Inhibitory effects of bFGF, VEGF and minoxidil on collagen synthesis by cultured hair dermal papilla cells

Lachgar, S.; Charvéron, M.; Bouhaddioui, N; Neveux, Y; Gall, Y.; Bonafé, J L

doi:10.1007/bf02505237

Cited by 28 publications

(19 citation statements)

References 16 publications

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“…HaCaT cells were seeded into 96-well plates at 1.5ϫ10 4 cells/well, cultured for 24 h in serum-free DMEM, and treated with the vehicle (0.12 mM HCl/DMSO diluted 1 : 1000 in serum-free DMEM) as control, minoxidil (0.01 mM), retinol (1.7 pM-1.7 nM), or minoxidil (0.01-1.0 mM) plus retinol (1.7 pM-1.7 nM) for 5 d. Cell growths and viabilities were measured by adding 0.4% trypan blue in 0.9% saline to a 50% dilution, and cells were counted using a hemocytometer. Western Blot Confluent DPCs and HaCaT were incubated for 24 h treated with vehicle, minoxidil (0.01 mM), retinol (1.7 pM), or 0.01 mM minoxidil plus 1.7 pM retinol for 1 h to evaluate Erk and Akt expression and for 24 h to evaluate Bcl-2/Bax ratios and b-actin expression.…”

Section: )mentioning

confidence: 99%

The Additive Effects of Minoxidil and Retinol on Human Hair Growth in Vitro

Yoo

Chang

Pyo

et al. 2007

Biological & Pharmaceutical Bulletin

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Minoxidil, a pyrimidine derivative (2,4-diamino-6-piperidino-pyrimidine-3-oxide) is an adenosine triphosphate (ATP) sensitive potassium chennel (K ATP chennel) opener, and is the most commonly used drug for the treatment of androgenetic alopecia.1) Topical minoxidil shortens the telogen phase by induction of the entry of resting hair follicles into the anagen phase, prolongs anagen growth phase and increases the volume of hair follicles. Although the mechanism through which minoxidil promotes hair growth is still speculative, a number of in vitro studies using minoxidil have been described in various skin and hair follicle cell types including the stimulation of cell proliferation, and the induction of vascular endothelial growth factor 2,3) and prostaglandin synthesis.4) In addition, minoxidil has been shown to increase blood circulation around hair follicles 5,6) and to markedly elevate 17b-hydroxysteroid dehydrogenase activity, which accelerated the conversion of testosterone to weaker androgens. 7)Retinol significantly improves the scalp skin condition and promotes hair growth, in terms of the keratinization of skin and hair. 8) Retinol is ingested in precursor forms; animal sources contain retinyl esters whereas plants contain carotenoids. Tissue cells convert these organic precursor forms to retinol, and this to either retinal or retinoic acid. These two species exert profound effects on the growth, maturation, and differentiation of many cell types, both in vivo and in vitro.8) Retinoic acid, the active metabolite of vitamin A, has been reported to play an important role in the growth, differentiation and maintenance of hair follicles. 9) Moreover, cellular retinoic acid-binding proteins (CRBPs) are known to be important for the mediations of some of the effects of retinoids. These effects are mediated by two classes of nuclear retinoic acid receptors, termed retinoic acid receptor (RAR) and retinoid x receptor (RXR), and each of these classes is composed of subtypes designated alpha, beta, and gamma.Recently, we showed that minoxidil prolongs the anagen stage and that the enhanced hair growth may result from two mechanisms: (i) the activations of Erk and Akt signaling which enhance the survival of cultured DPCs, and (ii) an increase in the Bcl-2/Bax ratio which protects cells against cell death.10) The aim of the present study was to evaluate possible additive interaction between minoxidil and retinol on human hair growth and to investigate the mechanism of action of minoxidil plus retinol using cultured human DPCs and keratinocytes. MATERIALS AND METHODS MaterialsMinoxidil and retinol were purchased from Sigma (St Louis, MO, U.S.A.). Minoxidil was dissolved in 0.12 mM HCl and a 1.0 mM minoxidil stock solution was stored at Ϫ20°C. Retinol was dissolved in absolute ethanol and shielded from the light using aluminum foil until required. All inhibitors [U0126, an ERK kinase (MEK1 and 2)-inhibitor; LY 294002 (LY) and Wortmannin, selective PI3K inhibitor] were purchased from Calbiochem (San Diego, CA, U.S.A.)...

