Inhibitory Effects of an Ethanol Extract of Artemisia capillaris on Inflammatory Mediators from LPS-Stimulated RAW 264.7 Cells

Lim, Hee-Kyoung; Cho, Somi K.; Park, Sang Gyu; Cho, Moonjae

doi:10.3839/jksabc.2010.043

Cited by 5 publications

(5 citation statements)

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“…The ethanol extract of A. capillaris has been shown to have high levels of antioxidant activity [ 29 ] and to contain several other bioactive flavonoids, including scoparone, capillarisin, cirsimaritin, genkwanin, and rhamnocitrin [ 30 ]. It has been reported that the ethanol extract of A. capillaris exerted an anti-inflammatory effect on the mRNA expression level of cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells [ 31 ]. COX-2 is an enzyme that generates prostaglandins, which are induced by proinflammatory cytokines and other activators, such as LPS, resulting in the release of a large amount of PGE 2 at inflammation sites [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…During an inflammatory response, a proinflammatory gene such as inducible nitric oxide synthase (iNOS) is catalyzed through signal transduction pathways leading to NO production [ 37 ]. Lim et al [ 31 ] reported that the ethanol extract of A. capillaris suppressed NO production via the downregulation of iNOS transcription. The mRNA and protein levels of iNOS were suppressed markedly by the ethanol extract of A. capillaris treatment in LPS-stimulated RAW 264.7 cells.…”

Section: Resultsmentioning

confidence: 99%

“…The mRNA and protein levels of iNOS were suppressed markedly by the ethanol extract of A. capillaris treatment in LPS-stimulated RAW 264.7 cells. Therefore, the ethanol extract of A. capillaris could be a good raw material for the development of drugs for the treatment of chronic inflammatory diseases [ 31 ]. These results demonstrate that the extraction conditions may contribute to the anti-inflammatory activity of A. capillaris in LPS-induced RAW 264.7 cells and may effectively yield a crude extract with high anti-inflammatory activity.…”

Section: Resultsmentioning

confidence: 99%

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Extraction Optimization for ObtainingArtemisia capillarisExtract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells

Jang

Jeong

Kim

et al. 2015

BioMed Research International

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Plant extracts have been used as herbal medicines to treat a wide variety of human diseases. We used response surface methodology (RSM) to optimize the Artemisia capillaris Thunb. extraction parameters (extraction temperature, extraction time, and ethanol concentration) for obtaining an extract with high anti-inflammatory activity at the cellular level. The optimum ranges for the extraction parameters were predicted by superimposing 4-dimensional response surface plots of the lipopolysaccharide- (LPS-) induced PGE2 and NO production and by cytotoxicity of A. capillaris Thunb. extracts. The ranges of extraction conditions used for determining the optimal conditions were extraction temperatures of 57–65°C, ethanol concentrations of 45–57%, and extraction times of 5.5–6.8 h. On the basis of the results, a model with a central composite design was considered to be accurate and reliable for predicting the anti-inflammation activity of extracts at the cellular level. These approaches can provide a logical starting point for developing novel anti-inflammatory substances from natural products and will be helpful for the full utilization of A. capillaris Thunb. The crude extract obtained can be used in some A. capillaris Thunb.-related health care products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Extraction Optimization for ObtainingArtemisia capillarisExtract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells

Jang

Jeong

Kim

et al. 2015

BioMed Research International

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“…The DXM-treated group was considered as the positive control. Numerous studies have shown that crude extracts and compounds isolated from AC possess anti-inflammatory effects in different cell types [ 21 , 22 , 34 , 60 ]. In parallel, our data demonstrate that HPAC reduces inflammatory responses in LPS-activated macrophage cells.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

