Inhibitory Effect of Jing-Fang Powder n-Butanol Extract and Its Isolated Fraction D on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells

Rao, Zhili; Cao, Hai-Juan; Shi, Boyu; Liu, Xiaobo; Luo, Jie; Zeng, Nan

doi:10.1124/jpet.118.255893

Cited by 12 publications

(4 citation statements)

References 30 publications

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“…An interesting factor is that at the end of the last century, it was shown that the treatment of glia and cultured smooth muscle cells of the rat colon with IL-1β led to a significant increase in the activity of inducible nitric oxide synthase (iNOS) [ 270 ]. Similarly to the above, it was found that a decrease in IL-1β and IL-6 levels, as well as the suppression of mRNA expression of genes encoding these cytokines, correlated with the lower NO release levels in RAW264.7 cells inflammation model under the action of anti-inflammatory fraction A [ 300 ]. IL-6 was also known to be activated by prostaglandin E2 (PGE2) that is formed during the reaction catalyzed by cyclooxygenase-2 [ 301 ], in which the elevated levels are observed in inflammatory macrophages in various types of cancer [ 75 , 302 , 303 , 304 ].…”

Section: Role Of Inflammation In Cancer Progressionmentioning

confidence: 76%

Therapeutic Influence on Important Targets Associated with Chronic Inflammation and Oxidative Stress in Cancer Treatment

Neganova

Liu

Aleksandrova

et al. 2021

Cancers

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Chronic inflammation and oxidative stress are the interconnected pathological processes, which lead to cancer initiation and progression. The growing level of oxidative and inflammatory damage was shown to increase cancer severity and contribute to tumor spread. The overproduction of reactive oxygen species (ROS), which is associated with the reduced capacity of the endogenous cell defense mechanisms and/or metabolic imbalance, is the main contributor to oxidative stress. An abnormal level of ROS was defined as a predisposing factor for the cell transformation that could trigger pro-oncogenic signaling pathways, induce changes in gene expression, and facilitate accumulation of mutations, DNA damage, and genomic instability. Additionally, the activation of transcription factors caused by a prolonged oxidative stress, including NF-κB, p53, HIF1α, etc., leads to the expression of several genes responsible for inflammation. The resulting hyperactivation of inflammatory mediators, including TNFα, TGF-β, interleukins, and prostaglandins can contribute to the development of neoplasia. Pro-inflammatory cytokines were shown to trigger adaptive reactions and the acquisition of resistance by tumor cells to apoptosis, while promoting proliferation, invasion, and angiogenesis. Moreover, the chronic inflammatory response leads to the excessive production of free radicals, which further aggravate the initiated reactions. This review summarizes the recent data and progress in the discovery of mechanisms that associate oxidative stress and chronic inflammation with cancer onset and metastasis. In addition, the review provides insights for the development of therapeutic approaches and the discovery of natural substances that will be able to simultaneously inhibit several key oncological and inflammation-related targets.

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Section: Role Of Inflammation In Cancer Progressionmentioning

confidence: 76%

Therapeutic Influence on Important Targets Associated with Chronic Inflammation and Oxidative Stress in Cancer Treatment

Neganova

Liu

Aleksandrova

et al. 2021

Cancers

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“…Our sample sizes have remained relatively small, necessitating further exploration with larger cohorts to draw more robust conclusions. Moreover, the specific roles and contributions of CXCL5 and CXCL8 in the observed fold changes require a comprehensive investigation 30 …”