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Section: )mentioning

confidence: 99%

The Additive Effects of Minoxidil and Retinol on Human Hair Growth in Vitro

Yoo

Chang

Pyo

et al. 2007

Biological & Pharmaceutical Bulletin

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“…In addition, VEGF is active on DPC metabolism. It affects collagen 11 and eicosanoid 12 production and thus may be implicated in hair growth through these physiological and metabolic pathways.…”

mentioning

confidence: 99%

Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells

Lachgar

Charvéron

Gall

et al. 1998

British Journal of Dermatology

238

132

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The hair follicle dermal papilla which controls hair growth, is characterized in the anagen phase by a highly developed vascular network. We have demonstrated in a previous study that the expression of an angiogenic growth factor called vascular endothelial growth factor (VEGF) mRNA varied during the hair cycle. VEGF mRNA is strongly expressed in dermal papilla cells (DPC) in the anagen phase, but during the catagen and telogen phases. VEGF mRNA is less strongly expressed. This involvement of VEGF during the hair cycle allowed us to determine whether VEGF mRNA expression by DPC was regulated by minoxidil. In addition, the effect of minoxidil on VEGF protein synthesis in both cell extracts and DPC-conditioned medium, was investigated immunoenzymatically. Both VEGF mRNA and protein were significantly elevated in treated DPC compared with controls. DPC incubated with increasing minoxidil concentrations (0.2, 2, 6, 12 and 24 mumol/L) induced a dose-dependent expression of VEGF mRNA. Quantification of transcripts showed that DPC stimulated with 24 mumol/L minoxidil express six times more VEGF mRNA than controls. Similarly, VEGF protein production increases in cell extracts and conditioned media following minoxidil stimulation. These studies strongly support the likely involvement of minoxidil in the development of dermal papilla vascularization via a stimulation of VEGF expression, and support the hypothesis that minoxidil has a physiological role in maintaining a good vascularization of hair follicles in androgenetic alopecia.

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“…However, lengthier body hairs in individuals using minoxidil would indicate that anagen duration may also be prolonged . There is confusion as to the exact mechanism by which minoxidil works; opening potassium ion channels promoting vasodilation, upregulation of VEGF and upregulation of prostaglandin E2 have all been proposed . Regardless, if minoxidil simply accelerates hair cycle progression and does not impact efflux or influx, it would only slow the progression of miniaturisation and/or reduce the appearance of baldness by increasing the number of hair follicles in anagen at any given point in time.…”

Section: Are We Manipulating Miniaturisation or Simply Accelerating Tmentioning

confidence: 99%

“…opening potassium ion channels promoting vasodilation, upregulation of VEGF and upregulation of prostaglandin E2 have all been proposed. [52][53][54][55] Regardless, if minoxidil simply accelerates hair cycle progression and does not impact efflux or influx, it would only slow the progression of miniaturisation and/or reduce the appearance of baldness by increasing the number of hair follicles in anagen at any given point in time.…”

Section: Are We Manipul Ating Miniaturisation or S Imply Acceler Atmentioning

confidence: 99%

A bald statement — Current approaches to manipulate miniaturisation focus only on promoting hair growth

Pantelireis

2018

Experimental Dermatology

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Hair plays a large part in communication and society with its role changing through time and across cultures. Most people do not leave the house before combing their hair or shaving their beard and for many hair loss or irregular hair growth can have a significant impact on their psychological health. Somewhat unsurprisingly, according to GMR Data, today's global hair care industry is worth an estimated $87 Billion, with hair loss estimated at $2.8 Billion. Considering that no current hair loss-related products can completely reverse hair loss, it is reasonable to believe this market could expand significantly with the discovery of a comprehensive therapy. As such, a great deal of research focuses on overcoming hair loss, and in particular, a common form of hair loss known as androgenetic alopecia (AGA) or male pattern baldness. In AGA, hair follicles miniaturise in a large step change from a terminal to a vellus state. Within this viewpoint article, we discuss how influx and efflux of cells into and out from the dermal papilla (DP) can modulate DP size during the hair cycle. As DP size is positively correlated with the size of the hair fibre produced by a follicle, we argue here that therapies for treating AGA should be developed which can alter DP size, rather than just promote hair growth. We also discuss current therapeutics for AGA and emphasise the importance of using the right model systems to analyse miniaturisation.

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Inhibitory effects of bFGF, VEGF and minoxidil on collagen synthesis by cultured hair dermal papilla cells

Cited by 28 publications

References 16 publications

The Additive Effects of Minoxidil and Retinol on Human Hair Growth in Vitro

The Additive Effects of Minoxidil and Retinol on Human Hair Growth in Vitro

Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells

A bald statement — Current approaches to manipulate miniaturisation focus only on promoting hair growth

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