Inhibitory effect ofErythronium japonicumon the human breast cancer cell metastasis

Abstract

BACKGROUND/OBJECTIVESIn this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined.MATERIALS/METHODSCells were cultured with DMSO or with 50, 75, 100 or 250 µg/ml of Erythronium japonicum methanol or ethanol extract.RESULTSBoth methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts.CONCLUSIONSOur results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

Erythronium japonicum Alleviates Inflammatory Pain by Inhibiting MAPK Activation and by Suppressing NF-κB Activation via ERK/Nrf2/HO-1 Signaling Pathway

“…We performed an extract fractionation to identify the EJE component that most alleviated microglial activation and found it to be the butanol fraction, which suppressed LPS-induced microglial activation and largely increased HO-1 expression in BV2 cells. Erythronium japonicum consists of major components, including chlorogenic acid, caffeic acid, kaempferol, quercetin, astragalin, and isoquercitrin [4,46], which have preventive antioxidant and anti-inflammatory effects [16,[47][48][49][50]. Among these major components, the evidence has suggested that chlorogenic acid and caffeic acid could attenuate cognitive impairment and neurodegeneration-associated diseases [50].…”