Inhibitory Effect of Hexane Fraction from Myagropsis myagroides on Pancreatic ��-Amylase In Vitro

Pak, Won-Min; Kim, Koth-Bong-Woo Ri; Kim, Minji; Cho, Ji-Young; Ahn, Dong-Hyun

doi:10.4014/jmb.1409.09012

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…Debasis et al (2013) had observed the highest α-amylase and α-glucosidase inhibitory activity in the n-hexane fraction of sepal of Salmalia malabarica [31]. The n-hexane fraction of Myagropsis myagroides showed significant amylase inhibition [32]. Saleh et al (2013) observed significant α-glucosidase and α-amylase inhibitory in the n-hexane fractions of ten plant extracts traditionally used in Iran for diabetes [33,34].…”

Section: α-Amylase and α-Glucosidase Inhibition Studiesmentioning

confidence: 99%

Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia

et al. 2019

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Nuxia oppositifolia is traditionally used in diabetes treatment in many Arabian countries; however, scientific evidence is lacking. Hence, the present study explored the antidiabetic and antioxidant activities of the plant extracts and their purified compounds. The methanolic crude extract of N. oppositifolia was partitioned using a two-solvent system. The n-hexane fraction was purified by silica gel column chromatography to yield several compounds including katononic acid and 3-oxolupenal. Antidiabetic activities were assessed by α-amylase and α-glucosidase enzyme inhibition. Antioxidant capacities were examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging assays. Further, the interaction between enzymes (α-amylase and α-glucosidase) and ligands (3-oxolupenal and katononic acid) was followed by fluorescence quenching and molecular docking studies. 3-oxolupenal and katononic acid showed IC50 values of 46.2 μg/mL (101.6 µM) and 52.4 μg/mL (119.3 µM), respectively against the amylase inhibition. 3-oxolupenal (62.3 µg/mL or 141.9 μM) exhibited more potent inhibition against α-glucosidases compared to katononic acid (88.6 µg/mL or 194.8 μM). In terms of antioxidant activity, the relatively polar crude extract and n-butanol fraction showed the greatest DPPH and ABTS scavenging activity. However, the antioxidant activities of the purified compounds were in the low to moderate range. Molecular docking studies confirmed that 3-oxolupenal and katononic acid interacted strongly with the active site residues of both α-amylase and α-glucosidase. Fluorescence quenching results also suggest that 3-oxolupenal and katononic acid have a good affinity towards both α-amylase and α-glucosidase enzymes. This study provides preliminary data for the plant’s use in the treatment of type 2 diabetes mellitus.

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Section: α-Amylase and α-Glucosidase Inhibition Studiesmentioning

confidence: 99%

Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia

et al. 2019

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“…For example SCA-C contains a 9′-hydroxyl group with R-configuration in the side-chain, while SCA-F has a methoxy group at C-9′ and a hydroxyl group with Rconfiguration at C-10′ (extensively reviewed in Birringer et al, 2018). SCAs have been reported to exhibit various biological activities, including anti-oxidative (Lim et al, 2019) (SCA-G), anti-osteoclastogenic (Yoon et al, 2012b;Yoon et al, 2013) (SCA-G), anti-inflammatory (Yoon et al, 2012a;Lee et al, 2013;Heo et al, 2014) (SCA-G and SCA-D), as well as antidiabetic (Pak et al, 2015) (SCA-I) ones. To the best of our knowledge, metabolism of sargachromanols in humans or animals has not been investigated.…”

Section: Sargachromanolsmentioning

confidence: 99%

Diversity of Chromanol and Chromenol Structures and Functions: An Emerging Class of Anti-Inflammatory and Anti-Carcinogenic Agents

et al. 2020

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Natural chromanols and chromenols comprise a family of molecules with enormous structural diversity and biological activities of pharmacological interest. A recently published systematic review described more than 230 structures that are derived from a chromanol ortpd chromenol core. For many of these compounds structure-activity relationships have been described with mostly anti-inflammatory as well as anticarcinogenic activities. To extend the knowledge on the biological activity and the therapeutic potential of these promising class of natural compounds, we here present a report on selected chromanols and chromenols based on the availability of data on signaling pathways involved in inflammation, apoptosis, cell proliferation, and carcinogenesis. The chromanol and chromenol derivatives seem to bind or to interfere with several molecular targets and pathways, including 5-lipoxygenase, nuclear receptors, and the nuclear-factor "kappa-light-chain-enhancer" of activated B-cells (NFkB) pathway. Interestingly, available data suggest that the chromanols and chromenols are promiscuitively acting molecules that inhibit enzyme activities, bind to cellular receptors, and modulate mitochondrial function as well as gene expression. It is also noteworthy that the molecular modes of actions by which the chromanols and chromenols exert their effects strongly depend on the concentrations of the compounds. Thereby, low-and highaffinity molecular targets can be classified. This review summarizes the available knowledge on the biological activity of selected chromanols and chromenols which may represent interesting lead structures for the development of therapeutic anti-inflammatory and chemopreventive approaches.

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“…Sagachromanols (1~16) were isolated from the brown alga Sargassum siliquastrum as novel bioactive substances [12] . In a previous study [13] , sargachromanol I was isolated from M. myagroides, which inhibited amylase activity. In this study, in vitro anticollagenase and free radical scavenging activities of M. myagroides extracts were investigated, particularly the hexane fraction, and the isolated active compound sargachromanol I.…”

mentioning

confidence: 98%

Free Radical Scavenging and Anticollagenase Properties of Sargachromanol I from Myagropsis myagroides

Kim¹,

Kim²,

Jeong³

et al. 2019

pharmaceutical-sciences

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Kim et al.: Anticollagenase and Free Radical Scavenging Properties of Sargachromanol I The present study investigated the free radical scavenging activity and antiwrinkle properties of methanol extract, hexane, chloroform, ethyl acetate, and butanol partition fractions of Myagropsis myagroides. The methanol extract showed higher free radical scavenging (65.46 % at 0.5 mg/ml) and collagenase inhibitory activity (29.64 % at 1000 μg/ml) than the water extract (16.68 % at 0.5 mg/ml and <5 % at 1000 μg/ml). The hexane fraction exhibited strong free radical scavenging activity (96.36 % at 0.5 mg/ml) and collagenase inhibitory activity (IC 50 of 712.21 μg/ml). The hexane fraction was subjected to silica gel column chromatography, Sephadex LH-20 column chromatography, octadecyl silica Sepak cartridge, and preparative high performance liquid chromatography. Sargachromaol I was isolated, which showed strong free radical scavenging activity (95.82 % at 0.1 mg/ml) and collagenase inhibitory activity (IC 50 value of 54.01 μg/ml). The potential value in preventing skin aging could be confirmed through effects of extract from Myagropsis myagroides on free radical scavenging activity and collagenase inhibitory properties.

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Inhibitory Effect of Hexane Fraction from Myagropsis myagroides on Pancreatic ��-Amylase In Vitro

Cited by 11 publications

References 27 publications

Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia

Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia

Diversity of Chromanol and Chromenol Structures and Functions: An Emerging Class of Anti-Inflammatory and Anti-Carcinogenic Agents

Free Radical Scavenging and Anticollagenase Properties of Sargachromanol I from Myagropsis myagroides

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