Section: Discussionmentioning

confidence: 99%

CXCL5/CXCL8 induces neutrophilic inflammation in peri‐implantitis

Park,

Kim

et al. 2024

J of Periodontal Research

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Objective and BackgroundThis research aimed to examine the role of C‐X‐C motif chemokine ligand 5 (CXCL5) and C‐X‐C motif chemokine ligand 8 (CXCL8; also known as IL‐8) in neutrophilic inflammation triggered by peri‐implantitis and to shed light on the underlying mechanisms that link them to the development of this condition.MaterialsThis study included 40 patients who visited the Department of Periodontology at Kyungpook University Dental Hospital. They were divided into two groups based on their condition: healthy implant (HI) group (n = 20) and peri‐implantitis (PI) group (n = 20). Biopsy samples of PI tissue were collected from the patients under local anesthesia. HI tissue was obtained using the same method during the second implant surgery. To construct libraries for control and test RNAs, the QuantSeq 3′ mRNA‐Seq Library Prep Kit (Lexogen, Inc., Austria) was used according to the manufacturer's instructions. Samples were pooled based on representative cytokines obtained from RNA sequencing results and subjected to Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Hematoxylin and eosin staining, and immunohistochemistry (IHC) analysis were performed to visually assess expression levels and analyze tissue histology. Student's t‐test was employed to conduct statistical analyses.ResultsInitially, heatmaps were used to examine gene expression variations between the HI and PI groups based on the results of RNA sequencing. Notably, among various cytokines, CXCL5 and CXCL8 had the highest expression levels in the PI group compared with the HI group, and they are known to be associated with inflammatory responses. In the gingival tissues, the expression of genes encoding cytokines such as interleukin (IL)‐1β, tumor necrosis factor‐alpha (TNF)‐α, interleukin (IL)‐6, and CXCL5/CXCL8 was assessed via RT‐qPCR. The mRNA expression level of CXCL5/CXCL8 significantly increased in the PI group compared with the HI group (p < .045). Contrarily, the mRNA expression level of interleukin 36 receptor antagonist (IL36RN) significantly decreased (p < .008). IHC enabled examination of the distribution and intensity of CXCL5/CXCL8 protein expression within the tissue samples. Specifically, increased levels of CXCL5/CXCL8 promote inflammatory responses, cellular proliferation, migration, and invasion within the peri‐implant tissues. These effects are mediated through the activation of the PI3K/Akt/NF‐κB signaling pathway.ConclusionsThis study found that the PI sites had higher gene expression level of CXCL8/CXCL5 in the soft tissue than HI sites, which could help achieve more accurate diagnosis and treatment planning.

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“…Jingfangsan is composed of a 1:1 combination of Schizonepetae Herba (Jingjie) and Saposhnikoviae Radix (Fangfeng), whose active ingredient in an N-butanol extract exerts anti-inflammatory and anti-allergic effects. 113,114 JFNE-A is a bioactive ingredient fractionated from this compound. According to a previous study, 115 JFNE-A treatment significantly alleviates LPS-induced pathological changes in the lungs of ALI mice.…”

Section: N-butanol Extract Of Jingfangsanmentioning

confidence: 99%

Research Progress on Autophagy Regulation by Active Ingredients of Traditional Chinese Medicine in the Treatment of Acute Lung Injury

Dong¹,

Yin²,

Hao³

et al. 2023

JIR

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Autophagy is a highly conserved process that maintains cell stability in eukaryotes, participates in the turnover of intracellular substances to maintain cell function, helps to resist pathogen invasion, and improves cell tolerance to environmental changes. Autophagy has been observed in many diseases, and the symptoms of these diseases are significantly improved by regulating autophagy. Autophagy is also involved in the development of lung diseases. Studies have shown that autophagy may play a beneficial or harmful role in acute lung injury (ALI), and ALI has been treated with traditional Chinese medicine designed to promote or inhibit autophagy. In this paper, the molecular mechanism and common pathways regulating autophagy and the relationship between autophagy and ALI are introduced, and the active ingredients of traditional Chinese medicine that improve ALI symptoms by regulating autophagy are summarized.

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Inhibitory Effect of Jing-Fang Powder n-Butanol Extract and Its Isolated Fraction D on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells

Cited by 12 publications

References 30 publications

Therapeutic Influence on Important Targets Associated with Chronic Inflammation and Oxidative Stress in Cancer Treatment

Therapeutic Influence on Important Targets Associated with Chronic Inflammation and Oxidative Stress in Cancer Treatment

CXCL5/CXCL8 induces neutrophilic inflammation in peri‐implantitis

Research Progress on Autophagy Regulation by Active Ingredients of Traditional Chinese Medicine in the Treatment of Acute Lung Injury

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