Inhibitory effect of salicylhydroxamic acid on theobroxide-induced potato tuber formation

Gao, Xiaoge; Yang, Qing; Minami, Chisato; Matsuura, Hideyuki; Kimura, Atsuo; Yoshihara, Toshio

doi:10.1016/s0168-9452(03)00280-2

Cited by 26 publications

(39 citation statements)

References 23 publications

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“…LOX activity was determined as previously described (Gao et al 2003). The leaves of potato and morning glory were harvested at specified times and frozen immediately at À80°C.…”

Section: Measurement Of Lox Activitymentioning

confidence: 99%

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Relation Between Environmental Factors and the LOX Activities Upon Potato Tuber Formation and Flower-bud Formation in Morning Glory

et al. 2005

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Life cycles of plants including tuberization and flowering are strongly related to environmental factors such as photoperiod and temperature. Theobroxide induces potato tuber formation and flower bud formation of morning glory under non-inductive conditions and stimulates the activity of lipoxygenase (LOX). In this study, to understand the LOX activity more systematically, the relationships between LOX activity and light and temperature, which effects potato tuber and flower-bud formation, have been investigated. The results showed that LOX activity in morning glory was greatly enhanced up to 30 min and then declined after switching from the light to the dark condition, while the activity did not vary when switching from the dark to the light condition. In addition, the temperature profile of measured LOX activity in the potato and morning glory plants was nearly consistent with the time taken to form potato tubers and flower buds in morning glory, respectively, at different growing temperatures. These results strongly suggest that LOX activity is directly connected with light and temperature to regulate the formation of tubers and flowerbuds.

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“…LOX activity was determined as previously described (Gao et al 2003). The leaves of potato and morning glory were harvested at specified times and frozen immediately at À80°C.…”

Section: Measurement Of Lox Activitymentioning

confidence: 99%

“…In our previous study, theobroxide treatment of potato and morning glory plants increased lipoxygenase (LOX, EC 1.13.11.12) activities as well as endogenous jasmonic acid (JA) and tuberonic acid (TA) levels (Gao et al 2003).…”

Section: Introductionmentioning

confidence: 95%

Relation Between Environmental Factors and the LOX Activities Upon Potato Tuber Formation and Flower-bud Formation in Morning Glory

et al. 2005

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“…This signaling molecule led to an increase in LOX activity as well as endogenous JA content (Figure 1). Time course experiments (Figure 2) showed that the LOX activity was clearly detectable in the wheat callus after 6 h exposed to MeJA and increased continuously for the next 18 h. Furthermore, SHAM, which is an efficient inhibitor of JA biosynthesis, could suppress LOX activity and gene expression (Kn fel et al, ӧ 1984;Gao et al, 2003;Yang et al, 2011). It was reported that SHAM inhibit LOX activity by 90% and prevent the synthesis of the vegetative storage protein mRNA (Macrì et al, 1994;Staswick et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Ma¹,

Wang²,

Sun³

et al. 2015

JAS

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Drought stress is a major challenge in agriculture, causing severe loss of crops throughout the world. Methyl jasmonate (MeJA) regulates a variety of plant developmental process and responds to many biotic and abiotic stresses. To explore the physiological mechanisms of drought stress mitigated by exogenous MeJA, we subjected callus of wheat (Triticum aestivum L.) to drought induced medium after pretreatment with 10 ml sterile solution that contained the following singly or in combinations: MeJA (0, 0.25, and 2.5µM) and the lipoxygenase (LOX) inhibitor salicylhydroxamic acid (SHAM; 0 and 0.5 mM) for 24 hours. The endogenous jasmonic acid (JA) content, activity of LOX, hydrogen peroxide (H 2 O 2 ) content, antioxidant enzymes activities, levels of reactive oxygen species (ROS), content of malondialdehyde (MDA), and cell viability were measured. The results showed that exogenous MeJA induced the activity of LOX and resulted in rapid increase in endogenous JA levels in a concentration-dependent manner; JA declined gradually after reaching its maximum. The level of H 2 O 2 increased with increases in LOX activity and endogenous JA level, which are involved in the octadecanoid signaling pathway. However, SHAM inhibited the LOX activity, the endogenous JA level, and the accumulation of H 2 O 2 in wheat callus. In addition, the activities of several antioxidant enzymes increased after MeJA-pretreatment, but this effect was also inhibited by SHAM. The cumulative results suggest that H 2 O 2 was generated through the octadecanoid signaling pathway, and some antioxidant defense genes were provoked by the accumulation of H 2 O 2 . Therefore, we inferred that H 2 O 2 might act as a signaling molecule for the MeJA-induced antioxidant defense. Drought stress can be improved by MeJA-pretreatment, leading to decrease in endogenous MDA and ROS contents, which are normally induced by drought conditions. Both drought stress and exogenous MeJA-pretreatment increased in LOX activity, endogenous JA level, and antioxidant enzymes. Combinatorial treatments showed an apparent synergistic effect on the activities of these antioxidant enzymes and resulted in improved cell viability. These results confirmed the hypothesis that H 2 O 2 acts as a signaling molecule for the MeJA-induced antioxidant defense and could alleviate the negative effects of drought stress on wheat callus.

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“…26,27) Interestingly, theobroxide can also suppress stem elongation in morning glory, and JA is positively involved in this suppression.…”

Section: Discussionmentioning

confidence: 99%

Expression of Allene Oxide Cyclase fromPharbitis nilupon Theobroxide Treatment

Kong

Abe

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Inhibitory effect of salicylhydroxamic acid on theobroxide-induced potato tuber formation

Cited by 26 publications

References 23 publications

Relation Between Environmental Factors and the LOX Activities Upon Potato Tuber Formation and Flower-bud Formation in Morning Glory

Relation Between Environmental Factors and the LOX Activities Upon Potato Tuber Formation and Flower-bud Formation in Morning Glory

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Expression of Allene Oxide Cyclase fromPharbitis nilupon Theobroxide Treatment

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