Inhibitory effect of lysophosphatidylcholine on pancreatic lipase-mediated hydrolysis in lipid emulsion

Tsuzuki, Wakako; Ue, Akemi; Nagao, Akihiko; Endo, Miyuki; Abe, Masao

doi:10.1016/j.bbalip.2004.05.002

Cited by 25 publications

(20 citation statements)

References 25 publications

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“…Actually, the addition of taurodeoxycholic acid sodium salts or phospholipids was shown to restore the inhibitory effect of lysophospholipids on pancreatic lipase47. In the present work, we have observed an increased hydrolysis of TC8 in the duodenum as compared to the control.…”

Section: Discussionsupporting

confidence: 60%

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Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System

et al. 2008

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In the present study, we use a model gastro-intestinal system to study the influence of different food-grade surface-active molecules (Sn-2 monopalmitin, β-lactoglobulin, or lysophosphatodylcholine) on lipase activity. The interfacial activity of lipase and surfactants are assessed with the pendant drop technique, a commonly used tensiometry instrument. A mathematical model is adopted which enables quantitative determination of the composition of the water–oil interface as a function of bulk surfactant concentration in the water–oil mixtures. Our results show a decrease in gastric lipolysis when interfacially active molecules are incorporated into a food matrix. However, only the Sn-2 monopalmitin caused a systematic decrease in triglyceride hydrolysis throughout the gastro-intestinal tract. This effect is most likely due to exclusion of both lipase and triglyceride from the water–oil interface together with a probable saturation of the solubilization capacity of bile with monoglycerides. Addition of β-lactoglobulin or lysophopholipids increased the hydrolysis of fat after the gastric phase. These results can be attributed to an increasing interfacial area with lipase and substrate present at the interface. Otherwise, β-lactoglobulin, or lysophopholipids reduced fat hydrolysis in the stomach. From the mathematical modeling of the interface composition, we can conclude that Sn-2 monopalmitin can desorb lipase from the interface, which, together with exclusion of substrate from the interface, explains the gradually decreased triglyceride hydrolysis that occurs during the digestion. Our results provide a biophysics approach on lipolysis that can bring new insights into the problem of fat uptake.

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Section: Discussionsupporting

confidence: 60%

“…Previous reports47 have described that LysoPC causes an increase in the K m and a decrease in V max values for the interaction between pancreatic lipase and triacylglycerol. This indicates that the substrate affinity and catalytic activity is inhibited by LysoPC.…”

Section: Discussionmentioning

confidence: 81%

Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System

et al. 2008

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“…On the other hand, the increment in pancreatic lipase in HFD-fed rats may be due to its involvement in the TG absorption from the small intestine to the enterocytes and if somehow this initial movement of TG from the small intestinal lumen is blocked, hyperlipidemia can be prevented [27]. While, the improvement in its activity post berry therapy to HFD-fed rats may be related to berry can work as pancreatic lipase inhibitors whereby the digestion and absorption of fats can be suppressed while it increased the fecal lipid content in mice [30].…”

Section: Discussionmentioning

confidence: 99%

“…Serum γ-GGT activity was measured according to Persijnandvan der Slik [26] using QCA Diagnostic kits (Spain). Pancreatic lipase activity was performed according to previously described method [27].…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Berries Supplementation Modulates Body Weight and Metabolic Deteriorations in Obese Rats