Ali¹,

Lim

Kim

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Growing evidence suggests that dietary nutrients in herbs and plants are beneficial in improving inflammatory disorders. Artemisia capillaris Thunberg (AC) is a traditional herbal medicine widely used in East Asia to treat pain, hepatotoxicity, and inflammatory disorders. Heat processing is a unique pharmaceutical method used in traditional herbal medicine to enhance the pharmacological effects and safety of medicinal plants. This study demonstrates the anti-inflammatory effects of heat-processed AC (HPAC) in lipopolysaccharide- (LPS-) treated mouse macrophage cells. HPAC reduced LPS-induced inflammatory mediators such as IL-6, IL-1β, TNF-α, NO, and PGE2 in RAW 264.7 cells. Interestingly, 15-PGDH appears to play a pivotal role rather than COX-2 and mPGES-1 when HPAC regulated PGE2 levels. Meanwhile, HPAC showed anti-inflammatory effects by blocking IκBα phosphorylation and NF-κB nuclear translocalization. Also, we found that HO-1 upregulation was mediated by the mitogen-activated protein kinase (MAPK) pathways in HPAC-treated RAW 264.7 cells. And, in RAW 264.7 cells challenged with LPS, HPAC restored HO-1 expression, leading to NF-κB inhibition. Through further experiments using specific MAPK inhibitors, we found that, in response to LPS, the phosphorylated IκBα and activated NF-κB were attenuated by p38 MAPK/HO-1 pathway. Therefore, HPAC targeting both the IκBα/NF-κB complex and 15-PGDH may be considered as a potential novel anti-inflammatory agent derived from a natural source.

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“…Cells were irradiated with UV in PBS and then treated with glycitin or DMSO for 48 h. MTT solutions (10 µL of 5 mg/ mL solution; Amresco) was added to each well and incubated at 37 o C for 4 h. Subsequently, the medium was gently removed and replaced with 150 µL of DMSO and incubated for 30 min with shaking to dissolve the precipitate. Absorbances of the samples were measured at 570 nm with a spectrophotometer (Lim et al, 2010). Western blotting.…”

Section: Methodsmentioning

confidence: 99%

The protective effect of glycitin on UV-induced skin photoaging in human primary dermal fibroblast

Seo

Park

Huh

et al. 2014

J Korean Soc Appl Biol Chem

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Exposure of strong and repeated UV on the skin leads to skin aging, characterized with wrinkling, sagging, dyspigmentation, and laxity. Numerous studies revealed that Matrix metalloproteinases are related to skin aging and functions as degrading enzyme of various types of collagen. Here, we attempted to evaluate the effectiveness of glycitin (4'-hydroxy-6-methoxyisoflavone-7-D-glucoside) on skin aging and mechanisms of action in UV-irradiated human dermal fibroblasts. Especially we focused on the expression of Matrix metalloproteinase-1 (MMP-1), which degrades procollagen type-I in dermis, by 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Western blot, and reverse transcription polymerase chain reaction in cell lysates or media. Our results showed that glycitin increased the viability of human dermal fibroblast and alleviated MMP-1 expression caused by UV irradiation. In addition, synthesis of type-I collagen was increased and UV-induced phosphorylation of ERK/JNK/p38 was decreased in dose-dependent manners. Taken together, we demonstrated that treatment with glycitein have a protective effect on skin aging by inhibiting of MMP-1 and increasing of collagen through ERK/JNK/P38 down-regulation, which may be mediated by the inhibition of ERK, JNK, and p38 mitogen-activated protein kinases. We suggest that glycitin is a potential agent for the treatment of skin ageing.Keywords 4'-hydroxy-6-methoxyisoflavone-7-D-glucoside • collagen type-I • human dermal fibroblast • matrix metalloproteinase-1 • skin ageing • UV

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Inhibitory Effects of an Ethanol Extract of Artemisia capillaris on Inflammatory Mediators from LPS-Stimulated RAW 264.7 Cells

Cited by 5 publications

References 22 publications

Extraction Optimization for ObtainingArtemisia capillarisExtract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells

Extraction Optimization for ObtainingArtemisia capillarisExtract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells

Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

The protective effect of glycitin on UV-induced skin photoaging in human primary dermal fibroblast

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