Inhibitory Effects of Carnosic Acid on Melanin Synthesis

Abstract

Purpose: This research was carried out to ascertain the whitening effects of carnosic acid on a B16F1 mouse melanoma cell line.Methods: It involved quantitative assessments based on cell viability and assays of gene expression, tyrosinase enzyme activity, and melanin contents. The effects of carnosic acid on tyrosinase (TYR)/microphthalmia-associated transcription factor (MITF)/ tyrosinase-related protein 1 (TYRP1)/TYRP2 messenger ribonucleic acid expression levels and melanin content were determined.Results: Real-time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR) showed that carnosic acid decreased the expression levels of TYR/MITF/TYRP1/TYRP2 and melanin content compared to alpha-melanocite-stimulating hormone-treated B16F1 cells. The tyrosinase activity assay also revealed that carnosic acid decreased melanin production in B16F1 cells.Conclusion: These data indicate that carnosic acid increases the whitening effects on the B16F1 cells. Therefore, carnosic acid is a potential ingredient that can be used for skin whitening. Additionally, further research on the mechanisms of the action of carnosic acid should be conducted for the development of functional foods, cosmetics, and medicines.

Effect of Detox Lifestyle on Skin Care Satisfaction and the Client Quality of Life at a Skin Care Salon

“…삶의 질이란 건강과 관련된 삶의 질을 측정하기 위하여 Shor-Form 36 Item Health Survey Version 2 (SF-36v2)를 이용하였다 (Shim & Jang, 2007). 이 척도는 건강관련 삶의 질(health-related quality of life) 평가에 가장 널리 사용되는 도구로 일반적인 건강, 신 체적 기능, 신체적 역할제한, 통증, 활력, 사회적 기능, 정신건강, 감 정적 역할 제한 등의 다양한 영역을 신체적 건강요약지수와 정신적 건강요약지수의 두 가지 영역으로 나누어 점수를 산출하며 점수가 높 을수록 삶의 질이 높아진 것으로 판정한다 (Lee, 2009).…”