Inhibitory effect ofEucommia ulmoides Oliv. on oxidative DNA damage in lymphocytes induced by H2O2

Abstract

This study used the alkaline single cell gel electrophoresis assay (comet assay) to investigate the effect of water extracts of roasted cortex and leaves from Du-zhong on DNA damage in lymphocytes induced by H(2)O(2). The results showed that the DNA damage in human lymphocytes increased with an increase in the concentration of H(2)O(2) (0-200 micro;M), but that the water extracts from Du-zhong (0-2 g l(-1)) only slightly affected DNA damage. The inhibitory effect of leaf extract on DNA damage induced by H(2)O(2) in lymphocytes was more significant (P<0.05) than that of roasted cortex. Leaf extract showed a rather significant inhibitory effect in a concentration-dependent manner. At a concentration of 2 g l(-1), the leaf extract inhibited 37.9% DNA oxidative damage in human lymphocytes. In order to elucidate the mechanism of the leaf extract suppression effect on DNA damage induced by H(2)O(2) in lymphocytes, an experiment was divided with six groups (A-F). Group A was used to evaluate the repair ability of the leaf extract for DNA damage; Group B was employed to determine the scavenging ability on H(2)O(2); and Group C was studied to assess the ability of leaf extract to increase the defense capability. Groups D-F were negative controls and blank. The results showed that group B had the best inhibitory effect. Also, leaf extract had significant ability to scavenge H(2)O(2) in an in vitro HRP-phenol red test. Thus, it appears that H(2)O(2) scavenging potency may be the major mechanism whereby leaf extract inhibits oxidative DNA damage induced by H(2)O(2).

Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9

“…Yen and Hsieh (2003) have shown that EuO leaf extract exhibited a rather significant inhibitory effect on DNA damage induced by H 2 O 2 in lymphocytes in a concentration-dependent manner. Ohmori et al (2005) assessed the antioxidant activity of EuO and found that the inhibitory effects of EuO on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and low-density lipoprotein (LDL) oxidation were noticeable in the format of Duzhong tea in vitro.…”

“…Yen and Hsieh (2003) reported that the inhibitory effect of EuO leaf extract on DNA damage induced by H 2 O 2 in lymphocytes was more significant than that of roasted EuO cortex. Luo et al (2009) found that enzyme-assisted water extract is more effective than the ethanol extracts of EuO leaves for antioxidant extraction.…”

“…It is reported that EuO leaves contain antioxidative and free radical scavenging components. EuO could possibly act as a prophylactic agent to prevent free radical-related diseases (Cho et al, 2003;Yen and Hsieh, 2003) and ·OH, which are catalyzed by superoxide dismutase (SOD), and also oxidation enzymes, resulting in cellular damage and apoptosis (Chen et al, 2006). Therefore, it is clear that EuO plays a role in influencing apoptosis.…”

Antioxidant Protection of Edible Oils

“…In addition to potential applications in food preservation this work has relevance also to human health, in that lipid peroxidation within the body causes membrane dysfunction and increased atherogenicity of low density lipoprotein (LDL) [6,7]. Furthermore, lipid degradation products are reported to be carcinogenic and cytotoxic [8,9]. Therefore, the use of traditional herbs with antioxidant properties has potentially valuable applications in both food and health science [10,11].…”

Mechanism of the Inhibitory Effects ofEucommia ulmoidesOliv. Cortex Extracts (EUCE) in the CCl4-Induced Acute Liver Lipid Accumulation in Rats

“…cortex extracts (EUCE) contain the same components as EU leaves, which have been the focus of medical research [2]. Studies have shown that EU leaf extracts have potent protective effects in various lipid peroxidation models and reduce oxidative damage of biomolecules [3, 8–11]. …”