2002
DOI: 10.1002/ijc.10373
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Inhibitory effects of a luteinizing hormone‐releasing hormone agonist on basal and epidermal growth factor‐induced cell proliferation and metastasis‐associated properties in human epidermoid carcinoma a431 cells

Abstract: The life threatening characteristics of cancers lie in the uncontrolled cell mitogenic activity and the acquiring of metastatic capability. Therefore, the strategies for cancer therapy are aimed at controlling the cancer cell growth and/or inhibiting the cancer cell invasion/metastasis. Early reports contended that protein tyrosine kinase (PTK) regulatory mechanisms are clearly implicated in the growth of neoplastic cells, and inactivating specific PTKs could retard the growth of tumors. 1-3 Among the PTKs inv… Show more

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Cited by 32 publications
(28 citation statements)
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“…Commercial sources of antibodies included mouse anti-green fluorescent protein (GFP) from Abcam; rabbit anti-p34-Arc (ArpC2) from Millipore; mouse anti-Abi1 (clone 1B9) from Medical and Biological Laboratories Co. Ltd. (MBL); mouse anti-EGFR and mouse anti-active-EGFR (Tyr(P)-EGFR), R-phycoerythrin-conjugated mouse anti-EGFR (clone EGFR.1 and isotype control clone [27][28][29][30][31][32][33][34][35], all from BD Biosciences; mouse anti-Tyr(P) (PY99) from Santa Cruz Biotechnology; mouse anti-tubulin from Sigma-Aldrich; and goat anti¬FBP17 from IMGENEX. Secondary antibodies included Alexa Fluor 488-or Alexa Fluor 633-conjugated goat anti-mouse/rabbit IgG, and Alexa633-conjugated goat antimouse/rabbit IgG (Molecular Probes).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Commercial sources of antibodies included mouse anti-green fluorescent protein (GFP) from Abcam; rabbit anti-p34-Arc (ArpC2) from Millipore; mouse anti-Abi1 (clone 1B9) from Medical and Biological Laboratories Co. Ltd. (MBL); mouse anti-EGFR and mouse anti-active-EGFR (Tyr(P)-EGFR), R-phycoerythrin-conjugated mouse anti-EGFR (clone EGFR.1 and isotype control clone [27][28][29][30][31][32][33][34][35], all from BD Biosciences; mouse anti-Tyr(P) (PY99) from Santa Cruz Biotechnology; mouse anti-tubulin from Sigma-Aldrich; and goat anti¬FBP17 from IMGENEX. Secondary antibodies included Alexa Fluor 488-or Alexa Fluor 633-conjugated goat anti-mouse/rabbit IgG, and Alexa633-conjugated goat antimouse/rabbit IgG (Molecular Probes).…”
Section: Methodsmentioning
confidence: 99%
“…Several individual colonies for each cell line were recorded at time 0 and 72 h post-EGF treatment using a phasecontrast microscope equipped with a digital camera. Cell invasion assays were performed essentially as previously described (31). Briefly, A431 vector and Toca-1 knockdown cells (1 ϫ 10 5 cells; in triplicate) were placed in the upper chamber of Transwell inserts (8-m pore size) coated with a layer of Matrigel TM (100 l of 1:5 dilution in DMEM).…”
Section: Methodsmentioning
confidence: 99%
“…Equal amounts of lysate protein were run on 12% SDS-PAGE and electrophoretically transferred to PVDF membrane (Amersham Pharmacia Biotech, USA) then the blocked blots were incubated with specific primary antibody against cytochrome c, Apaf-1, AIF, Endo G, caspase-9 and caspase-3 overnight and further incubated for 1 h with HRP conjugated secondary antibody (Santa Cruz Biotechnology). Bound antibodies were detected by ECL kit as described previously (18)(19)(20)(21).…”
Section: Western Blotting Of Apoptosis Associated Proteins Nci-h295 mentioning
confidence: 99%
“…After blocking, the blots were incubated with specific primary antibody against caspase-3, caspase-8, caspase-9, cytochrome c and TRIAL, Bax, Bcl-X, Bid, AIF, Endo G, Chk2, Chk1, Wee 1, Cdc25c, Cdc2, cyclin A and B1 overnight and further incubated for 1 h with HRP conjugated secondary antibody (Santa Cruz). Bound antibodies were detected by ECL kit (Millipore) as described previously (17)(18)(19)(20).…”
Section: Caspase-8 -9 -3 and A Pan-caspase Inhibitors Inhibit Mmeq-mentioning
confidence: 99%