Inhibitory effects of anticancer peptide from Mercenaria on the BGC‐823 cells and several enzymes

Abstract

An anti-cancer peptide was purified from the Mercenaria (Meretrix meretrix Linnaeus) by the method of chromatography on Sephadex G-25 and FPLC, and its molecular weight was determined to be 3147 Da by the way of MALDI-TOF mass spectrum. The effects of this peptide on human gastric gland carcinoma cells (BGC-823) and their cytoskeletal morphology were investigated. The results showed that the peptide could inhibit the proliferation of BGC-823 cells and obviously destroy the skeletal structures of the cells. When the concentration of the peptide reached 4.0 lg/ml, the inhibition percentage of the cell growth was about 60%. The effects of this anticancer peptide on the activities of superoxide dismutase (SOD), alkaline phosphatase (ALP) and tyrosinase were studied. The results showed that the peptide activated ALP and SOD, but inhibit the tyrosinase activity. When the concentration of the peptide reached to 0.5 lg/ml, the relative activities of SOD, ALP and tyrosinase were determined to be 188.5%, 122.0% and 27.5%, respectively.

Antioxidant and cytotoxic potential of a new thienyl derivative fromTagetes erectaroots

“…In addition to its fatal effects on human body such as tissue degeneration, ROS may also join in generation as well as in malign transformation of cancer cells. If newer compounds from natural sources can enhance the level of antioxidation within the body and clear the ROS in cancer cells, they may inhibit the cell's growth and may thereby play a vital role to treat cancer in combination with the anticancer drugs (Lee et al, 2003;Leng et al, 2005). In our research, the ethanol extract of T. erecta was found to scavenge the DPPH free radical indicating that this property may contribute equally or enhance its cytotoxic potential.…”

Mere15, a novel polypeptide fromMeretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs

“…Several peptides and proteins derived from M. meretrix with antitumor activity have been isolated; Leng et al (2005) demonstrated a peptide extracted from M. meretrix significantly inhibited the proliferation of human gastric gland carcinoma cells by affecting activities of SOD, ALP and tyrosinase. In our previous study, two peptides have been isolated form M. meretrix, in which MML and Mere15 exhibited high anticancer activity (Ning et al, 2009;Wang et al, 2012).…”

A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization

“…Several peptides and proteins with antitumor activity have also been extracted from M. meretrix. Leng et al purified a peptide from M. meretrix, which exhibited strongly inhibitory effect on the growth of human stomach adenocarcinoma BGC-823 cells (18). In addition, two glycoproteins, MGP0405 (MW: 9,655 Da) and MGP0501 (MW: 15,878 Da), were reported to have anti-tumor effect in vitro (19,20).…”

“…There is evidence to show that M. meretrix has anti-cancer efficacy, eliminating cysts and detoxification (15)(16)(17)(18). Several peptides and proteins with antitumor activity have also been extracted from M. meretrix.…”