Inhibitory Effects of Bovine Lactoferrin on the Adherence of Enterotoxigenic<i>Escherichia coli</i>to Host Cells

Kawasaki, Yuki; Tazume, Seiki; Shimizu, Keiko; Matsuzawa, Hideyuki; Dosako, Shun’ichi; Isoda, Hiroko; Tsukiji, Mami; Fujimura, Ryuko; Muranaka, Yoko; Isihida, Hiroshi

doi:10.1271/bbb.64.348

Cited by 55 publications

(37 citation statements)

References 29 publications

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“…[53] -apo-hLf caused cell lysis -The mechanism involved was caused by the binding of proteins to amoeba membrane E. histolytica HM 1:IMSS bLf, apo-hLf and bLfcin (6) (fragment [4][5][6][7][8][9][10][11][12][13][14] [ ]* 1.0, 2.5 -bLf and hLf were amoebicidal [54] -Effect was concentration-dependent and modulated by environmental conditions -bLf was more effective than bLfcin -All components synergized with metronidazole vs amoebae E. histolytica HM 1:IMSS bLfcin, bLfampin and bLf chimera (7) [ ]* 0.025, 0.050, 0.075, 0.10 -bLfcin and bLfampin had a moderate amoebicidal effect [55] -bLfcin chimera showed the highest microbicidal activity -The microbicidal effect of Lf peptides was iron-independent G. duodenalis bLf, bLfcin, hLf, Lfcin (2) [ ]* bLfcin and hLfcin: 0-0.024; bLf and hLf: 0-2.5 -LD50: bLfcin: 8 (µg/ml); bLf: 1.2 (mg/ml); hLfcin: 16 (µg/ml); hLf: 1.5 (mg/ml)…”

Section: In Vitro Assaysmentioning

confidence: 99%

“…In addition, Lf is bactericidal, by binding to lipopolysaccharide (LPS), porins, and other outer membrane (OM) proteins in Gram-negative bacteria [6]; likewise, Lf can bind to teichoic acid in Gram-positive bacteria. All these actions cause disruption of the bacterial membrane, leading to cell lysis [7,8] bLf affects biofilm production [9][10][11], diminishes the release of toxins [12], and interferes with the adhesion of bacteria to host cells [9,[13][14][15][16]. Moreover, Lf synergizes with antibiotics, potentiating their antimicrobial effect [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Luna-Castro¹,

Samaniego-Barrón²,

Serrano-Rubio³

et al. 2017

J Adv Dairy Res

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Lactoferrin (Lf) is an iron-chelating glycoprotein present in milk and mucosal secretions, a component of the mammalian innate immune system. Lf is microbiostatic and microbicidal. Lf can reduce the bacterial expression of virulence factors, such as those involved in biofilm production and protease secretion. The high identity among mammalian Lf sequences facilitates its use in human and veterinary medicine. Lf of bovine origin is the principal Lf used due to its commercial availability through purification from milk whey; recombinant Lfs (bovine, human, and porcine) have been used as well. Lf is a stable protein that retains its physicochemical characteristics under gastric pH conditions, and in most cases it is bioactive even after digestion; thus, the incorporation of Lf into diets facilitates its administration to animals. The aim of this review is to examine original research in which the effects of bovine and porcine Lf on pathogens of domestic animals have been demonstrated through in vitro and in vivo assays, with the purpose of ascertaining the benefits that Lf provides in the treatment of infectious diseases.

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Section: In Vitro Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Luna-Castro¹,

Samaniego-Barrón²,

Serrano-Rubio³

et al. 2017

J Adv Dairy Res

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“…Binding to E. coli colonization factors [36] causes inhibition of hemagglutination [37] and inhibition of adherence of ETEC to epithelial cells in vitro and to intestinal mucosa of germfree mice in vivo [38].…”

