Inhibitory effect of Patrinia scabiosifolia Link on the development of atopic dermatitis-like lesions in human keratinocytes and NC/Nga mice

Cha, Kyung-Jae; Im, Mi Ae; Gu, Ayoung; Kim, Da Hye; Lee, Daye; Lee, Jeong Soo; Lee, Ji-Sook; Kim, In Sik

doi:10.1016/j.jep.2017.03.045

Cited by 14 publications

(10 citation statements)

References 33 publications

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“…IL‐17A was reported to be able to downregulate not only pro‐filaggrin and FLG expression but also proteins associated with cell adhesion between the keratinocytes ( Gutowska‐Owsiak et al, ) . In previous studies, FLG was downregulated in DNCB‐induced AD, while there exist comparatively few reports indicating that LOR and IVL were downregulated in DNCB‐induced AD yet (Cha et al, ). In the present study, repeated application of DNCB on the dorsal skin appeared to degradation of FLG, IVL, and LOR.…”

Section: Discussionmentioning

confidence: 94%

Pinus densiflora bark extract ameliorates 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in NC/Nga mice by regulating Th1/Th2 balance and skin barrier function

Lee

Jang

et al. 2018

Phytotherapy Research

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Korean red pine (Pinus densiflora) bark has been traditionally used in Korea and other parts of East Asia to relieve inflammatory diseases. Although many studies using P. densiflora bark have been reported, its effect on atopic dermatitis (AD) has not been elucidated. Thus, we investigated whether the P. densiflora bark extract (PBE) has potential to attenuate AD symptoms and elucidated the molecular mechanism. Oral administration of PBE to mice with 2,4-dinitrochlorobenzene (DNCB)-induced AD lessened dermatitis scores and scratching behavior and significantly reduced measures of epidermal thickness, infiltration of mast cells and eosinophils, levels of immunoglobulin E (IgE), and IgG /IgG ratio in serum. PBE not only inhibited IL-4, IL-5, and IL-13 but also increased IFN-γ in splenic production. Furthermore, PBE significantly suppressed mRNA expression of thymic stromal lymphopoietin and further downregulated the mRNA expression of Th2 and Th17 cytokines such as IL-4, IL-13, IL-17, IL-31, and TNF-α. In addition, the protein expressions of filaggrin, involucrin, and loricrin in lesional skin were recovered by PBE. These results suggest that PBE attenuates DNCB-induced AD via regulating Th1/Th2 balance and skin barrier function.

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Section: Discussionmentioning

confidence: 94%

Pinus densiflora bark extract ameliorates 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in NC/Nga mice by regulating Th1/Th2 balance and skin barrier function

Lee

Jang

et al. 2018

Phytotherapy Research

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“…defects in skin barrier function are an essential feature of the development of AD (17). Although filaggrin, loricrin, and involucrin are three key proteins in skin barrier Figure 5.…”

Section: Discussionmentioning

confidence: 99%

“…Both erK (erK1/2) and p38 MaPK have important roles in cytokine secretion and allergic diseases, including ad (21,22). c-Jun n-terminal kinase (JnK) is an important MaPK associated with filaggrin expression (17). Further studies are required to investigate the association between the alteration of skin barrier proteins and JnK activation.…”

Section: Discussionmentioning

confidence: 99%

Effect of S100A8 and S100A9 on expressions of cytokine and skin barrier protein in human keratinocytes

Kim

Lee

et al. 2019

Mol Med Report

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atopic dermatitis (ad) is an inflammatory skin disorder caused by immunological dysregulation and genetic factors. Whether the expression levels of cytokine and skin barrier protein were altered by S100 calcium binding protein a8 (S100a8) and S100a9 in human keratinocytic HacaT cells was examined in the present study. alterations of cytokine expression were examined by eliSa following treatment with S100a8/9 and various signal protein-specific inhibitors. Activation of the mitogen activated protein kinase (MaPK) pathway and nuclear factor (nF)-κB was evaluated by using western blotting and an nF-κB activity test, respectively. The expression levels of interleukin (il)-6, il-8 and monocyte chemoattractant protein-1 increased following treatment with S100a8 and S100a9, and the increase was significantly blocked by specific signaling pathway inhibitors, including toll-like receptor 4 inhibitor (Tlr4i), rottlerin, Pd98059, SB203580 and BaY-11-7085. extracellular signal-regulated kinase (erK) and p38 MaPK pathways were activated in a time-dependent manner following treatment with S100a8 and S100a9. Phosphorylation of erK and p38 MaPK were blocked by Tlr4i and rottlerin. S100a8 and S100a9 induced translocation of nF-κB in a time-dependent manner, and the activation of nF-κB was inhibited by Tlr4i, rottlerin, Pd98059 and SB203580. in addition, S100a8 and S100a9 decreased the expression of skin barrier proteins, filaggrin and loricrin. These results may help to elucidate the pathogenic mechanisms of ad and develop clinical strategies for controlling ad.

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“…In the development and aggravation of AD, the regulation of cytokine secretion, particularly the Th1/Th2 cytokines and chemokines, is a key process [18). TARC, MCP-1, and IL-8 have been reported as survival factors and pathogenic inducers of AD 19. IL-6 is secreted from T lymphocytes, macrophages, and eosinophils, and it plays an essential role in the transition from an acute inflammatory state to a chronic inflammatory state 20.…”

Section: Discussionmentioning

confidence: 99%

Poncirus Trifoliata (L.) Raf. Extract Inhibits the Development of Atopic Dermatitis-like Lesions in Human Keratinocytes and NC/Nga mice

Cha¹,

Kashif²,

Hong³

et al. 2019

Int. J. Med. Sci.

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This study investigated the anti-allergic effect of Poncirus trifoliata (L.) Raf. (PT) on human keratinocytic HaCaT cells in vitro and on 2,4‐dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like lesions in vivo. The release of TARC, MCP-1, IL-6 and IL‐8 is increased by IFN-γ and TNF-α in HaCaT cells, and PT extract suppressed the increased production of TARC, MCP-1, IL-6, and IL‐8. PT extract recovered the expression of filaggrin decreased by IFN-γ and TNF-α. in vivo experiment, PT administration decreased the skin severity score, thickening of the epidermis, movement of inflammatory cells into the dermis, and serum IgE level as compared to DNCB treatment. Moreover, the decrease of filaggrin and loricrin induced by DNCB treatment was recovered by PT administration. The levels of IL-4, IL-5, IL-13 and eotaxin in mouse splenocytes increased after treatment with concanavalin A, and the secretions of IL-4, IL-5, IL-13 and eotaxin were lower in the PT-treated group than in the DNCB group. These findings may indicate that PT is useful in drug development for the treatment of AD.

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Inhibitory effect of Patrinia scabiosifolia Link on the development of atopic dermatitis-like lesions in human keratinocytes and NC/Nga mice

Cited by 14 publications

References 33 publications

Pinus densiflora bark extract ameliorates 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in NC/Nga mice by regulating Th1/Th2 balance and skin barrier function

Pinus densiflora bark extract ameliorates 2,4‐dinitrochlorobenzene‐induced atopic dermatitis in NC/Nga mice by regulating Th1/Th2 balance and skin barrier function

Effect of S100A8 and S100A9 on expressions of cytokine and skin barrier protein in human keratinocytes

Poncirus Trifoliata (L.) Raf. Extract Inhibits the Development of Atopic Dermatitis-like Lesions in Human Keratinocytes and NC/Nga mice

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