Inhibitory Effect of Quercetin on Tryptase and MCP-1 Chemokine Release, and Histidine Decarboxylase mRNA Transcription by Human Mast Cell-1 Cell Line

Abstract

Mast cells are important in reactions of allergic disease and are also involved in a variety of neuroinflammatory diseases. Mast cells can be immunologically activated by IgE through their Fc receptors, as well as by neuropeptides and cytokines to secrete mediators. Here we used a human mast cell-1 (HMC-1) cell line cultured and treated with a physiological activator, anti-IgE, and a nonphysiological activator, calcium ionophore A23187, for tryptase and MCP-1 generation and transcription of histidine decarboxylase. We used quercetin, a potent antioxidant, cytoprotective and anti-inflammatory compound capable of inhibiting histamine and some cytokines released from several cell types, as an inhibitor of immunological and nonimmunological stimulus for mast cells. In this study quercetin inhibits, in a dose-response manner, tryptase and MCP-1. Moreover, using RT-PCR quercetin inhibited the transcription of histidine decarboxylase, the rate-limiting enzyme responsible for the generation of histamine from histidine, and MCP-1. Our data suggest that quercetin is an important and good candidate for reducing the release of pro-inflammatory mast cell mediators activated by physiological and nonphysiological stimulators.

A randomized, double-blind, placebo-controlled study to evaluate the effect of EFF1001, anActinidia arguta(hardy kiwi) preparation, on CADESI score and pruritus in dogs with mild to moderate atopic dermatitis

“…An unpublished analysis by Efficas, Inc. of the phenolic constituents of EFF1001 by HPLC‐UV revealed the presence of the ubiquitous flavonoids quercetin, isorhamnetin, kaempferol, in approximate amounts ranging from 20 to 80 μg/mL. There are numerous reports in the literature on the anti‐inflammatory effects of these and similar flavonoids; 9–12 however, the degree to which these compounds contribute to the overall activity of EFF1001 is unknown.…”

Mast cell lines HMC‐1 and LAD2 in comparison with mature human skin mast cells – drastically reduced levels of tryptase and chymase in mast cell lines

“…The cell lines have been characterized one at a time, but their direct relation to normal human tissue MC has remained unknown. Nevertheless, both cell lines are extensively used as surrogates for non-transformed primary human MC (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24).…”

IL-34 a Newly Discovered Cytokine

“…They are multifunctional proteins which have a role in both innate and adaptive immune responses (8-13) with an effect on proliferation, differentiation, inhibition, apoptosis and chemoattraction (14-16). In addition, cytokines are involved in and mediate many different diseases (17-18).The newly-characterized Interleukin-34 (IL-34) plays a central role in innate immunity and adaptive immunity, and is an important molecule for the communication between cells, tissues, and organs.Recently it has been reported that interleukin-34 (lL-34) stimulates monocyte viability but does not affect responses in a wide spectrum ofother assays (20)(21)(22)(23)(24)(25). In both in vitro and in vivo studies, IL-34 directly modulates the number and function of monocytes and regulates myeloid cell growth and differentiation.…”

“…Recently it has been reported that interleukin-34 (lL-34) stimulates monocyte viability but does not affect responses in a wide spectrum ofother assays (20)(21)(22)(23)(24)(25). In both in vitro and in vivo studies, IL-34 directly modulates the number and function of monocytes and regulates myeloid cell growth and differentiation.…”