Inhibitory effect of the TSG-6 on the BMP-4/Smad signaling pathway and odonto/osteogenic differentiation of dental pulp stem cells

Wang, Ying; Yuan, Shuai; Sun, Jingjing; Gong, Yuping; Liu, Sirui; Guo, Runying; He, Wei; Peng, Kang; Li, Rui

doi:10.1016/j.biopha.2020.110266

Cited by 14 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process is deemed to be regulated by various pathways, though not completely elucidated. The BMP-4/Smad signaling pathway is essential for the osteogenic differentiation of DPSCs, which can be suppressed by tumor necrosis factor-inducible protein-6 (TSG-6) (Wang Y. et al, 2020). The impaired osteogenic capacity of DPSCs in the inflammatory microenvironment can be rescued by WNT4 overexpression, which subsequently may function by affecting JNK signaling pathways (Zhong et al, 2019(Zhong et al, , 2020.…”

Section: Osteogenesismentioning

confidence: 99%

Dental-Derived Mesenchymal Stem Cells: State of the Art

Ouchi

Cao

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) could be identified in mammalian teeth. Currently, dental-derived MSCs (DMSCs) has become a collective term for all the MSCs isolated from dental pulp, periodontal ligament, dental follicle, apical papilla, and even gingiva. These DMSCs possess similar multipotent potential as bone marrow-derived MSCs, including differentiation into cells that have the characteristics of odontoblasts, cementoblasts, osteoblasts, chondrocytes, myocytes, epithelial cells, neural cells, hepatocytes, and adipocytes. Besides, DMSCs also have powerful immunomodulatory functions, which enable them to orchestrate the surrounding immune microenvironment. These properties enable DMSCs to have a promising approach in injury repair, tissue regeneration, and treatment of various diseases. This review outlines the most recent advances in DMSCs’ functions and applications and enlightens how these advances are paving the path for DMSC-based therapies.

show abstract

Section: Osteogenesismentioning

confidence: 99%

Dental-Derived Mesenchymal Stem Cells: State of the Art

Ouchi

Cao

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…The BMP-4/Smad signaling pathway is essential for the osteogenic differentiation of hDPSCs, which can be suppressed by the tumor necrosis factor-inducible protein-6 (TSG-6) [ 139 ]. The osteogenic potency of hDPSCs is also regulated by WNT, which may subsequently affect JNK signaling pathways [ 140 ].…”

Section: Dental Mscs-cm In Tissue Regenerationmentioning

confidence: 99%

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Bär

Lis-Nawara

Grelewski

2021

IJMS

View full text Add to dashboard Cite

The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting of various biomolecules, is undergoing intense research. Despite promising in vitro and in vivo studies, most DSC secretome-based therapies have not been implemented in human medicine because the paracrine effect of the bioactive factors secreted by human dental pulp stem cells (hDPSCs) and human exfoliated deciduous teeth (SHEDs) is not completely understood. In this review, we outline the current data on the hDPSC- and SHED-derived secretome as a potential candidate in the regeneration of bone, cartilage, and nerve tissue. Published reports demonstrate that the dental MSC-derived secretome/conditional medium may be effective in treating neurodegenerative diseases, neural injuries, cartilage defects, and repairing bone by regulating neuroprotective, anti-inflammatory, antiapoptotic, and angiogenic processes through secretome paracrine mechanisms. Dental MSC-secretomes, similarly to the bone marrow MSC-secretome activate molecular and cellular mechanisms, which determine the effectiveness of cell-free therapy. Many reports emphasize that dental MSC-derived secretomes have potential application in tissue-regenerating therapy due to their multidirectional paracrine effect observed in the therapy of many different injured tissues.

show abstract

“…Therefore, the ability of cells to migrate is beneficial to tissue reparation [ 26 ]. Second, DSPP and DMP-1 were two classic odontogenic differentiation markers as members of the small integrin-binding ligand N-linked glycoproteins family (SIBLINGs) [ 9 , 27 , 28 , 29 ]. Our results showed that 10 μM icariin enhanced mRNA and protein expression levels of DSPP and DMP-1 known to play a vital role in the progression and formation of pulp-dentin complex [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Odontogenic Effect of Icariin on the Human Dental Pulp Cells

et al. 2022

View full text Add to dashboard Cite

Background and Objectives: Human dental pulp cells (HDPCs) can be used for dentin regeneration due to its odontogenic differentiation property. Icariin can induce osteogenic differentiation of stem cells. However, its potential to induce odontogenic differentiation of HDPCs remains unclear. Thus, the aim of this study was to evaluate the capacity of icariin to induce odontogenic differentiation of HDPCs and investigate the underlying molecular mechanism. Materials and Methods: Cell viability assay was used to detect the cytotoxicity of icariin to HDPCs. Effect of icariin on HDPCs chemotaxis was measured by scratch migration assay. The mineralized and odontogenic differentiation of HDPCs was assessed by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining, real-time PCR, and Western blot of dentin matrix protein 1 (DMP 1) and dentin sialophosphoprotein (DSPP). In addition, Mitogen-activated protein kinase (MAPK) signaling pathway of icariin-induced biomineralization was investigated by Western blot. Results: Cells treated with icariin at all concentrations tested maintained viability, indicating that icariin was biocompatible. Icariin accelerated HDPCs chemotaxis (p < 0.05). Expression levels of related odontogenic markers were increased in the presence of icariin (p < 0.05). Icariin-induced odontogenic differentiation occurred via activation of the MAPK signaling pathway. Furthermore, MAPK inhibitors suppressed expression levels of DSPP and DMP 1 protein, ALP activity, and mineralization of HDPCs. Conclusions: Icariin can upregulate odontogenic differentiation of HDPCs by triggering the MAPK signaling pathway.

show abstract

Inhibitory effect of the TSG-6 on the BMP-4/Smad signaling pathway and odonto/osteogenic differentiation of dental pulp stem cells

Cited by 14 publications

References 43 publications

Dental-Derived Mesenchymal Stem Cells: State of the Art

Dental-Derived Mesenchymal Stem Cells: State of the Art

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Odontogenic Effect of Icariin on the Human Dental Pulp Cells

Contact Info

Product

Resources

About