Inhibitory effect of probiotic Bacillus spp. isolated from the digestive tract of Rhynchocypris Lagowskii on the adhesion of common pathogenic bacteria in the intestinal model

Wang, Jiajing; Wu, Zhenchao; Wang, Seng; Wang, Xin; Zhang, Dongming; Wang, Qiuju; Lin, Lili; Wang, Guiqin; Guo, Zhixin; Chen, Yuke

doi:10.1016/j.micpath.2022.105623

Cited by 10 publications

(7 citation statements)

References 64 publications

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“…Wang et al . [ 67 ] reported the hydrophobicity of bacterial isolates LSG1-1 and LSG2-1 isolated from the digestive tract of Rhynchocypris lagowskii , which had hydrophobicities of 73.44% and 77.81%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Mohammad et al [66] reported hydrophobicities of 59.41%, 52.09%, 74.51%, and 80.52% for isolates Lactobacillus musae SGMT17, Lactobacillus crustorum SGMT20, Lactobacillus mindensis SGMT22, and Leuconostoc mesenteroides U39, respectively. Wang et al [67] reported the hydrophobicity of bacterial isolates LSG1-1 and LSG2-1 isolated from the digestive tract of Rhynchocypris lagowskii, which had hydrophobicities of 73.44% and 77.81%, respectively.…”

Section: Hydrophobicity Of Labmentioning

confidence: 99%

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Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry

Srifani,

Mirnawati,

Marlida

et al. 2024

Vet World

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Background and Aim: In-feed antibiotics have been used as antibiotic growth promoters (AGPs) to enhance the genetic potential of poultry. However, the long-term use of AGPs is known to lead to bacterial resistance and antibiotic residues in poultry meat and eggs. To address these concerns, alternatives to AGPs are needed, one of which is probiotics, which can promote the health of livestock without having any negative effects. In vitro probiotic screening was performed to determine the ability of lactic acid bacteria (LAB) isolated from soymilk waste to be used as a probiotic for livestock. Materials and Methods: Four LAB isolates (designated F4, F6, F9, and F11) isolated from soymilk waste were used in this study. In vitro testing was performed on LAB isolates to determine their resistance to temperatures of 42°C, acidic pH, bile salts, hydrophobicity to the intestine, and ability to inhibit pathogenic bacteria. A promising isolate was identified using the 16S rRNA gene. Results: All LAB isolates used in this study have the potential to be used as probiotics. On the basis of the results of in vitro testing, all isolates showed resistance to temperatures of 42°C and low pH (2.5) for 3 h (79.87%–94.44%) and 6 h (76.29%– 83.39%), respectively. The survival rate at a bile salt concentration of 0.3% ranged from 73.24% to 90.39%, whereas the survival rate at a bile salt concentration of 0.5% ranged from 56.28% to 81.96%. All isolates showed the ability to attach and colonize the digestive tract with a hydrophobicity of 87.58%–91.88%. Inhibitory zones of LAB against pathogens ranged from 4.80–15.15 mm against Staphylococcus aureus, 8.85–14.50 mm against Salmonella enteritidis, and 6.75–22.25 mm against Escherichia coli. Although all isolates showed good ability as probiotics, isolate F4 showed the best probiotic ability. This isolate was identified as Lactobacillus casei strain T22 (JQ412731.1) using the 16S rRNA gene. Conclusion: All isolates in this study have the potential to be used as probiotics. However, isolate F4 has the best probiotic properties and is considered to be the most promising novel probiotic for poultry. Keywords: lactic acid bacteria, poultry, probiotic.

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Section: Discussionmentioning

confidence: 99%

Section: Hydrophobicity Of Labmentioning

confidence: 99%

Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry

Srifani,

Mirnawati,

Marlida

et al. 2024

Vet World

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“…The antimicrobial activity of isolated strained was tested following previous study (Wang et al, 2022). Indicator strains, including Vibrio harveyi, Vibrio alginolyticus, Pseudomonas plecoglossicida, Aeromonas hydrophila, and Edwardsiella tarda The strains used for the antimicrobial test were taken from growth stationary phase cells and cultured three times in passages, with their optical density at 600 nm (OD 600 ) adjusted to 0.3.…”

Section: Antimicrobial Activity Of Isolated Strainsmentioning

confidence: 99%

“…Bacterial agglutination ability was determined following previously published methods (Wang et al, 2022). In brief, bacterial cultures were incubated at 37°C for 16-18 h, then centrifuged at 4 500 rpm for 10 min and washed twice with sterile phosphate-buffered saline (PBS, Biosharp, China).…”

Section: Bacterial Culture and In Vitro Mucus Preparationmentioning

confidence: 99%

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Isolation and characterization of probiotic Lysinibacillus species from the gastrointestinal tract of large yellow croaker (Larimichthys crocea)

Yao,

Wang,

Lin

et al. 2024

Front. Mar. Sci.

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The objective of this study was to identify effective strategies for disease prevention and control that circumvent antimicrobial resistance (AMR) issues, thereby facilitating sustainable aquaculture practices. This research focused on isolating probiotic Bacillus strains from the intestinal tract of healthy large yellow croaker (Larimichthys crocea). Among the 11 strains tested, four demonstrated protease and lipase activities. Further testing identified one strain, Lysinibacillus sp. (LYD11), with strong inhibitory activity against Vibrio harveyi and Vibrio alginolyticus, two major pathogens of the large yellow croaker. The screened strains showed more than 45% co-aggregation with five pathogens and were capable of inhibiting pathogen colonization through competition, repulsion, and substitution adhesion of 20.56 to 48.45%, 40.37 to 75.62% and 55.51 to 85.30%, respectively. The hydrophobicity and self-aggregation rates of LYD11 were 82.73% and 49.47%, respectively, indicating a potent ability for adhesion and colonization within the fish intestine. LYD11 demonstrated robust growth under the NaCl 0.5–3.5% and pH range of 6–8 and was unaffected by the gastrointestinal environment. Safety evaluations at high concentrations in grouper, in both injected and fed groups, indicated no adverse effects, as evidenced by the absence of morbidity or mortality. Consequently, Lysinibacillus sp. LYD11 emerges as a potential probiotic candidate for the prevention and control of bacterial diseases in the large yellow croaker.

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The use of microbes in culture of harpacticoid copepod (Tigriopus japonicus Mori) for suppression of Vibrio and enhancement of population growth, enzyme activity and microbial longevity

Sahandi

Sorgeloos²,

Xiao³

et al. 2023

Aquaculture

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Inhibitory effect of probiotic Bacillus spp. isolated from the digestive tract of Rhynchocypris Lagowskii on the adhesion of common pathogenic bacteria in the intestinal model

Cited by 10 publications

References 64 publications

Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry

Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry

Isolation and characterization of probiotic Lysinibacillus species from the gastrointestinal tract of large yellow croaker (Larimichthys crocea)

The use of microbes in culture of harpacticoid copepod (Tigriopus japonicus Mori) for suppression of Vibrio and enhancement of population growth, enzyme activity and microbial longevity

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