Inhibitory Effects of Chemical Constituents from Actinidia kolomikta on LPS-Induced Inflammatory Responses

Abstract

Sixteen known compounds, including three lignan glycosides (1-3), seven phenolic glycosides (4-10), three glycosides (11-13), and three flavonoids (14-16), were isolated from the rhizomes of Actinidia kolomikta (Rupr. & Maxim.) Maxim., Actinidiaceae. Their structures were elucidated through extensive spectroscopic analyses and comparison with those reported in the literature. This study is the first confirmation of the presence of the twelve compounds (1-4, 6-13) isolated from the Actinidiaceae family. The isolated compounds were evaluated for their inhibitory effects on NO, TNF-α, and IL-6 production in LPS-induced RAW264.7 cells. Among them, salidroside (9), D-threo-guaiacylglycerol 7-O-β-D-glucopyranoside (10), and epi-afzelechin (15) exhibited the potent anti-inflammatory effect against NO, TNF-α, and IL-6. In addition, compound 10 showed significant inhibitory effects on COX-2 expression at 50 μM, and compound 15 showed significant inhibitory effects on iNOS expression at 50 μM. These results suggest that the anti-inflammatory activity of A. kolomikta might in part occur via the inhibition of the expression of the pro-inflammatory cytokines.