Section: Enterotoxigenic Escherichia Coli (Etec)mentioning

confidence: 99%

Effect of lactoferrin on enteric pathogens

Ochoa

Cleary²

2009

Biochimie

119

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Much has been learned in recent years about the mechanisms by which breastfeeding improves child health and survival. However, there has been little progress in using these insights to improve pediatric care. Factors that are important for protecting the breast fed infant might be expected to decrease the adverse effects of weaning on diarrhea, growth, and development. Lactoferrin, an ironbinding protein with multiple physiological functions (anti-microbial, anti-inflammatory, and immunomodulatory), is one of the most important proteins present in mammalian milk. Protection against gastroenteritis is the most likely biologically relevant activity of lactoferrin. Multiple in vitro and animal studies have shown a protective effect of lactoferrin on infections with enteric microorganisms, including rotavirus, Giardia, Shigella, Salmonella and the diarrheagenic Escherichia coli. Lactoferrin has two major effects on enteric pathogens: it inhibits growth and it impairs function of surface expressed virulence factors thereby decreasing their ability to adhere or to invade mammalian cells. Thus, lactoferrin may protect infants from gastrointestinal infection by preventing the attachment by enteropathogens in the gut. Recently several clinical trials in children have started to address this issue. Whether lactoferrin can prevent a significant portion of diarrheal disease remains to be determined.

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“…Sansonetti (39) recently reviewed host-pathogen interactions and highlighted the diversity in the mechanisms of invasion of pathogenic bacteria. In that review, it was described that "[w]hen invasive microorganisms bind to the intestinal epithe- (40) showed antiinfectious effects of lactoferrin on the adherent bacterial count. On the other hand, the increased weight of the small intestine of AR compared with that of MR pups was histologically investigated, and the villi and smooth muscle layer was found to be more developed in the AR pups (19).…”

mentioning

confidence: 99%

Intestinal Adherent Bacteria and Bacterial Translocation in Breast-Fed and Formula-Fed Rats in Relation to Susceptibility to Infection

et al. 2003

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The barrier function of the intestinal mucosa is immature in the newborn mammal, and is strengthened by breast milk. We investigated this effect of breast milk by comparing the susceptibility to infection assessed in terms of adherent bacterial colonization of the intestinal tissue (AdC) and bacterial translocation (BT) between breast-fed and formula-fed newborn rats. Threeday-old rat pups were assigned to one of three groups: motherreared (MR), pseudo-cannulated (sham), and artificially reared (AR). AR rats were infused with formula through an intragastric cannula, under the control of a computer-regulated pumping machine. MR and sham rat pups were reared with their respective dams and received breast milk until weaning in a specially designed cage. In 10-d-old rats, there was no significant difference in the fecal or cecal flora between the AR and MR groups, whereas the AdC and the BT to the liver were greater in the AR than MR group. Enterobacteriaceae, Streptococcus and/or Enterococcus, and Staphylococcus were dominantly detected as microorganisms in AdC flora and BT. The AdC flora did not directly reflect the bacterial colonization flora. These findings suggest that AR rat pups mature normally, although there is a greater colonization of Enterobacteriaceae and BT in AR than MR pups. Consequently, the intestinal barrier function of the pups reared by artificial feeding may become susceptible to BT, and AdC may be more indicative than bacterial colonization of the susceptibility to BT. Recent advances in the quality of infant formulas have increased their similarity to human breast milk. Moreover, there are no major differences in the physical appearance of breast-fed and formula-fed infants. However, breast-fed infants have a lower morbidity and mortality than formula-fed infants. Studies have demonstrated significant infant protection against diarrhea, respiratory tract infections, otitis media, bacteremia, bacterial meningitis, botulism, urinary tract infections, necrotizing enterocolitis, and other diseases while the infant is receiving breast milk (1-5). There is also good evidence of continued protection against these medical conditions for years after breast-feeding has terminated (1, 6 -11).We previously reported in a rat study that BT to the liver was greater in formula-fed AR rat pups than in breast-fed rat pups (12). It was suggested that the outcome is the result of a difference in the intestinal microflora and maturation of the intestinal mucosa.There are reports on the development of the intestinal microflora in newborn animals, and studies have compared the intestinal microflora of breast-fed and formula-fed newborns. However, few reports have examined the role of the intestinal microflora and adherent bacteria in relation to infectious risks to the newborn during the suckling and weaning periods. In aerobic fecal microflora in human milk-fed and formula-fed infants in the intensive care unit, Staphylococcus was most dominantly detected at greater than 60% (13). There have been many studies abo...

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Inhibitory Effects of Bovine Lactoferrin on the Adherence of EnterotoxigenicEscherichia colito Host Cells

Cited by 55 publications

References 29 publications

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Lactoferrin: A Powerful Antimicrobial Protein Present in Milk

Effect of lactoferrin on enteric pathogens

Intestinal Adherent Bacteria and Bacterial Translocation in Breast-Fed and Formula-Fed Rats in Relation to Susceptibility to Infection

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