Inhibitory effect of liposome-encapsulated anthocyanin on melanogenesis in human melanocytes

Abstract

Context: Melanin plays an important role in preventing ultraviolet (UV) light-induced skin damage. Overexposure to UV radiation can lead to the formation of free radicals and trigger inflammation and hyperpigmentation of the skin. Anthocyanin can combat excessive free radicals in the body and can reduce the occurrence of inflammation. However, anthocyanin molecules are unstable and highly susceptible to degradation. Objective: The present study aims to elucidate the effects of liposome-capsulated anthocyanin (LCA) from Hibiscus sabdariffa Linn. on melanogenesis in human A375 melanocytes. Materials and methods: The effects of LCA with various doses (5-50 mg/mL) on cell viability, melanin content, tyrosinase activity, expression of the tyrosinase and microphthalmiaassociated transcription factor (MITF) were measured. Results: Anthocyanin exhibits scavenging activity on DPPH radical with the inhibitory rate of 11 and 24% at 20 and 50 mg/mL concentration treatment, respectively, and inhibitory effects on melanin production by 8, 14, 23 and 30% at 5, 10, 20 and 50 mg/mL concentration treatment, respectively. However, LCA has enhanced DPPH scavenging activity (64 and 76% at 20 and 50 mg/mL concentration treatment, respectively) and inhibitory effects against melanin synthesis (23, 35, 43 and 60% at 5, 10, 20 and 50 mg/mL concentration treatment, respectively). Moreover, anthocyanin-inhibited melanin synthesis occurs through the inhibition of tyrosinase enzymatic activity and suppression of the protein expression of tyrosinase and MITF. Discussion and conclusion: Liposome encapsulation increases the stabilization of anthocyanin and the inhibition of melanogenesis. Our findings indicate that LCA may be suitable as a photoprotective agent for the skin.

Oligomeric Procyanidin Nanoliposomes Prevent Melanogenesis and UV Radiation-Induced Skin Epithelial Cell (HFF-1) Damage

“…However, research on the role of encapsulated oligomeric proanthocyanidin in melanogenesis seems to be lacking for the present. However, in another published article, liposome-encapsulated anthocyanin was reported to enhance DPPH scavenging activity and inhibitory effects on melanin production in vitro [21], which partially supports our results that liposome-encapsulated flavonoid LSOPC might still preserve a better related bioactivity.…”

Ex Vivo and In Vivo Assessment of the Penetration of Topically Applied Anthocyanins Utilizing ATR-FTIR/PLS Regression Models and HPLC-PDA-MS

“…Catechins, EGCG, and quercetin have all been found to localize in the stratum corneum, viable epidermis and dermis [ 14 , 15 , 18 , 54 ], and these findings have been correlated with their health-promoting properties observed on the skin. Based on the evidence in the literature of similar health benefits of anthocyanins, it seems likely that they will also be preferentially located in these layers [ 6 , 20 , 55 , 56 , 57 ]. However, studies investigating their quantification in the epidermis and dermis, as well as the IC 50 values necessary to exhibit these benefits in vivo, need to be performed.…”

Anthocyanins, Vibrant Color Pigments, and Their Role in Skin Cancer Prevention

“…A study investigated the inhibitory effects of liposome-capsulated anthocyanin (LCA) isolated from Hibiscus sabadariffa Linn . on melanoma development in human A357 melanocytes [ 349 ]. The study demonstrated that anthocyanin with liposome encapsulation increased the stabilization of anthocyanin and the inhibition of melanogenesis.…”