Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31

Abstract

The aim of the present study was to examine microRNA (miRNA or miR)-455-5p expression in esophageal squamous cell carcinoma (ESCC) at the tissue and cellular levels in order to elucidate its biological roles. A total of 60 patients with ESCC were enrolled in the present study and reverse transcription-quantitative polymerase chain reaction was used to measure the expression of miR-455-5p. ESCC Eca109 cells were transfected with miR-NC, miR-455-5p mimics or inhibitor and a Cell Counting Kit-8 assay was used to assess proliferation. To investigate the migration and invasion abilities of Eca109 cells, Transwell and Matrigel assays were performed. Western blotting was employed to measure Rab31 protein expression, while a rescue assay was utilized to study the biological roles of miR-455-5p and Rab31 in Eca109 cells. To determine whether Rab31 is a direct target of miR-455-5p, a dual luciferase reporter assay was performed. The results revealed that miR-455-5p expression was decreased in ESCC tissues and was negatively correlated with metastasis and pathogenesis. In vitro overexpression of miR-455-5p inhibited the proliferation, migration and invasion of ESCC Eca109 cells. Furthermore, miR-455-5p regulated the expression of Rab31 protein in Eca109 cells. Rab31 overexpression promoted the proliferation, migration and invasion of Eca109 cells. Luciferase reporter assay results revealed that miR-455-5p is able to bind with the 3′-untranslated region of Rab31 mRNA to regulate its expression. In summary, the results of the present study suggest that miR-455-5p expression is decreased in ESCC tissues and is miR-455-5p is negatively correlated with lymphatic metastasis and differentiation. As a tumor-suppressor gene, miR-455-5p inhibits the proliferation, migration and invasion of ESCC Eca109 cells by suppressing the expression of Rab31.

Emerging Role of Non-Coding RNAs in Esophageal Squamous Cell Carcinoma

“…Many such studies have been published so far, which reveal miRNAs as tumor suppressors or oncogenes (Table 1). For example, miR-146a, miR-133b, miR-106b-3p, miR-219-5p, miR-206, miR-384, miR-455-5p, miR-128, miR-145-3p/5p, miR-10b-3p, miR-874-3p, miR-10a, miR-365, miR-301a, miR-6775-3p, miR-139-5p, miR-516b, miR-449a-5p, miR-125b, miR-433-3p, miR-370, miR-133b, miR-30a-3p/5p, miR-34a, miR-196a, and miR-125b-5p have been shown to act as tumor suppressors by inhibiting ESCC cell proliferation, promoting apoptosis by directly targeting oncogenes, or antagonizing pro-cancer signaling pathways [29,33,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][54][55][56][57][58][60][61][62][63]110,133]. In contrast, pro-oncogenes such as miR-141, miR-21, miR-10b-3p, miR-424, miR-675-3p, miR-543, miR-135, miR-23b-3p, miR-502, miR-21-5p, and miR-548k have been reported to play contrasting roles in promoting cell proliferation or suppressing apoptosis in ESCC [47,[64][65][66][67][68][69][70][71][72][73].…”

“…At present, various miRNAs have been found to regulate EMT or tumor metastasis of ESCC. It has been reported that miR-106b-3p, miR-455-5p, and miR-128 function as tumor suppressors, and have suppressed EMT or metastasis in ESCC [33,43,44]. While miRNAs, such as miR-543, miR-23b-3p, miR-25, and miR-99b/let-7e/miR-125a play oncogenic roles in promoting EMT or metastasis during ESCC progression [68,70,109,113].…”

RAB31 drives extracellular vesicle fusion and cancer‐associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer

“…RAB31 expression has been confirmed in many colorectal cancer cell lines, which is consistent with clinical data that may indicate variations in morphology during the EMT process. The expression of RAB31 has been demonstrated to affect cell growth and motility in various cancer types such as esophageal squamous cell carcinoma, osteosarcoma and cervical cancer (Huang et al., 2022 ; Liu et al., 2018 ; Wu et al., 2020 ). Establishing a RAB31‐based model confirmed a correlation between the expression of E‐cadherin and mesenchymal‐related molecules (SNAI2, VIM and TWIST1) at the RNA and protein levels in colorectal cancer cells and RAB31 biological function.…”

Role of miR153 and miR455-5p Expression in Oral Squamous Cell Carcinoma Isolated from Plasma

“…In this study, the factors mentioned on sixty tumor tissues were compared with the adjacent healthy tissue and it was reported that miR455-5p can be thought as a miR suppressive tumor and plays a significant role in esophageal SCC by typically suppressing the expression of Rab31. It is important to note that soft tissues of the human oral cavity and esophagus are covered everywhere by a stratifying squamous epithelium (Liu et al, 2018).…”