Baz

Aly

2018

Asian J Pharm Clin Res

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Objective:The aim of the present work is to investigate the potential of purple (Morus rubra [MR]) and white (Morus alba [MA]) berry supplementation to modulate body weight (BW) and metabolic deteriorations in obese Wistar male rats.Methods: Seventy rats weighing 150.00±10 g (mean±standard deviation) were used; the rats were randomly divided into seven groups of 10 rats each. Obesity was induced by feeding animals high-fat diet (HFD), for 6 consecutive weeks then treated with the purple (MR) and white (MA) berry ethanol extracts for 6 weeks in a dose 300 mg/Kg BW. BW gain, food intake fecal fat content, food consumptions were determined. The fats and organs such as liver, kidney, and heart were also weighed. Lipid profiles included triglyceride, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol, as well as liverenzymes; alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, γ-glutamyltransferase (γ-GGT), and leptin were also determined.Results: Treatment of obese rats with white and purple berries showed significant reduction in BW reached to 23.68% with MR and 21.19% with MA berries compared to Orlistat (31.16%). Liver weight, visceral fat, and liver enzyme activities were also markedly ameliorated. In addition, lipid profile biomarkers were improved on treated obese rats with both Morus species. In addition, increase in fecal fat (250.73% and 256.25%, for MR and MA, respectively) was noticed while blood glucose, insulin, leptin levels, and pancreatic lipase activity was improved. In addition, supplementing obese rats with both Morus species extract significantly increase food intake reached 33.33% and 36.66%, for MR and MA, respectively. Conclusion:The ameliorating effect of both Morus extracts may be attributed to polyphenolic rich compounds which have antioxidant properties, suppressed lipid synthesis in hepatic tissue and inhibited pancreatic lipase activity which consequently recommended using as a promising antiobesity agents. El-Baz et al.different physiological biomarkers related metabolic changes including liver function enzyme activities, BW gain percentage, organ weight, food intake, visceral and fecal fats percentages, blood glucose, insulin, and leptin levels as well as pancreatic lipase activity. MATERIALS AND METHODS Collection of plant materialFresh fruits of white Morus alba (MA) and purple Morus rubra (MR) were collected in the Delta region, Egypt. The berries were selected according to uniformity of shape and color. The identification of the plant was confirmed by Therese Labib, Herbarium Section, El-Orman Botanical Garden, Giza, and Egypt. The fresh fruit samples were cleaned, stored in polyethylene bags and frozen at −20°C, till further use.

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“…The composition of the droplet surface layer plays a key role in vivo as it was shown that native human milk droplets of about 4 μm (three phospholipid layers) were more efficiently lipolyzed in the stomach of premature babies than small-sized droplets (0.5 μm) from a formula (single layer) 56. It was shown in vitro that specific classes of phospholipids will prevent pancreatic lipase activity,187 or enhance dramatically gastric lipase, pancreatic lipase, and BSSL activity in conditions that mimic physiology 188,189. Also, the dietary protein interaction within the droplet surface can improve or diminish the triglycerides lipolysis rates 190…”

Section: Future Developments For An Optimal Therapymentioning

confidence: 99%

Enzyme replacement therapy for pancreatic insufficiency: present and future

Fieker¹,

Philpott²,

Armand³

2011

CEG

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Pancreatic enzyme replacement therapy is currently the mainstay of treatment for nutrient malabsorption secondary to pancreatic insufficiency. This treatment is safe and has few side effects. Data demonstrate efficacy in reducing steatorrhea and fat malabsorption. Effective therapy has been limited by the ability to replicate the physiologic process of enzyme delivery to the appropriate site, in general the duodenum, at the appropriate time. The challenges include enzyme destruction in the stomach, lack of adequate mixing with the chyme in the duodenum, and failing to deliver and activate at the appropriate time. Treatment is begun when clinically significant malabsorption occurs resulting in steatorrhea and weight loss. Treatment failure is addressed in a sequential fashion. Current research is aimed at studying new enzymes and delivery systems to improve the efficiency of action in the duodenum along with developing better means to monitor therapy.

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Inhibitory effect of lysophosphatidylcholine on pancreatic lipase-mediated hydrolysis in lipid emulsion

Cited by 25 publications

References 25 publications

Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System

Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System

Berries Supplementation Modulates Body Weight and Metabolic Deteriorations in Obese Rats

Enzyme replacement therapy for pancreatic insufficiency: present and future

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