Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase

Hao, Huifeng; Liu, Limei; Liu, Yuying; Liu, Juan; Li, Yan; Pan, Chun‐Shui; Wang, Mingxia; Wang, Chuan-She; Fan, Jing‐Yu; Gao, Yuansheng; Han, Jing‐Yan

doi:10.1016/j.jep.2014.04.050

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…The study on the vasodilatory potential of Rhy has received increasing attention in view of its hypotensive effect. It's well-documented that Rhy exerted vasodilation effect via blocking calcium signaling in the smooth muscle cells (Zhang et al, 2004 ; Li et al, 2013 ; Hao et al, 2014 ), however, the protective action of Rhy in endothelial cells has not been reported. Kuramochi and colleagues reported that a crude aqueous extract of U. rhynchophylla (Miq.)…”

Section: Discussionmentioning

confidence: 99%

“…Indomethacin (10 −5 M) was prepared with equimolar Na 2 CO 3 in distilled water. Rhy was dissolved in hydrochloric acid (0.1 M) to a concentration of 0.1 M, and diluted in distilled water for further usage (Hao et al, 2014 ). SNP, ACh, and PE were dissolved in distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Rhynchophylline (Rhy), a pharmacologically active substance isolated from UR , is widely used for the treatment of hypertension (Sutter and Wang, 1993 ; Zhou and Zhou, 2010 ; Ndagijimana et al, 2013 ). Antihypertensive action of Rhy is observed in a number of animal models (Zhang et al, 2010 ; Zhou and Zhou, 2010 ), and has been attributed to its possible vasodilatory action via inhibiting L-type Ca 2+ channel and/or decreasing calcium sensitivity in the smooth muscle cells (Zhang et al, 2004 ; Li et al, 2013 ; Hao et al, 2014 ). However, it remains unclear whether Rhy could ameliorate endothelial dysfunction in hypertension.…”

Section: Introductionmentioning

confidence: 99%

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Rhynchophylline Ameliorates Endothelial Dysfunction via Src-PI3K/Akt-eNOS Cascade in the Cultured Intrarenal Arteries of Spontaneous Hypertensive Rats

et al. 2017

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Objectives: To examine the protective effect of Rhynchophylline (Rhy) on vascular endothelial function in spontaneous hypertensive rats (SHRs) and the underlying mechanism.Methods: Intrarenal arteries of SHRs and Wistar rats were suspended in myograph for force measurement. Expression and phosphorylation of endothelial nitric oxide (NO) synthase (eNOS), Akt, and Src kinase (Src) were examined by Western blotting. NO production was assayed by ELISA.Results: Rhy time- and concentration-dependently improved endothelium-dependent relaxation in the renal arteries from SHRs, but had no effect on endothelium-independent relaxation in SHR renal arteries. Wortmannin (an inhibitor of phosphatidylinositol 3-kinase) or PP2 (an inhibitor of Src) inhibited the improvement of relaxation in response to acetylcholine by 12 h-incubation with 300 μM Rhy. Western blot analysis revealed that Rhy elevated phosphorylations of eNOS, Akt, and Src in SHR renal arteries. Moreover, wortmannin reversed the increased phosphorylations of Akt and eNOS induced by Rhy, but did not affect the phosphorylation of Src. Furthermore, the enhanced phosphorylations of eNOS, Akt, and Src were blunted by PP2. Importantly, Rhy increased NO production and this effect was blocked by inhibition of Src or PI3K/Akt.Conclusion: The present study provides evidences for the first time that Rhy ameliorates endothelial dysfunction in SHRs through the activation of Src-PI3K/Akt-eNOS signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rhynchophylline Ameliorates Endothelial Dysfunction via Src-PI3K/Akt-eNOS Cascade in the Cultured Intrarenal Arteries of Spontaneous Hypertensive Rats

et al. 2017

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“…Anti-hypertensive activities of Rhy have been reported in many animal models [ 42 ] and are attributed to its vasodilatory effect by decreasing calcium sensitivity in smooth muscles or through inhibition of L-type calcium channels [ 96 , 97 ]. Hypertension is a cardiovascular syndrome that causes functional and structural changes in the heart and blood vessels.…”

