as the inhibition of myosin light chain (MLC) phosphorylation by reducing Ca 2+ influx, Ca 2+ release, by changing the MLC kinase/phosphatase balance and the inhibition of the MLC phosphorylation-independent regulatory mechanism [87]. It has also been reported that the relaxant effects of forskolin in cultured rat tracheal smooth muscle might be mediated, at least in part, by facilitating the sequestration of Ca 2+ into intracellular stores by a mechanism involving guanosine 3′,5′-cyclic monophosphate-dependent protein kinase [88].…”

Section: Forskolinmentioning

confidence: 99%

Plectranthus barbatus: A Review of Phytochemistry, Ethnobotanical Uses and Pharmacology – Part 2

Alasbahi

Melzig²

2010

Planta Med

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Pharmacology ! Plectranthus barbatusStudies on several extracts with various polarities obtained from different parts of P. barbatus revealed a number of pharmacological properties that may justify most of the traditional uses of P. barbatus, for example, organic extracts were reported to possess anti-inflammatory, antimicrobial [1, 2], antioxidant [3], cytotoxic [4], hypotensive [5,6], spasmolytic [7], hepatoprotective [8] and antifeedant [9] activities as well as activity against Ehrlichʼs ascites tumor in mice [10]. An alcoholic extract of the P. barbatus roots was shown to exhibit marked inhibitory action against Escherichia coli toxin-induced secretory response in rabbits and guinea pig ileal loops [11]. It has been shown that a hydroalcoholic extract of P. barbatus (880 mg/kg/day) exerted a variety of toxic effects on the different periods of pregnancy in rats; for example, in the period before embryo implantation, it caused a delay in fetal development and anti-implantation and, after embryo implantation, a delay in the development associated with maternal toxicity [12]. The water extract of P. barbatus leaves was found to exert hypoglycemic, hypotensive and antispasmodic activities [13]. It has been demonstrated that the water extract (WE) of the stems and leaves, at a dose of 1 g/kg p. o., shortened the sleeping time induced by pentobarbital by 37 %, and at a dose of 2 g/kg p. o. enhanced the intestinal transit of charcoal by 30% in mice. The WE (2 g/kg/intraduodenal) also reduced the gastric secretions (3.9 ± 1.0 to 0.5 ± 0.2 mL/4 h), and total acid secretion (34.4 ± 11.0 to 2.7 ± 0.5 mEq/L) and raised gastric pH (2.2 ± 0.3 to 6.5 ± 0.8) in rats. The treatment with WE (2 g/ kg p. o.) was also found to protect against lesions induced by ethanol or cold-restraint stress, in pylorus-ligated rats [14]. Similar results were obtained by Schultz et al. [15] who reported that the WE of the leaves (0.5-0.1 g/kg) injected into the duodenal lumen decreased the volume (62 and 76 %) and total acidity (23 and 50%) of gastric Abstract ! Plectranthus barbatus Andr. is one of the most important species of the genus Plectranthus L′ Herit. (Lamiaceae), with a wide variety of traditional medicinal uses in Hindu and Ayurvedic traditional medicine as well as in the folk medicine of Brazil, tropical Africa and China. The plant has therefore been an attractive target for intensive chemical and pharmacological studies up to now. This review presents data about the phytochemistry, ethnobotanical uses and pharmacology of Plectranthus barbatus as well as the pharmacology of its constituents. In addition to essential oil, abietane diterpenoids and 8,13-epoxy-labd-14-en-11-one diterpenoids are the main constituents found in Plectranthus barbatus. The major ethnobotanical uses are for intestinal disturbance and liver fatigue, respiratory disorders, heart diseases and certain nervous system disorders. Forskolin as one of the major constituents with its unique adenylyl cyclase activation that underlies the wide range of pharmacologic...

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“…] i level was measured simultaneously with a muscle contraction as reported previously (28). Muscle strips were incubated with PSS containing 5 µM fura2 / AM for overnight at 4°C.…”

Section: +mentioning

confidence: 99%

Inhibitory Mechanism of Papaverine on Carbachol-Induced Contraction in Bovine Trachea

et al. 2005

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Abstract. We have demonstrated that the relaxing mechanism of papaverine in phasic muscles such as ileum, urinary bladder, and uterus is different from tonic muscles such as aorta. In this study, we examined the inhibitory mechanism of papaverine on carbachol ( ] i level induced by papaverine or dibutyryl-cAMP. These results suggested that the relaxing mechanism of papaverine in the bovine trachea is mainly due to increases of cAMP content by inhibiting phosphodiesterase and the mechanism is partially involved in the activation of BK channel by cAMP.

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Inhibitory Effect of Forskolin on Myosin Phosphorylation-Dependent and Independent Contractions in Bovine Tracheal Smooth Muscle.

Abstract: In bovine tracheal smooth

Cited by 8 publications

References 40 publications

Inhibitory Mechanism of Monensin on High K+-Induced Contraction in Guniea-Pig Urinary Bladder

Inhibitory Mechanism of Monensin on High K+-Induced Contraction in Guniea-Pig Urinary Bladder

Plectranthus barbatus: A Review of Phytochemistry, Ethnobotanical Uses and Pharmacology – Part 2

Inhibitory Mechanism of Papaverine on Carbachol-Induced Contraction in Bovine Trachea

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