Inhibitory effects of Baccharis dracunculifolia on 1,2-dimethylhidrazine-induced genotoxicity and preneoplastic lesions in rat colon

Abstract

Baccharis dracunculifolia (Asteraceae), the main botanical source of green propolis, also known as 'alecrim-do-campo' and 'vassourinha', is a shrub of the Brazilian 'cerrado' and is native to the South and Southeast of Brazil. The effects of B. dracunculifolia ethyl acetate extract (Bd-EAE) were evaluated on the 1,2-dimethylhydrazine (DMH)-induced DNA damage and aberrant crypt foci (ACF) in the colon of male Wistar rats by the comet and ACF assays, respectively. The animals were treated by gavage with doses of 6, 12, and 24 mg/kg body weight/day. Animals were also administered a single subcutaneous injection of 40 mg/kg DMH and were killed after 4 h for evaluation of DNA damage. Also, two doses of 40 mg/kg of DMH were administered weekly for 2 weeks, and animals were killed 2 weeks after the last injection for evaluation of ACF development in the colon. The results showed a significant reduction in the frequency of DNA damage and ACF in the group treated with the Bd-EAE plus DMH in comparison with those treated with DMH alone, suggesting that Bd-EAE reduced DNA damage and suppressed the formation of ACF and also exerted a protective affect against colon carcinogenesis.

Cellular and molecular effects of Baccharis dracunculifolia D.C. and Plectranthus barbatus Andrews medicinal plant extracts on retinoid metabolism in the human hepatic stellate cell LX-2

“…These results corroborate Munari et al (2009) [39], who studied the antigenotoxic effects of B. dracunculifolia ethanol extract on lung fibroblasts of Chinese hamsters (V 79 cells) exposed to 200 μM of methyl methanesulfonate (MMS) which confirmed the genoprotective capability of the extract. Moreover, our results are in accordance with Roberto et al (2016) [21], who observed the hepatoprotective capability of B. dracunculifolia metabolic products against genotoxic agents [40]. According to Tapia et al (2004) and Resende et al (2007), the protective effects of some Baccharis species can be attributed to the antioxidant activities of their active phenolic components [41, 42].…”

Efficacy of Baccharis dracunculifolia in the treatment of diversion colitis in rats

“…Jaramillo-Garcia et al 18 studied another rosemary species, Baccharis trinervis, to evaluate the toxicity and mutagenic properties of the plant, confirming some of these harmful effects with this plant. Munari et al 19 demonstrated that Baccharis dracunculifolia prevented DNA damage and aberrant crypt formation in the intestinal mucosa of rats exposed to 1.2-dimethylhydrasine, an inducer of genotoxicity and preneoplastic lesions. In the present study no cellular alterations that could suggest mutations or other type of damage were observed, but new researches should be performed to confirm the safety of the extract.…”

Análise da prospecção tecnológica por meio de revisão sistemática seguida de meta análise das patentes do gênero Baccharis

“…• Ação antidiabética e sensibilizadores de insulina (CHOI et al, 2011, IKEDA et al, 2011UEDA et al, 2013) • Oncológica (KIMOTO et al, 2000;KIMOTO et al, 2001a, KIMOTO et al, 2001bAKAO et al, 2003, SHIMIZU et al, 2005SHIMIZU et al, 2006;CHEN et al, 2009aCHEN et al, , 2009bMESSERLI et al, 2009;VOELKEL-JOHNSON, 2011;SZLIS-ZAKA et al, 2011b;FRANCHI et al, 2012;ISHIKAWA et al, 2012;SZLISZKA et al, 2012;DE OLIVEIRA 2013;KIM et al, 2014;MUNARI et al, 2014;FRIÓN-HERRERA et al, 2015, MORTAZAVI et al, 2015, YANG et al, 2017, YOON et al, 2017SOUZA et al, 2018) e…”