Section: Rhynchophylline In Atherosclerosis and Other Cardiovascular Diseasesmentioning

confidence: 99%

Recent Advances in the Anti-Inflammatory Activity of Plant-Derived Alkaloid Rhynchophylline in Neurological and Cardiovascular Diseases

Geetha

Ramachandran

2021

Pharmaceutics

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Rhynchophylline (Rhy) is a plant-derived indole alkaloid isolated from Uncaria species. Both the plant and the alkaloid possess numerous protective properties such as anti-inflammatory, neuroprotective, anti-hypertensive, anti-rhythmic, and sedative effects. Several studies support the significance of the anti-inflammatory activity of the plant as an underlying mechanism for most of the pharmacological activities of the alkaloid. Rhy is effective in protecting both the central nervous system and cardiovascular system. Cerebro-cardiovascular disease primarily occurs due to changes in lifestyle habits. Many previous studies have highlighted the significance of Rhy in modulating calcium channels and potassium channels, thereby protecting the brain from neurodegenerative diseases and related effects. Rhy also has anticoagulation and anti-platelet aggregation activity. Although Rhy has displayed its role in protecting the cardiovascular system, very little is explored about its intervention in early atherosclerosis. Extensive studies are required to understand the cardioprotective effects of Rhye. This review summarized and discussed the various pharmacological effects of Rhy in neuro- and cardioprotection and in particular the relevance of Rhy in preventing early atherosclerosis using Rhy-loaded nanoparticles.

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“…due to cerebral ischemia and hypoxia, the intimate connection between cerebral vascular endothelial cells is destroyed, the microvascular basement membrane is damaged and the permeability of the vascular wall is increased, resulting in punctiform hemorrhage and brain edema (17,18). additionally, the vasomotion of cerebral arterioles is inhibited and the blood cannot reach the capillaries controlled by the arterioles, leading to the injury and necrosis of the neurons (19,20). Moreover, the structure of small blood vessels is damaged, which provokes cerebral arterial stenosis or occlusion and a decrease in collateral circulation, thereby further aggravating the disturbance in microcirculation (21).…”

Section: Introductionmentioning

confidence: 99%

CPCGI confers neuroprotection by enhancing blood circulation and neurological function in cerebral ischemia/reperfusion rats

Miao

Wang

et al. 2019

Mol Med Report

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The current study used a rat middle cerebral artery occlusion (Mcao) model with the aim to explore the effects of compound porcine cerebroside and ganglioside injection (cPcGi) on brain ischemia/reperfusion injury in rats. improvement in the infarct-side microcirculation and the overall recovery of neurological function were detected by triphenyltetrazolium chloride staining, laser speckle blood flow monitoring, latex perfusion, immunofluorescence and immunoblotting. The results revealed that administration of cPcGi for 7 consecutive days following ischemic stroke contributed to the recovery of neurological function and the reduction of cerebral infarct volume in rats. Blood flow monitoring results demonstrated that the administration of cPcGi effectively promoted cerebral blood flow following stroke, and contributed to the protection of the ischemic side blood vessels. in addition, cPcGi treatment increased the numbers of new blood vessels in the peripheral ischemic region, and upregulated the expression levels of vascular endothelial growth factor, angiopoietin 1 and its receptor TEK receptor tyrosine kinase, fibroblast growth factor and Wnt signaling pathway-associated proteins. Taken together, the present results indicated that cPcGi improved the blood circulation and neurological function following cerebral ischemia/reperfusion in rats.

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Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase

Cited by 13 publications

References 33 publications

Rhynchophylline Ameliorates Endothelial Dysfunction via Src-PI3K/Akt-eNOS Cascade in the Cultured Intrarenal Arteries of Spontaneous Hypertensive Rats

Rhynchophylline Ameliorates Endothelial Dysfunction via Src-PI3K/Akt-eNOS Cascade in the Cultured Intrarenal Arteries of Spontaneous Hypertensive Rats

Recent Advances in the Anti-Inflammatory Activity of Plant-Derived Alkaloid Rhynchophylline in Neurological and Cardiovascular Diseases

CPCGI confers neuroprotection by enhancing blood circulation and neurological function in cerebral ischemia/reperfusion rats

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