Inhibitory Effect of Used Litter on Salmonella typhimurium Transmission in the Chicken

Olesiuk, Olga M.; Snoeyenbos, G. H.; Smyser, C. F.

doi:10.2307/1588396

Cited by 33 publications

(15 citation statements)

References 6 publications

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“…In pen trials, used litter contaminated with S. Typhimurium did not cause transfer to newly placed chickens and the New Litter, N1 and N2 Salmonella in the litter could not be detected in 3-5 days (Olesiuk, Snoeyenbos, & Smyser, 1971). It has been shown that Salmonella does not persist as long in old poultry litter as in fresh litter (Fanelli, Sadler, & Brownell, 1970).…”

Section: Discussionmentioning

confidence: 97%

Impact of the Australian litter re-use practice on Salmonella in the broiler farming environment

Chinivasagam¹,

Tran²,

Blackall³

2012

Food Research International

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Section: Discussionmentioning

confidence: 97%

Impact of the Australian litter re-use practice on Salmonella in the broiler farming environment

Chinivasagam¹,

Tran²,

Blackall³

2012

Food Research International

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“…Recognizing the importance of the litter environment, especially in commercial broiler houses with high stocking densities, several research groups have investigated how the physiochemical properties of broiler litter affect the growth and health of the birds (15) and the microbiota in the litter, using either cultivationbased or molecular biology techniques (22,23,33). Early studies focused on the effect of litter on enteric pathogens, including Salmonella (35,12,28) and cellulitis-causing Escherichia coli (38). From analyzing 16S rRNA gene clone libraries, Lu et al (23) showed that in broiler litter at various stages of reuse, low-GϩC Gram-positive bacteria predominated.…”

mentioning

confidence: 99%

Interrelations between the Microbiotas in the Litter and in the Intestines of Commercial Broiler Chickens

Cressman

Nelson

et al. 2010

Appl Environ Microbiol

157

118

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The intestinal microbiota of broiler chickens and the microbiota in the litter have been well studied, but the interactions between these two microbiotas remain to be determined. Therefore, we examined their reciprocal effects by analyzing the intestinal microbiotas of broilers reared on fresh pine shavings versus reused litter, as well as the litter microbiota over a 6-week cycle. Composite ileal mucosal and cecal luminal samples from birds (n ‫؍‬ 10) reared with both litter conditions (fresh versus reused) were collected at 7, 14, 21, and 42 days of age. Litter samples were also collected at days 7, 14, 21, and 42. The microbiotas were profiled and compared within sample types based on litter condition using PCR and denaturing gradient gel electrophoresis (PCR-DGGE). The microbiotas were further analyzed using 16S rRNA gene clone libraries constructed from microbiota DNA extracted from both chick intestinal and litter samples collected at day 7. Results showed significant reciprocal effects between the microbiotas present in the litter and those in the intestines of broilers. Fresh litter had more environmental bacteria, while reused litter contained more bacteria of intestinal origin. Lactobacillus spp. dominated the ileal mucosal microbiota of fresh-litter chicks, while a group of bacteria yet to be classified within Clostridiales dominated in the ileal mucosal microbiota in the reused-litter chicks. The Litter condition (fresh versus reused) seemed to have a more profound impact on the ileal microbiota than on the cecal microbiota. The data suggest that the influence of fresh litter on ileal microbiota decreased as broilers grew, compared with temporal changes observed under reused-litter rearing conditions.

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“…This benefit using ELISA was less clear with cloacal swabs. As litter is bactericidal to Salmonellae (OLESIUK et al, 1971), it is possible that numerous dead bacterial cells were present in the litter. Because the ELISA test detects Salmonella antigen, it is possible that these dead bacterial cells contributed to positive absorption values, while bacterial growth was not possible.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Salmonella‐Tek ELISA to Culture Methods for Detection of Salmonella enteritidis in Litter and Cloacal Swabs of Poultry

Desmidt

Haesebrouck

Ducatelle

1994

Journal of Veterinary Medicine, Series B

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An enzyme-linked immunosorbent assay (ELISA), using the Salmonella-Tek ELISA test system, was compared with bacteriological isolation for detection of Salmonella enteritidis phage type 4 in litter and cloacal swabs of poultry. Before ELISA, samples were enriched in tetrathionate broth for 18-24 h or in tetrathionate broth (18-24 h), followed by M-broth/novobiocine (4-6 h). The bacteriological isolation procedure included incubation in buffered peptone water and inoculation on modified semisolid Rappaport-Vassiliadis. For detection of S. enteritidis in litter samples, ELISA was more sensitive than bacteriological examination. For cloacal swabs, both techniques were equally sensitive. The two enrichment procedures gave no significant differences in ELISA results. It was concluded that the Salmonella-Tek ELISA test might be a promising test for detection of Salmonella infected poultry flocks, providing that further studies are performed to determine the cut-off value for litter and cloacal swabs of commercial flocks.'* Corresponding author U.S.

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Inhibitory Effect of Used Litter on Salmonella typhimurium Transmission in the Chicken

Cited by 33 publications

References 6 publications

Impact of the Australian litter re-use practice on Salmonella in the broiler farming environment

Impact of the Australian litter re-use practice on Salmonella in the broiler farming environment

Interrelations between the Microbiotas in the Litter and in the Intestines of Commercial Broiler Chickens

Comparison of the Salmonella‐Tek ELISA to Culture Methods for Detection of Salmonella enteritidis in Litter and Cloacal Swabs of Poultry

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