Inhibitory effect of Morus australis leaf extract and its component iminosugars on intestinal carbohydrate-digesting enzymes

Qiao, Ying; Ito, Masaaki; Kimura, Toshiyuki; Ikeuchi, Takeaki; Takita, Teisuke; Yasukawa, Kiyoshi

doi:10.1016/j.jbiosc.2021.05.005

Cited by 11 publications

(13 citation statements)

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“…Measurement was carried out as described previously (Qiao et al., 2020, 2021). Briefly, 70 µl of 0.1 M phosphate buffer (pH 6.0), 10 µl of maltose, sucrose, or isomaltose solution, and 10 µl of M. australis fruit extract, C3G, C3R, or glucose solution were mixed and incubated at 37°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Measurement was carried out using as Glucoamylase and α‐glucosidase assay kit (Kikkoman Corporation, Noda, Japan) and an EnSight as described previously (Qiao et al., 2021). Briefly, the initial reaction rate for glucoamylase (starch hydrolase releasing β‐glucose from the non‐reducing ends of starch) activity (A) and other α‐glucosidase (aryl‐glucosides hydrolase releasing β‐glucose) activity (B) were determined, separately.…”

Section: Methodsmentioning

confidence: 99%

“…Measurement was carried out as described previously (Qiao et al, 2020(Qiao et al, , 2021. Briefly, 70 µl of 0.1 M phosphate buffer (pH 6.0), 10 µl of maltose, sucrose, or isomaltose solution, and 10 µl of M. australis fruit extract, C3G, C3R, or glucose solution were mixed and incubated at 37 • C for 10 min.…”

Section: Measurement Of Maltase Sucrase and Isomaltase Activitiesmentioning

confidence: 99%

“…α‐Amylase, in addition to α‐glucosidase, is a central enzyme involved in the digestion of carbohydrates. Iminosugars do not inhibit α‐amylase (Qiao et al., 2021). In contrast, the azadirachtins from Azadirachta indica , 4‐hydroxyisoleucine from Trigonella foenum‐graecum , and cinnamaldehyde from Cinnamomum sieboldii have been reported to have both α‐amylase and α‐glucosidase inhibitory activities (Governa et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Consumption of the M. australis leaf tea suppressed sucroseinduced increase in blood glucose and insulin levels in human (Kume et al, 2019(Kume et al, , 2021. We previously examined the inhibitory effects of powdered M. australis leaf extract and its component iminosugars 1-DNJ, fagomine, and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ) (Qiao et al, 2020(Qiao et al, , 2021, showing that 1-DNJ is a major inhibitor of α-glucosidase in the M. australis leaf extract. In addition to iminosugars, M. australis contains a range of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Qiao

Ikeda

Ito

et al. 2022

Journal of Food Science

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The inhibition of α‐amylase and α‐glucosidase are important for the maintenance of blood glucose level. Mammalian α‐glucosidase includes maltase–glucoamylase and sucrase–isomaltase complexes. In this study, we examined the inhibitory effects of Morus australis fruit extract and its components, that is, three iminosugars (1‐deoxynojirimycin [1‐DNJ], fagomine, and 2‐O‐α‐D‐galactopyranosyl deoxynojirimycin), two anthocyanins (cyanidin‐3‐glucoside and cyanidin‐3‐rutinoside), and glucose, against α‐amylase and α‐glucosidase. We also quantified the concentration of each component in M. australis fruit extract. The IC50 values of the fruit extracts of four M. australis subspecies were >10 mg/ml for α‐amylase, 1.1–1.7 mg/ml for maltase, 6.9–8.6 mg/ml for glucoamylase, 0.13–1.0 mg/ml for sucrase, and 0.46–1.4 mg/ml for isomaltase. When the IC50 value of each component and the concentration of each component in the fruit extract were taken into consideration, our results indicated that glucose are involved in the inhibition of α‐amylase, and 1‐DNJ and glucose are involved in the inhibition of α‐glucosidase. This is in contrast to that in M. australis leaf, neither anthocyanin nor glucose are contained, and 1‐DNJ is a main inhibitor. Practical Application It is widely accepted that inhibition of α‐amylase and α‐glucosidase is one of the strategies to treat type‐2 diabetes. Today, acarbose, miglitol, and voglibose are clinically used for this purpose. Our results that 1‐DNJ and anthocyanin are present in Morus australis fruit and are involved in the inhibition of α‐amylase and α‐glucosidase suggest that M. australis fruit is a healthy sweetener.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Maltase Sucrase and Isomaltase Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%